forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.

Crystallization of SHARPIN using an automated two-dimensional grid screen for optimization

B. Stieglitz, K. Rittinger and L. F. Haire

Synopsis: The expression, purification and crystallization of an N-terminal fragment of SHARPIN are reported. Diffraction-quality crystals were obtained using a 2-D grid screen seeding technique.

Combining in situ proteolysis and mass spectrometry to crystallize Escherichia coli PgaB

D. J. Little, J. C. Whitney, H. Robinson, P. Yip, M. Nitz and P. L. Howell

Synopsis: Construct engineering and crystallization of E. coli PgaB using in situ proteolysis and mass spectrometry.

Expression, purification, crystallization, and preliminary X-ray analysis of the d-alanyl carrier protein DltC from Staphylococcus epidermidis

C.-H. Huang, C.-H. Kao, C.-S. Yang, C.-H. Chang, S.-C. Chen, S.-M. Kuan, Y.-C. Su, Y.-H. Huang, M.-C. Chang and Y. Chen

Synopsis: The Staphylococcus epidermidis carrier protein DltC has been crystallized in order to elucidate the functional role of DltC in the alanylation of lipoteichoic acids in bacteria.

Expression, purification, crystallization, and preliminary X-ray analysis of the human NORE1 SARAH domain

H. J. Kim, E. Hwang, Y.-H. Han, S. Choi, W. C. Lee, H.-Y. Kim, Y. H. Jeon, C. Cheong and H.-K. Cheong

Synopsis: Crystallization of the human NORE1 SARAH domain

Purification, crystallization, and preliminary X-ray diffraction analysis of effector protein PevD1 from Verticillium dahliae

L. Han, Z. Liu, X. Liu and D. Qiu

Crystallization and preliminary X-ray crystallographic analysis of an ice-binding protein (FfIBP) from Flavobacterium frigoris PS1

H. Do, J. H. Lee, S. G. Lee and H. J. Kim

Synopsis: An ice-binding protein, FfIBP, from Flavobacterium frigoris PS1 was over-expressed, purified and characterized. In addition, the crystallization and preliminary X-ray crystallographic analysis of this ice-binding protein were performed.

Structure of the RBD-PRDI fragment of the antiterminator protein GlcT

S. Himmel, C. Grosse, S. Wolff, S. Schweigk and S. Becker

Crystallization and preliminary X-ray diffraction analysis of a high molecular weight ketoacyl reductase FabG4, complexed with NADH

D. Dutta, S. Bhattacharyya and A. K. Das

Synopsis: Cloning, overexpression, purification, crystallization and preliminary X - ray diffraction analysis of a High Molecular weight Ketoacyl reductase FabG4:NADH complex in triclinic space group.

Expression, purification, crystallization and preliminary crystallographic analysis of the phosphoglycerate kinase from Acinetobacter baumannii

K. Baretta, C. Garen, J. Yin and M. N. G. James

Purification, crystallization and preliminary crystallographic analysis of the adhesion domain of Epf from Streptococcus pyogenes

C. Linke, N. Siemens, M. J. Middleditch, B. Kreikemeyer and E. N. Baker

Synopsis: The putative adhesion domain of the multidomain protein Epf from Streptococcus pyogenes has been crystallized in space groups P2₁ and P2₁2₁2₁. The crystals diffracted to 2.0 and 1.6 Å, respectively, at the Australian Synchrotron.

Crystallization and X-ray diffraction studies of white Pacific shrimp Litopenaeus vannamei arginine kinase

A. A. Lopez-Zavala, R. R. Sotelo-Mundo, K. D. Garcia-Orozco, F. Isac-Martinez, L. G. Brieba and E. Rudiño-Piñera

Structure of the signal transduction protein TRAP (Target of RNAIII-activating protein)

K. Henrick and M. Hirshberg

Synopsis: PDB Reference: 4ae5

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1

K. Kato, H. Nishimasu, E. Mihara, R. Ishitani, J. Takagi, J. Aoki and O. Nureki

Synopsis: The extracellular region of mouse Enpp1 was expressed, purified and crystallized. An X-ray diffraction dataset was collected at 3.0 Å resolution, by employing a helical data collection strategy involving a micro-focus synchrotron beam.

Cloning, purification, crystallization and preliminary X-ray diffraction crystallographic study of acyl-protein thioesterase 1 from Saccharomyces cerevisiae

Y. Yuan, X. Wang, X. Li, M. Teng, L. Niu and Y. Gao

Synopsis: This paper reports the cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of acyl-protein thioesterase 1 from Saccharomyces cerevisiae.

Structure of Leishmania major cysteine synthase

P. K. Fyfe, G. D. Westrop, T. Ramos, S. Müller, G. H. Coombs and W. N. Hunter

Synopsis: A crystallographic and biochemical study of Leishmania major cysteine synthase, a pyridoxyl phosphate-dependent enzyme, is reported. The structure, determined to 1.8 Å resolution, reveals that the co-factor has been lost and that a fragment of -poly D-glutamic acid, a crystallization ingredient, is bound in the active site. The enzyme is inhibited by peptides. PDB reference: 4AIR

Crystallization and preliminary X-ray crystallographic analysis of Pz peptidase B from Geobacillus collagenovarans MO-1

H. Nakano, A. Hosokawa, R. Tagawa, K. Inaka, K. Ohta, T. Nakatsu, H. Kato and K. Watanabe

Purification, crystallization and preliminary X-ray diffraction analysis of a hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase (HCT) from Coffea canephora involved in chlorogenic acid biosynthesis

L. A. Lallemand, J. G. McCarthy, S. McSweeney and A. A. McCarthy

Synopsis: A hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl-transferase (HCT) involved in chlorogenic acid biosynthesis in Coffea canephora was crystallized using the vapour-diffusion method. A diffraction data set has been collected to 3.0 Å resolution on the micro focus beamline (ID23-2) at ESRF and a structure solution was obtained using molecular replacement.

Purification, crystallization and preliminary X-ray analysis of non-structural protein 2 (nsp2) from avian infectious bronchitis virus

K. Yu, Z. Ming, Y. Li, C. Chen, Z. Bao, Z. Ren, B. Liu, W. Tao, Z. Rao and Z. Lou

Synopsis: The non-structural protein 2 from avian infectious bronchitis virus has been overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to a 2.8 Å resolution.

Crystallization of an atypical short-chain dehydrogenase from Vibrio vulnificus missing the conserved catalytic tetrad

G. Buysschaert, K. Verstraete, S. N. Savvides and B. Vergauwen

Crystallization and preliminary X-ray crystallographic analysis of Thermotoga maritima CheA P3-P4-P5 domains and CheW complex

S. Park, K. Y. Kim, S. Kim and B. R. Crane

Synopsis: T. maritima CheA P3-P4-P5 domains were crystallized in complex with CheW. Low-resolution diffraction data to 8 Å were collected using synchrotron X-ray radiation.

Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of cyclophilin A-like protein from Piriformospora indica

H. Bhatt, D. K. Trivedi, R. K. Pal, A. K. Johri, N. Tuteja and N. S. Bhavesh

Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis

J. Hou, K. Wojciechowska, H. Zheng, M. Chruszcz, D. R. Cooper, M. Cymborowski, T. Skarina, E. Gordon, H. Luo, A. Savchenko and W. Minor

Synopsis: The crystal structure of a short-chain dehydrogenase/reductase from B. anthracis strain `Ames Ancestor' is reported.

Crystallization and preliminary X-ray analysis of a novel halotolerant feruloyl esterase identified from a soil metagenomic library

S. Chen, K. Wang, Y. Liu and X. Hu

Purification, crystallization and preliminary X-ray diffraction data of UDP-galactopyranose mutase from Aspergillus fumigatus

G. A. Penman, D. E. A. Lockhart, A. Ferenbach and D. M. F. van Aalten

Synopsis: The cloning, overexpression, purification, crystallization and preliminary X-ray diffraction data are described for UDP-galactopyranose mutase, an enzyme involved in cell-wall synthesis in A. fumigatus.

Structure of a monomeric variant of rhodopsin kinase at 2.5 Å resolution

J. J. G. Tesmer, M. R. Nance, P. Singh and H. Lee

Synopsis: The structure of the L166K variant of G protein-coupled receptor kinase 1 has been determined at 2.5 Å resolution in order to determine how a dimer interface observed in prior crystal structures influences the conformation of the enzyme and how the C-terminus is folded while in a monomeric state.

Purification, crystallization and preliminary crystallographic analysis of histone lysine demethylase NO66 from Homo sapiens

X. Zhou, Y. Tao, M. Wu, D. Zhang and J. Zang

Synopsis: The JmjC domain-containing histone demethylase NO66 from H. sapiens was overproduced in E. coli, purified and crystallized. Diffraction data were collected to 2.29 Å resolution.

Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis

A. Lyskowski, M. Tengg, G. Steinkellner, H. Schwab, M. Gruber-Khadjawi and K. Gruber

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the apo form of InsP₅ 2-K from Arabidopsis thaliana

J. I. Baños-Sanz, J. Sanz-Aparicio, C. A. Brearley and B. González

Synopsis: A mutated version of InsP₅ 2-K allows the production of crystals of the apo form and structure determination using X-ray crystallography.

Purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal fragment of the MvfR protein from Pseudomonas aeruginosa

K. Kefala, D. Kotsifaki, M. Providaki, E. G. Kapetaniou, L. Rahme and M. Kokkinidis

Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii

W. Oonanant, J. Sucharitakul, P. Chaiyen and J. Yuvaniyama

Synopsis: The reductase component of p-hydroxyphenylacetate 3-hydroxylase from A. baumannii was overexpressed, purified and crystallized. X-ray diffraction data were collected and processed to 2.3 Å resolution.

Crystallization and preliminary crystallographic studies of CCM3 in complex with the C-terminal domain of MST4

X. Xu, X. Wang, J. Ding and D.-C. Wang

Synopsis: The complex of CCM3 and the C-terminal domain of MST4 has been successfully constructed, purified and crystallized. The crystal diffracted to a resolution of 2.4 Å.

Overexpression, purification, crystallization and preliminary X-ray analysis of putative molybdenum cofactor biosynthesis protein C (MoaC2) from Mycobacterium tuberculosis H37Rv

S. Srivastava, V. K. Srivastava, A. Arora and J. V. Pratap

Cloning, purification, crystallization and preliminary X-ray studies of human ₁-microglobulin

Y. Zhang, Z. Gao, Z. Zhang, M. Luo, A. Huang, Y. Dong and D. Wang

Crystallization and preliminary X-ray diffraction studies of the GhKCH2 motor domain: alteration of pH significantly improved the quality of the crystals

X. Qin, Z. Chen, T. Xu, P. Li and G. Liu

Synopsis: The GhKCH2 motor domain was crystallized and the pH of the crystallization buffer was shown to have a significant effect on the crystal morphology and diffraction quality.

A model for 3-methyladenine recognition by 3-methyladenine DNA glycosylase I (TAG) from Staphylococcus aureus

X. Zhu, X. Yan, L. G. Carter, H. Liu, S. Graham, P. J. Coote and J. Naismith

Synopsis: The structure of 3-methyladenine DNA glycosylase I in complex with 3-methyladenine is reported.

Crystallization and preliminary X-ray analysis of the C-terminal domain of CCM2, part of a novel adaptor protein involved in cerebral cavernous malformations

X. Wang, J. Ding and D. Wang

Synopsis: The purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of CCM2, which is part of a signalling complex, are reported.

Crystallization and preliminary X-ray crystallographic analysis of bacterial tRNA^Sec in complex with seryl-tRNA synthetase

Y. Itoh, S. Sekine and S. Yokoyama

Expression, purification, crystallization and preliminary X-ray analysis of Plasmodium falciparum GTP:AMP phosphotransferase

A. W. L. Law, J. Lescar, Q. Hao and M. Kotaka

Synopsis: P. falciparum GTP:AMP phosphotransferase was expressed in E. coli, purified and crystallized. An X-ray diffraction data set was collected to a resolution of 2.90 Å.

Purification, crystallization and preliminary X-ray analysis of human histidine decarboxylase

H. Komori, Y. Nitta, H. Ueno and Y. Higuchi

Synopsis: Human histidine decarboxylase was crystallized by the sitting-drop vapour-diffusion method. Diffraction data were collected to 1.8 Å resolution.

Structure of human ADAM-8 catalytic domain complexed with batimastat

T. Hall, H.-S. Shieh, J. E. Day, N. Caspers, J. E. Chrencik, J. M. Williams, L. E. Pegg, A. M. Pauley, A. F. Moon, J. M. Krahn, D. H. Fischer, J. R. Kiefer, A. G. Tomasselli and M. D. Zack

Crystallization and preliminary X-ray crystallographic analysis of human peptidylarginine deiminase type III

M. Unno, K. Kizawa, M. Ishihara and H. Takahara

Synopsis: Human peptidylarginine deiminase type III was crystallized using polyethylene glycol monomethylether or polyethylene glycol as a precipitant. The crystals belonged to space group R3, with unit-cell parameters a = b = 114.97, c = 332.49 Å (hexagonal axes), and contained two molecules in an asymmetric unit.

The ParE2-PaaA2 toxin-antitoxin complex from Escherichia coli O157 forms a heterodocecamer in solution and in the crystal

Y. G. J. Sterckx, A. Garcia-Pino, S. Haesaerts, T. Jové, L. Geerts, V. Sakellaris, L. Van Melderen and R. Loris

Synopsis: Crystals of the complex of E. coli O157 ParE2 and PaaA2 belonged to space group P3₁21 or P3₂21 and diffracted to 3.8 Å resolution.

Crystallization and preliminary crystallographic studies of both components of the staphylococcal LukE-LukD leukotoxin

R. Galy, F. Bergeret, D. Keller, L. Mourey, G. Prévost and L. Maveyraud

Synopsis: The expression, purification and crystallization of the soluble forms of LukE and LukD, which together constitute the LukE-LukD staphylococcal leukotoxin, are described.

Crystallization and preliminary X-ray diffraction of malate dehydrogenase from Plasmodium falciparum

C. Wrenger, I. B. Müller, S. Butzloff, R. Jordanova, S. Lunev and M. R. Groves

Synopsis: The collection of X-ray diffraction data from malate dehydrogenase from P. falciparum to a resolution of 2.2 Å is reported.

Crystallization and preliminary X-ray analysis of the haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58

T. Mase, H. Yabuki, M. Okai, J. Ohtsuka, F. L. Imai, Y. Nagata and M. Tanokura

Synopsis: The haloalkane dehalogenase DatA from A. tumefaciens C58 was expressed, purified and crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected to 1.70 Å resolution.

Purification, crystallization and preliminary X-ray crystallographic analysis of the ATPase domain of human TAP in nucleotide-free and ADP-, vanadate- and azide-complexed forms

S. R. Meena, S. P. Gangwar and A. K. Saxena

Structure of the GDP-bound G domain of the RGK protein Rem2

P. Reymond, A. Coquard, M. Chenon, M. Zeghouf, A. El Marjou, A. Thompson and J. Ménétrey

Crystallization and preliminary crystallographic analysis of D-aspartate oxidase from porcine kidney

M. Senda, A. Yamamoto, H. Tanaka, T. Ishida, K. Horiike and T. Senda

Synopsis: D-Aspartate oxidase from porcine kidney was crystallized by the sitting-drop vapour-diffusion method. The crystal diffracted to 1.80 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of the Bag2 amino-terminal domain from Mus musculus

R. Page, Z. Xu, J. Amick, J. C. Nix and S. Misra

Synopsis: The amino-terminal domain of the Hsp70 co-chaperone Bag2 from M. musculus has been crystallized in native and selenomethionyl forms diffracting to 2.27 and 3.1 Å resolution, respectively.

Expression, purification and preliminary structural analysis of Escherichia coli MatP in complex with the matS DNA site

D. Durand, I. Li de la Sierra-Gallay, M. A. Brooks, A. W. Thompson, N. Lazar, J. Lisboa, H. van Tilbeurgh and S. Quevillon-Cheruel

Structure of the regulatory domain of the LysR family regulator NMB2055 (MetR-like protein) from Neisseria meningitidis

S. Sainsbury, J. Ren, N. J. Saunders, D. I. Stuart and R. J. Owens

Synopsis: The crystal structure of the regulatory domain of NMB2055, a putative MetR regulator, was solved at 2.5 Å resolution.

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