Biological Crystallography

 

Acta Cryst. (2001). D57, 767-774  [ doi:10.1107/S0907444901004255 ]

Atomic resolution structure of Escherichia coli dUTPase determined ab initio

A. González, G. Larsson, R. Persson and E. Cedergren-Zeppezauer

Synopsis: Derivatization with a mercury compound increases the diffraction limit of E. coli dUTPase crystals to atomic resolution. Data collection to 1.05  Å allows the structure to be solved from the position of the single Hg atom in the asymmetric unit. The final refined dUTPase derivative structure is described and compared with the native structure.

PDB references: 1eu5 and 1euw

Online 25 May 2001

 

Acta Cryst. (2001). D57, 775-783  [ doi:10.1107/S0907444901004243 ]

Avian haemoglobins and structural basis of high affinity for oxygen: structure of bar-headed goose aquomet haemoglobin

X.-Z. Liu, S.-L. Li, H. Jing, Y.-H. Liang, Z.-Q. Hua and G.-Y. Lu

Synopsis: The structure of bar-headed goose aquomet haemoglobin showed quaternary structural differences from lowland animal haemoglobins; critical residue differences are located in the ₁₁ interface.

PDB reference: 1c40

Online 25 May 2001

 

Acta Cryst. (2001). D57, 784-792  [ doi:10.1107/S0907444901004528 ]

Structure of C-phycocyanin from Spirulina platensis at 2.2  Å resolution: a novel monoclinic crystal form for phycobiliproteins in phycobilisomes

X.-Q. Wang, L.-N. Li, W.-R. Chang, J.-P. Zhang, L.-L. Gui, B.-J. Guo and D.-C. Liang

Synopsis: The monoclinic crystal structure of C-phycocyanin from S. platensis has been determined at 2.2  Å resolution. The unique side-by-side packing of two hexamers in the asymmetric unit makes it possible that lateral energy transfer takes place by the 155-155 route.

PDB reference: 1gh0

Online 25 May 2001

 

Acta Cryst. (2001). D57, 793-799  [ doi:10.1107/S0907444901004875 ]

Structure of the N-terminal domain of Yersinia pestis YopH at 2.0  Å resolution

A. G. Evdokimov, J. E. Tropea, K. M. Routzahn, T. D. Copeland and D. S. Waugh

Synopsis: The crystal structure of the N-terminal regulatory domain of the Y. pestis effector protein YopH has been determined at a resolution of 2.0  Å.

PDB reference: 1huf

Online 25 May 2001

 

Acta Cryst. (2001). D57, 800-805  [ doi:10.1107/S0907444901004887 ]

Solving a 300  kDa multimeric protein by low-resolution MAD phasing and averaging/phase extension

F. X. Gomis-Rüth and M. Coll

Synopsis: The structure of a homohexameric protein particle was solved by employing a tantalum bromide derivative of the crystals obtained. A low-resolution MAD experiment (three wavelengths) rendered phases that could be improved by averaging and extended by applying density modifications.

PDB references: 1e9r and 1e9s

Online 25 May 2001

 

Acta Cryst. (2001). D57, 806-812  [ doi:10.1107/S0907444901003523 ]

Stabilization of active-site loops in NH₃-dependent NAD⁺ synthetase from Bacillus subtilis

Y. Devedjiev, J. Symersky, R. Singh, M. Jedrzejas, C. Brouillette, W. Brouillette, D. Muccio, D. Chattopadhyay and L. DeLucas

Synopsis: The order/disorder changes of active-site loops upon substrate binding are described and implications for substrate recognition and stabilization are shown, along with the effect of a higher crystallization pH.

PDB references: 1ifx, 1fyd, 1ee1 and 1ih8

Online 25 May 2001

 

Acta Cryst. (2001). D57, 813-828  [ doi:10.1107/S0907444901003936 ]

Checking nucleic acid crystal structures

U. Das, S. Chen, M. Fuxreiter, A. A. Vaguine, J. Richelle, H. M. Berman and S. J. Wodak

Synopsis: The program SFCHECK, which analyses the quality of the structure-factor data, and the agreement of those data with the atomic coordinates, is used to survey 105 nucleic acid crystal structures from the NDB.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 829-839  [ doi:10.1107/S0907444901003791 ]

X-ray diffraction and atomic force microscopy analysis of twinned crystals: rhombohedral canavalin

T.-P. Ko, Y. G. Kuznetsov, A. J. Malkin, J. Day and A. McPherson

Synopsis: Hemihedrally twinned rhombohedral crystals of the plant seed protein canavalin were successfully refined. Their investigation by atomic force microscopy revealed an extensive defect structure that is likely to be a consequence of the twinning.

PDB references: 1dgw and 1dgr

Online 25 May 2001

 

Acta Cryst. (2001). D57, 840-846  [ doi:10.1107/S0907444901005832 ]

Laboratory multiple-crystal X-ray topography and reciprocal-space mapping of protein crystals: influence of impurities on crystal perfection

Z. W. Hu, B. R. Thomas and A. A. Chernov

Synopsis: Laboratory double-axis multiple-crystal X-ray topography and triple-axis high angular resolution reciprocal-space mapping have been combined to investigate the influence of acetylated lysozyme upon lysozyme crystal perfection.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 847-849  [ doi:10.1107/S0907444901004371 ]

Crystallization and preliminary crystallographic data of fructose-1,6-bisphosphatase from human muscle

D.-W. Zhu, G.-J. Xu, P. Rehse, R. Shi, F.-K. Zhao and S.-X. Lin

Synopsis: Crystallization of human muscle fructose-1,6-bisphosphatase has yielded complete data to 2.04  Å.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 850-853  [ doi:10.1107/S0907444901003900 ]

Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein-DNA complexes with involvement of AML1/Runx-1/CBF Runt domain, CBF and the C/EBP bZip region

T. H. Tahirov, T. Inoue-Bungo, M. Sasaki, M. Shiina, K. Kimura, K. Sato, T. Kumasaka, M. Yamamoto, N. Kamiya and K. Ogata

Synopsis: AML1/Runx-1/CBF(Runt)-CBF-C/EBP(bZip)-DNA, AML1/Runx-1/CBF(Runt)-C/EBP(bZip)-DNA and AML1/Runx-1/CBF(Runt)-DNA, three types of protein-DNA complexes, have been crystallized. The former two crystals diffracted to 3  Å resolution and the latter crystal diffracted to 2.65  Å resolution.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 854-856  [ doi:10.1107/S0907444901003912 ]

Crystallization and preliminary X-ray analysis of the C/EBP C-terminal region in complex with DNA

T. H. Tahirov, T. Inoue-Bungo, M. Sasaki, A. Fujikawa, K. Kimura, K. Sato, S. Adachi, N. Kamiya and K. Ogata

Synopsis: Two kinds of C-terminal fragments of human C/EBP (residues 259-345 and 259-336) were crystallized in complex with a 16 bp DNA fragment from the tom-1 promoter. These two types of crystals diffracted to 2.1 and 1.8  Å resolution, respectively.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 857-859  [ doi:10.1107/S0907444901004012 ]

Crystallization and preliminary X-ray studies of meso-2,3-butanediol dehydrogenase from Klebsiella pneumoniae IAM1063

M. Otagiri, G. Kurisu, S. Swaminathan, S. Ui, S. Yoneda, M. Ohkuma, T. Kudo and M. Kusunoki

Synopsis: Two different crystal forms of meso-2,3-butanediol dehydrogenase from K. pneumoniae IAM1063 have been obtained. Crystal forms I and II belong to space groups C2 and P2₁ and diffract to 2.0 and 1.7  Å resolution, respectively.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 860-861  [ doi:10.1107/S0907444901004437 ]

Crystallization and preliminary X-ray study of haem-binding protein from the bloodsucking insect Rhodnius prolixus

R. A. P. Nagem, J. R. Brandão Neto, V. P. Forrer, M. H. Sorgine, G. O. Paiva-Silva, H. Masuda, R. Meneghini, P. L. Oliveira and I. Polikarpov

Synopsis: Rhodnius haem-binding protein (RHBP) from the bloodsucking insect R. prolixus, a 15  kDa protein, has been crystallized using polyethylene glycol as a precipitant. The crystals belong to the space group P4₁₍₃₎2₁2 and diffract beyond 2.6  Å resolution.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 862-864  [ doi:10.1107/S0907444901004553 ]

Two different crystal forms of sorcin, a penta-EF-hand Ca²⁺-binding protein

V. Nastopoulos, A. Ilari, G. Colotti, C. Zamparelli, D. Verzili, E. Chiancone and D. Tsernoglou

Synopsis: Sorcin, a penta EF-hand Ca²⁺-binding protein, and its Ca²⁺-binding domain have been crystallized. The crystals of the entire molecule diffract poorly, but two crystal forms grown from the domain that lacks the first 32 amino acids diffract to 2.1 and 2.7  Å resolution.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 865-866  [ doi:10.1107/S0907444901004607 ]

Crystallization and preliminary crystallographic studies of a ribosomal protein L30e from the hyperthermophilic archaeon Thermococcus celer

K.-B. Wong, W. K. Wang, M. R. Proctor, M. Bycroft and Y. W. Chen

Synopsis: Crystals of ribosomal protein L30e from the hyperthermophilic archaeon T. celer were obtained and data diffracting to 1.96  Å were collected using a Cu  K X-ray source. The crystals belong to space group P6₁/P6₅, with unit-cell parameters a = b = 48.32, c = 86.42  Å.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 867-869  [ doi:10.1107/S0907444901004619 ]

Optimization of Met8p crystals through protein-storage buffer manipulation

H. L. Schubert, E. Raux, M. J. Warren and K. S. Wilson

Synopsis: Met8p was successfully crystallized by combining crystallization screening results with incremental adjustments to the protein storage buffer.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 870-872  [ doi:10.1107/S0907444901004929 ]

Crystallization of two glutathione S-transferases from an unusual gene family

A. J. Oakley, K. Jirajaroenrat, T. Harnnoi, A. J. Ketterman and M. Wilce

Synopsis: Two glutathione S-transferase isozymes from the mosquito Anopheles dirus (AdGST1-3 and AdGST1-4) from an alternately spliced gene family have been expressed, purified and crystallized. Determination of these structures will advance our understanding of how these enzymes inactivate pesticides and the structural consequences of alternate splicing.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 873-875  [ doi:10.1107/S0907444901005054 ]

Crystallization and preliminary investigations on a telomeric repeat sequence C₄A₂C₄A₂

G. Savitha, D. Leonidas, K. R. Acharya and M. A. Viswamitra

Synopsis: The space group and the unit-cell parameters of crystals of C₂A₂C₄A₂ and Patterson and molecular-replacement studies on the crystals suggest that the molecules assume a tetraplex structure.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 876-878  [ doi:10.1107/S0907444901007727 ]

Crystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis

R. Persson, M. Harkiolaki, J. McGeehan and K. S. Wilson

Synopsis: Four different crystal forms of B. subtilis dUTPase in unliganded and potentially liganded (with a substrate analogue) forms have been analysed using synchrotron radiation.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 879-881  [ doi:10.1107/S0907444901005285 ]

Crystallization and preliminary X-ray diffraction analysis of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase of Synechococcus PCC 7942

Y. Nakamura, T. Tada, K. Wada, T. Kinoshita, M. Tamoi, S. Shigeoka and K. Nishimura

Synopsis: NADP-dependent glyceraldehyde-3-phosphate dehydrogenase of Synechococcus PCC 7942 was crystallized in two different forms using ammonium sulfate as a precipitant. Form I crystals belong to the hexagonal space group P6₅ and form II crystals belong to the monoclinic space group C2.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 882-883  [ doi:10.1107/S0907444901005273 ]

Preliminary X-ray crystallographic analysis of a Ca²⁺-binding protein human S100A1

Z. Wang, H. Zhang, Y. Ding, G. Wang, X. Wang, S. Ye, M. Bartlam, H. Tang, Y. Liu, F. Jiang, R. Barraclough, P. S. Rudland and Z. Rao

Synopsis: S100A1, a Ca²⁺-binding protein from the S100 protein family, has been crystallized. The crystals were stable during exposure to X-rays and diffracted to 2.6  Å resolution in-house.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 884-885  [ doi:10.1107/S090744490100525X ]

Crystallization and preliminary X-ray analysis of AlgS, a bacterial ATP-binding cassette (ABC) protein specific to macromolecule import

Y. Mishima, K. Momma, W. Hashimoto, B. Mikami and K. Murata

Synopsis: Hexahistidine-tagged AlgS of Sphingomonas sp. A1 was overexpressed in E. coli and crystallized. X-ray diffraction data to 3.2  Å have been collected from the native crystal.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 886-888  [ doi:10.1107/S0907444901005261 ]

Crystallization and quaternary structure analysis of an Lrp-like regulatory protein from the hyperthermophile Pyrococcus furiosus

S. E. Sedelnikova, S. H. J. Smits, P. M. Leonard, A. B. Brinkman, J. van der Oost, J. B. Rafferty and D. W. Rice

Synopsis: The Lrp-like regulatory protein, LrpA, from P. furiosus has been crystallized in a form suitable for X-ray diffraction studies. An octameric assembly for the protein is suggested by its behaviour on gel-filtration columns and analysis of the self-rotation function.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 889-890  [ doi:10.1107/S090744490100556X ]

Crystallization and preliminary crystallographic studies of RhoGDI in complex with the radixin FERM domain

K. Hamada, A. Seto, T. Shimizu, T. Matsui, Y. Takai, S. Tsukita, S. Tsukita and T. Hakoshima

Synopsis: Crystals of RhoGDI complexed with the FERM domain of radixin were obtained and belong to the space group P2₁2₁2, with unit-cell parameters a = 130.9, b = 151.2, c = 71.2  Å. Diffraction data to a resolution of 2.9  Å have been obtained using synchrotron radiation.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 891-892  [ doi:10.1107/S0907444901005716 ]

Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail of the adhesion protein ICAM-2

K. Hamada, T. Shimizu, T. Matsui, S. Tsukita, S. Tsukita and T. Hakoshima

Synopsis: Crystals of the complex between the FERM domain of radixin and the cytoplasmic tail of ICAM-2 were obtained. The binary complex crystals were found to belong to the space group P3₁21 or P3₂21, with unit-cell parameters a = b = 100.44, c = 99.49  Å, and diffract to 2.6  Å resolution.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 893-895  [ doi:10.1107/S0907444901005686 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the yeast Sec12p protein, a guanine nucleotide-exchange factor involved in vesicle transport

L. Dumon-Seignovert, T. Matsumuto, S. Monaco-Malbet, T. Tomizaki, M. Sato, K. Sato, A. Nakano and S. Wakatsuki

Synopsis: A 38.5  kDa cytoplasmic domain of Sec12p from S. cerevisiae, a guanine nucleotide-exchange factor of a GTP-binding protein Sar1p, a key factor in vesicle transport from the endoplasmic reticulum to the Golgi apparatus, has been expressed in S. cerevisiae and in E. coli. The proteins have been purified to homogeneity and X-ray diffraction data sets have been collected from two crystal forms to 2.6 and 2.0  Å resolution.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 896-897  [ doi:10.1107/S0907444901005728 ]

The MinD protein from the hyperthermophilic archaeon Pyrococcus horikoshii: crystallization and preliminary X-ray analysis

N. Sakai, H. Itou, N. Watanabe, M. Yao and I. Tanaka

Synopsis: The cell-division inhibitor MinD from P. horikoshii was crystallized and preliminary X-ray diffraction studies were performed.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 898-899  [ doi:10.1107/S0907444901005777 ]

Crystallization and preliminary X-ray analysis of the complex between human CTLA-4 and B7-2

X. Zhang, J.-C.D. Schwartz, S. G. Nathenson and S. C. Almo

Synopsis: The receptor-binding domain of human B7-2 and the disulfide-linked homodimer of human CTLA-4 have been produced in E. coli, purified and cocrystallized in a monoclinic form (space group P2₁). A single crystal was obtained that diffracted to 3.2  Å resolution.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 900-905  [ doi:10.1107/S0907444901005820 ]

Crystallization of a recombinant form of the complete sequence of human -interferon: characterization by small-angle X-ray scattering, mass spectrometry and preliminary X-ray diffraction studies

M. Budayova-Spano, W. Shepard, B. Schoot, J.-P. Astier and S. Veesler

Synopsis: The crystallization conditions of a recombinant form of the complete sequence of human -interferon have been determined after studying the behaviour of this protein in solution by small-angle X-ray scattering (SAXS) as a function of pH and salt type.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 906-908  [ doi:10.1107/S0907444901006539 ]

Crystallization and preliminary crystallographic characterization of catechol-O-methyltransferase in complex with its cosubstrate and an inhibitor

M. L. Rodrigues, M. Archer, M. J. Bonifácio, P. Soares-da-Silva and M. A. Carrondo

Synopsis: Recombinant catechol-O-methyltransferase has been crystallized with a new tight-binding inhibitor. This enzyme is an important target in the treatment of Parkinson's disease.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 909-911  [ doi:10.1107/S0907444901007296 ]

Cloning, expression, purification and preliminary X-ray crystallographic studies of Escherichia coli Hsp100 ClpB nucleotide-binding domain 1 (NBD1)

J. Li and B. Sha

Synopsis: The E. coli Hsp100 ClpB nucleotide-binding domain 1 (NBD1) has been cloned, purified and crystallized. The crystals diffracted to 1.8  Å.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 912-914  [ doi:10.1107/S0907444901004620 ]

Crystallization and preliminary X-ray studies of snake gourd lectin: homology with type II ribosome-inactivating proteins

N. Manoj, A. A. Jeyaprakash, J. V. Pratap, S. S. Komath, R. Kenoth, M. J. Swamy and M. Vijayan

Synopsis: Snake gourd seed lectin has been crystallized. Preliminary X-ray studies indicate the lectin to be homologous to type II ribosome-inactivating proteins.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 915-917  [ doi:10.1107/S0907444901004905 ]

New crystal forms of Trypanosoma cruzi dUTPase

M. Harkiolaki, A. M. Brzozowski, D. Gonzalez-Pacanowska, F. Hidalgo-Zarco and K. S. Wilson

Synopsis: Crystals of T. cruzi dUTPase in two crystals forms, both belonging to the hexagonal space group P6₃22, have been obtained. Form I is similar to crystals reported previously, but the crystals are of better quality and produced under different conditions, while form II has not been reported before.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 918-920  [ doi:10.1107/S0907444901005212 ]

Crystallization and preliminary X-ray diffraction studies of glycerol 3-phosphate cytidylyltransferase from Staphylococcus aureus

V. C. N. Yim, M. Zolli, D. S. Badurina, L. Rossi, E. D. Brown and A. M. Berghuis

Synopsis: Glycerol 3-phosphate cytidylyltransferase from S. aureus has been cloned, expressed, purified and subsequently crystallized. Determination of crystallization conditions required a combination of three optimization strategies.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 921-924  [ doi:10.1107/S0907444901004516 ]

Subunit dissociation and reassociation leads to preferential crystallization of haemoglobin Bart's (₄) from solutions of human embryonic haemoglobin Portland (₂₂) at low pH

R. D. Kidd, A. Mathews, H. M. Baker, T. Brittain and E. N. Baker

Synopsis: Low-pH incubation of human embryonic haemoglobin Portland (₂₂) has resulted in the preferential crystallization of haemoglobin Bart's (₄), as the result of dissociation of the embryonic heterotetramer and preferential formation of the homotetramer.

Online 25 May 2001

 

Acta Cryst. (2001). D57, 925-927  [ doi:10.1107/S090744490100378X ]

Amphiphiles modify the properties of detergent solutions used in crystallization of membrane proteins

M. A. Rosenow, J. C. Williams and J. P. Allen

Synopsis: The effect of the amphiphile heptanetriol on the critical micelle concentration and the cloud-point temperature was measured for several detergents commonly employed in the crystallization of membrane proteins.

Online 25 May 2001