Biological Crystallography

 

Acta Cryst. (2000). D56, 1359-1366  [ doi:10.1107/S0907444900009537 ]

Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis

C. J. Bond, J. Huang, R. Hajduk, K. E. Flick, P. J. Heath and B. L. Stoddard

Synopsis: The gene encoding iron superoxide dismutase from P. ovalis has been cloned and sequenced; the recombinant enzyme has been crystallized and its structure determined.

PDB reference: 1dt0

Online November 2000

 

Acta Cryst. (2000). D56, 1367-1375  [ doi:10.1107/S0907444900009896 ]

Structure of XynB, a highly thermostable -1,4-xylanase from Dictyoglomus thermophilum Rt46B.1, at 1.8  Å resolution

A. A. McCarthy, D. D. Morris, P. L. Bergquist and E. N. Baker

Synopsis: The structure of a highly thermostable family II xylanase has been determined at 1.8  Å resolution. Thermostability is attributed to increased polar surface and increased hydrogen bonding.

PDB reference: 1f5j

Online November 2000

 

Acta Cryst. (2000). D56, 1376-1384  [ doi:10.1107/S0907444900010647 ]

Structure of the C123S mutant of dienelactone hydrolase (DLH) bound with the PMS moiety of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF)

A. Robinson, K. J. Edwards, P. D. Carr, J. D. Barton, G. D. Ewart and D. L. Ollis

Synopsis: The structure of the DLH (C123S) with PMS bound indicates that the reason the enzyme is able to catalyse a wide range of dissimilar substrates is a consequence of its capacity to undergo a coordinated restructuring of the active site and associated regions of the molecule without compromising the stability of its tertiary fold.

PDB reference: 1ggv

Online November 2000

 

Acta Cryst. (2000). D56, 1385-1394  [ doi:10.1107/S0907444900010659 ]

Structures of recombinant native and E202Q mutant human acetylcholinesterase complexed with the snake-venom toxin fasciculin-II

G. Kryger, M. Harel, K. Giles, L. Toker, B. Velan, A. Lazar, C. Kronman, D. Barak, N. Ariel, A. Shafferman, I. Silman and J. L. Sussman

Synopsis: The structures of recombinant wild-type human acetylcholinesterase and of its E202Q mutant, as complexes with fasciculin-II, a `three-finger' snake neurotoxin, have been determined to 2.8 and 2.7  Å resolution, respectively. The overall structures of these complexes are similar to those of the Torpedo and mouse enzymes with fasciculin-II. Comparison of the wild-type and the mutant structures shows that removal of the charged group from the protein core, and its substitution by a neutral isosteric moiety, does not disrupt the functional architecture of the active center.

PDB references: 1b41 and 1f8u

Online November 2000

 

Acta Cryst. (2000). D56, 1395-1400  [ doi:10.1107/S0907444900010763 ]

Structure of human -thrombin complexed with RWJ-51438 at 1.7  Å: unusual perturbation of the 60A-60I insertion loop

R. Recacha, M. J. Costanzo, B. E. Maryanoff, M. Carson, L. DeLucas and D. Chattopadhyay

Synopsis: The three-dimensional structure of the ternary complex consisting of human -thrombin, hirugen and the active-site inhibitor RWJ-51438 has been determined at 1.7  Å resolution.

PDB reference: 1doj

Online November 2000

    

Acta Cryst. (2000). D56, 1401-1407  [ doi:10.1107/S0907444900011501 ]

The atomic resolution structure of bucandin, a novel toxin isolated from the Malayan krait, determined by direct methods

P. Kuhn, A. M. Deacon, S. Comoso, G. Rajaseger, R. M. Kini, I. Usón and P. R. Kolatkar

Synopsis: The structure of bucandin, a novel three-finger toxin, has been determined by direct methods. The full-matrix least-squares refinement to atomic resolution, with anisotropic temperature factors, converged to an R factor of 12.4%.

PDB reference: 1f94

Online November 2000

 

Acta Cryst. (2000). D56, 1408-1412  [ doi:10.1107/S0907444900010052 ]

Crystallographic studies of the interaction between the ferredoxin-NADP⁺ reductase and ferredoxin from the cyanobacterium Anabaena: looking for the elusive ferredoxin molecule

R. Morales, G. Kachalova, F. Vellieux, M.-H. Charon and M. Frey

Synopsis: The first structure of a complex between ferredoxin-NADP⁺ reductase and ferredoxin has been solved at 2.4  Å resolution by molecular replacement, anomalous dispersion and R_min search methods. The crystal was treated as a merohedral twin.

PDB reference: 1ewy

Online November 2000

 

Acta Cryst. (2000). D56, 1413-1420  [ doi:10.1107/S0907444900010039 ]

Single-wavelength anomalous diffraction phasing revisited

L. M. Rice, T. N. Earnest and A. T. Brunger

Synopsis: Analyzing the anomalous diffraction from seven different selenomethionine-labelled crystals, we show that the combination of single-wavelength anomalous-diffraction phasing and solvent flattening is sufficient to unambiguously determine most structures.

Online November 2000

 

Acta Cryst. (2000). D56, 1421-1429  [ doi:10.1107/S0907444900011227 ]

Evaluation of phase accuracy via topological and geometrical analysis of electron-density maps

C. Colovos, E. A. Toth and T. O. Yeates

Synopsis: A systematic analysis of local and global protein electron-density map properties is used to derive an empirical function to measure the average error in diffraction phase sets.

Online November 2000

 

Acta Cryst. (2000). D56, 1430-1431  [ doi:10.1107/S0907444900011203 ]

Evaporative microdialysis: an effective improvement in an established method of protein crystallization

C. Bunick, A. C. T. North and G. Stubbs

Synopsis: Slow evaporation of the reservoir in microdialysis can lead to a significant increase in the crystal size of proteins for which the crystallization region of supersaturation is particularly limited.

Online November 2000

 

Acta Cryst. (2000). D56, 1432-1433  [ doi:10.1107/S0907444900010064 ]

Crystallization and preliminary crystallographic study of an extremophile cytochrome c₄ from Thiobacillus ferrooxidans

C. Abergel, M. Bruschi, S. Chenivesse, S. Guasco, G. Leroy and M.-T. Guidici-Orticoni

Synopsis: The acidic cytochrome c₄ from T. ferrooxidans has been cloned, expressed, purified and crystallized. The MAD method using the four Fe per asymmetric unit is used to solve the protein structure.

Online November 2000

 

Acta Cryst. (2000). D56, 1434-1436  [ doi:10.1107/S0907444900009719 ]

Crystallization and preliminary X-ray diffraction analysis of a eumenine mastoparan toxin: a new class of mast-cell degranulating peptide in the wasp venom

F. Canduri, P. Delatorre, V. Fadel, C. C. B. Lorenzi, J. H. Pereira, J. R. Olivieri, J. Ruggiero Neto, K. Konno, M. S. Palma, T. Yamane and W. F. de Azevedo

Synopsis: The crystallization and preliminary X-ray analysis of a mastoparan peptide isolated from the venom of the solitary wasp A. flavomarginatum micado is reported.

Online November 2000

 

Acta Cryst. (2000). D56, 1437-1439  [ doi:10.1107/S0907444900009859 ]

Preliminary crystallographic studies of an extremely thermostable KDG aldolase from Sulfolobus solfataricus

E. J. Hendry, C. L. Buchanan, R. J. M. Russell, D. W. Hough, C. D. Reeve, M. J. Danson and G. L. Taylor

Synopsis: The growth of three crystal forms of 2-keto-3-deoxygluconate aldolase from the hyperthermophilic archaeon S. solfataricus is reported.

Online November 2000

 

Acta Cryst. (2000). D56, 1440-1442  [ doi:10.1107/S0907444900009884 ]

Crystallization and preliminary X-ray diffraction analysis of a recombinant cysteine-free mutant of crmA

M. Simonovic, P. G. W. Gettins and K. Volz

Synopsis: The first crystallization of a serpin that is either viral or a cysteine proteinase inhibitor is described.

Online November 2000

 

Acta Cryst. (2000). D56, 1443-1445  [ doi:10.1107/S090744490000994X ]

Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Bacillus megaterium IWG3

K. Yamamoto, M. Kusunoki, I. Urabe, S. Tabata and S. Osaki

Synopsis: Glucose dehydrogenase from B. megaterium IWG3 has been crystallized and 1.7  Å resolution data have been collected using a synchrotron-radiation source.

Online November 2000

 

Acta Cryst. (2000). D56, 1446-1448  [ doi:10.1107/S0907444900009951 ]

Crystallization and preliminary X-ray crystallographic studies of response regulator for cyanobacterial phytochrome, Rcp1

Y. J. Im, C.-M. Park, J.-I. Kim, S.-S. Yang, J.-G. Kang, S.-H. Rho, J. I. Kim, W. K. Song, P.-S. Song and S. H. Eom

Synopsis: The key response regulator of light regulation, Rcp1, from Synechocystis sp. has been crystallized in a form suitable for X-ray diffraction studies. The crystals belong to space group P6₃ and diffracted to 2.5  Å.

Online November 2000

 

Acta Cryst. (2000). D56, 1449-1451  [ doi:10.1107/S0907444900010027 ]

Expression, crystallization and preliminary X-ray diffraction studies of recombinant Bacillus anthracis lethal factor

L. Bernardi, G. Vitale, C. Montecucco and A. Musacchio

Synopsis: The lethal factor (LF) produced by toxigenic strains of B. anthracis is a Zn-endopeptidase responsible of the lethal effects of anthrax. LF has been expressed in E. coli as recombinant GST fusion, purified and crystallized; preliminary crystallographic studies are described.

Online November 2000

 

Acta Cryst. (2000). D56, 1452-1455  [ doi:10.1107/S0907444900010143 ]

Crystallization and preliminary X-ray diffraction analysis of a functional form of pneumolysin, a virulence factor from Streptococcus pneumoniae

S. J. Kelly and M. J. Jedrzejas

Synopsis: Crystals of a functional form of pneumolysin, a virulence factor of S. pneumoniae, have been obtained in complex with its activator, a cholesterol molecule, by the vapor-diffusion method. The crystallization process as well as the preliminary X-ray analysis are discussed.

Online November 2000

 

Acta Cryst. (2000). D56, 1456-1458  [ doi:10.1107/S0907444900010295 ]

Crystallization and preliminary X-ray diffraction studies on the DNA-binding domain of the multidrug transporter activation protein (MtaN) from Bacillus subtilis

M. H. Godsey, N. N. Baranova, A. A. Neyfakh and R. G. Brennan

Synopsis: The DNA-binding domain of the MerR family member MtaN has been crystallized in space group I2₁2₁2₁. Intensity data have been collected from these crystals at 100  K at a synchrotron-radiation source to 2.75  Å resolution.

Online November 2000

 

Acta Cryst. (2000). D56, 1459-1461  [ doi:10.1107/S0907444900010301 ]

Crystallization and preliminary X-ray analysis of full-length annexin I comprising the core and N-terminal domain

A. Rosengarth and H. Luecke

Synopsis: Annexin I, a member of the annexin family of Ca²⁺- and phospholipid-binding proteins, has been crystallized with the complete N-terminus.

Online November 2000

 

Acta Cryst. (2000). D56, 1462-1463  [ doi:10.1107/S0907444900010374 ]

Crystallization and preliminary X-ray analysis of a bacterial lysozyme produced by Streptomyces globisporus

T. Shiba, S. Harada, H. Sugawara, H. Naitow, Y. Kai and Y. Satow

Synopsis: A bacterial lysozyme produced by S. globisporus was crystallized in space group P4₁2₁2, with unit-cell parameters a = 63.11, c = 121.1  Å. A clear molecular-replacement solution was obtained and the structure refinement is in progress.

Online November 2000

 

Acta Cryst. (2000). D56, 1464-1465  [ doi:10.1107/S0907444900010386 ]

Expression, purification and crystallization of human tau-protein kinase I/glycogen synthase kinase-3

M. Aoki, M. Iwamoto-Sugai, I. Sugiura, C. Sasaki, T. Hasegawa, C. Okumura, S. Sugio, T. Kohno and T. Matsuzaki

Synopsis: Full-length human tau-protein kinase I (TPK-I; also known as glycogen synthase kinase-3, GSK-3) was produced in E. coli and crystallized. The crystals diffract up to 2.3  Å resolution and belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 82.9, b = 86.1, c = 178.1  Å measured at 100  K.

Online November 2000

 

Acta Cryst. (2000). D56, 1466-1467  [ doi:10.1107/S0907444900010660 ]

Crystallization and preliminary crystallographic studies of trichomaglin, a novel ribosome-inactivating protein

J. Wu, J.-H. Gan and Z.-X. Xia

Synopsis: Trichomaglin has been crystallized in two crystal forms.

Online November 2000

 

Acta Cryst. (2000). D56, 1468-1469  [ doi:10.1107/S0907444900010738 ]

Crystallization and preliminary X-ray analysis of CTLA-4 (CD152) membrane-external domain

C. Y. Chang, W. H. Fenderson, T. B. Lavoie, R. J. Peach, H. M. Einspahr and S. Sheriff

Synopsis: The membrane-external domain of the immune modulator CTLA-4 (CD152) has been crystallized; the crystals diffract to 2.7  Å resolution at synchrotron sources.

Online November 2000

 

Acta Cryst. (2000). D56, 1470-1472  [ doi:10.1107/S090744490001074X ]

Crystallization and preliminary X-ray crystallographic studies of the thermoactive pullulanase type I, hydrolyzing -1,6 glycosidic linkages, from Fervidobacterium pennivorans Ven5

J. H. G. Lebbink, C. Bertoldo, G. Tibbelin, J. T. Andersen, F. Duffner, G. Antranikian and R. Ladenstein

Synopsis: F. pennivorans Ven5 pullulanase type I was crystallized in the presence of the inhibitor -cyclodextrin. The crystals belong to space group P2₁2₁2₁, with unit-cell parameters a = 76.8, b = 96.2, c = 98.5  Å, diffract to 2.2  Å using synchrotron radiation and contain one monomer in the asymmetric unit.

Online November 2000

 

Acta Cryst. (2000). D56, 1473-1475  [ doi:10.1107/S0907444900010702 ]

Crystallization and preliminary X-ray crystallographic studies on the bacteriophage 6 RNA-dependent RNA polymerase

S. J. Butcher, E. V. Makeyev, J. M. Grimes, D. I. Stuart and D. H. Bamford

Synopsis: Native and selenomethionyl-labelled RNA-dependent RNA polymerase from bacteriophage 6 crystallizes in space groups P2₁ and P3₁/P3₂. Data have been collected for both space groups to better than 3  Å resolution.

Online November 2000

 

Acta Cryst. (2000). D56, 1476-1478  [ doi:10.1107/S0907444900010714 ]

Crystallization and preliminary X-ray analysis of the auxin receptor ABP1

E.-J. Woo, J. Bauly, J.-G. Chen, J. Marshall, H. Macdonald, C. Lazarus, P. Goodenough, M. Venis, R. Napier and R. Pickersgill

Synopsis: Monoclinic crystals of the maize auxin receptor ABP1 diffract to 1.9  Å resolution.

Online November 2000

 

Acta Cryst. (2000). D56, 1479-1481  [ doi:10.1107/S0907444900010957 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of human CCG1-interacting factor B

B. Padmanabhan, T. Kuzuhara, H. Mizuno and M. Horikoshi

Synopsis: Preliminary crystal data have been obtained for the first time for human CCG1-interacting factor B. The results of preliminary X-ray diffraction are discussed.

Online November 2000

 

Acta Cryst. (2000). D56, 1482-1484  [ doi:10.1107/S0907444900011136 ]

Crystallization and preliminary X-ray diffraction studies of a new crystal form of human secretory type IIA phospholipase A₂

W. B. Church, P.-W. Lei, D. J. Ogg and K. F. Scott

Synopsis: Preliminary crystal data have been obtained for a new crystal form of human secretory phospholipase A₂ IIA crystallized with pentapeptide inhibitors. The enzyme crystallizes in space group C2, with unit-cell parameters a = 140.8, b = 38.9, c = 109.1  Å, = 125.1°.

Online November 2000

 

Acta Cryst. (2000). D56, 1485-1487  [ doi:10.1107/S0907444900011240 ]

Nucleoside diphosphate kinase from the hyperthermophilic archaeon Methanococcus jannaschii: overexpression, crystallization and preliminary X-ray crystallographic analysis

K. Min, H. K. Song, C. Chang, J. Y. Lee, S. H. Eom, K. K. Kim, Y. G. Yu and S. W. Suh

Synopsis: Nucleoside diphosphate kinase from M. jannaschii has been crystallized using polyethylene glycol 4000 as precipitant. Native data have been collected to 2.30  Å resolution using synchrotron X-rays.

Online November 2000

 

Acta Cryst. (2000). D56, 1488-1491  [ doi:10.1107/S0907444900011306 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of ATP-phosphoribosyltransferase from Escherichia coli

B. Lohkamp, J. R. Coggins and A. J. Lapthorn

Synopsis: Crystals of ATP-phosphoribosyltransferase from E. coli were obtained by the vapour-diffusion method and diffract to 2.7  Å. The quaternary structure is determined based on the preliminary crystallographic studies.

Online November 2000

 

Acta Cryst. (2000). D56, 1492-1494  [ doi:10.1107/S0907444900011380 ]

Preliminary X-ray diffraction studies of rabbit muscle triose phosphate isomerase (TIM)

R. Aparicio, S. T. Ferreira, N. R. Leite and I. Polikarpov

Synopsis: Here the crystallization, data collection and molecular-replacement solutions obtained for two crystalline forms of triose phosphate isomerase, one determined at 85  K and the other at 298  K are reported.

Online November 2000

 

Acta Cryst. (2000). D56, 1495-1497  [ doi:10.1107/S0907444900011446 ]

Cloning, expression, purification and crystallization of dihydroxybutanone phosphate synthase from Magnaporthe grisea

D.-I. Liao, P. V. Viitanen and D. B. Jordan

Synopsis: Dihydroxybutanone phosphate synthase from M. grisea was cloned, expressed and crystallized. Crystals diffract to 1.0  Å resolution.

Online November 2000

 

Acta Cryst. (2000). D56, 1498-1500  [ doi:10.1107/S0907444900011744 ]

Crystallization and preliminary X-ray studies of oligandrin, a sterol-carrier elicitor from Pythium oligandrum

M.-B. Lascombe, M.-L. Milat, J.-P. Blein, F. Panabières, M. Ponchet and T. Prangé

Synopsis: Oligandrin, a small 10  kDa phytotoxin with sterol-carrier properties, has been crystallized from PEG 4000 at pH 4.5.

Online November 2000

 

Acta Cryst. (2000). D56, 1501-1504  [ doi:10.1107/S0907444900010040 ]

The purification, crystallization and preliminary structural characterization of glucose-1-phosphate thymidylyltransferase (RmlA), the first enzyme of the dTDP-L-rhamnose synthesis pathway from Pseudomonas aeruginosa

W. Blankenfeldt, M.-F. Giraud, G. Leonard, R. Rahim, C. Creuzenet, J. S. Lam and J. H. Naismith

Synopsis: The anabolism of L-rhamnose in many pathogenic bacteria presents itself as a possible novel therapeutic target. RmlA is the first of the four enzymes involved in this pathway. Crystallization and preliminary structural characterization are reported.

Online November 2000

 

Acta Cryst. (2000). D56, 1505-1507  [ doi:10.1107/S0907444900010076 ]

Crystallization and preliminary crystallographic studies of a new L-asparaginase encoded by the Escherichia coli genome

D. Borek and M. Jaskólski

Synopsis: A new E. coli L-asparaginase belonging to the class of Ntn hydrolases has been crystallized in the orthorhombic space group P2₁2₁2₁. The crystals diffract to 1.65  Å resolution and contain an ()₂ heterotetramer in the asymmetric unit, where the subunits and are the product of autocatalytic cleavage of the immature protein.

Online November 2000

 

Acta Cryst. (2000). D56, 1508-1509  [ doi:10.1107/S0907444900011756 ]

Purification, crystallization and preliminary diffraction study of -galactosidase from Penicillium sp.

K. N. Neustroev, E. A. de Sousa, A. M. Golubev, J. R. Brandão Neto, E. V. Eneyskaya, A. A. Kulminskaya and I. Polikarpov

Synopsis: -galactosidase from Penicillium sp. was crystallized and diffracted to 1.85  Å resolution. It belonged to the tetragonal P4₃(P4₁) space group, with unit-cell parameters a = b = 110.82, c = 161.28  Å.

Online November 2000

 

Acta Cryst. (2000). D56, 1510-1511  [ doi:10.1107/S0907444900010751 ]

Apotheosis, not apocalypse: methods in protein crystallography

V. S. Lamzin, A. Perrakis, G. Bricogne, J. Jiang, S. Swaminathan and J. L. Sussman

Synopsis: Report of the workshop Toward Automation of Structure Determination in Macromolecular Crystallography held on 20-21 June 1999.

Online November 2000

 

Acta Cryst. (2000). D56, 1512  [ doi:10.1107/S0907444900013640 ]

Crystallization and preliminary X-ray diffraction studies of monomeric isocitrate dehydrogenase from Corynrbacterium glutamicum. Erratum

G. F. Audette, J. W. Quail, K. Hayakawa, C. Bai, R. Chen and L. T. J. Delbaere

Synopsis: An erratum to Acta Cryst. (1999), D55, 1584-1585.

Online November 2000

 

Acta Cryst. (2000). D56, 1512  [ doi:10.1107/S0907444900014219 ]

Crystallization and preliminary X-ray analysis of the conserved domain IV of Escherichia coli 4.5S RNA. Erratum

L. Jovine, T. Hainzl, C. Oubridge and K. Nagai

Synopsis: An erratum to Acta Cryst. (2000), D56, 1033-1037.

Online November 2000