Biological Crystallography

 

Acta Cryst. (2001). D57, 929-940  [ doi:10.1107/S0907444901004504 ]

Structural effects of monovalent anions on polymorphic lysozyme crystals

M. C. Vaney, I. Broutin, P. Retailleau, A. Douangamath, S. Lafont, C. Hamiaux, T. Prangé, A. Ducruix and M. Riès-Kautt

Synopsis: Anion sites in lysozyme polymorphic structures were classified as sequence-dependent, packing-dependent and unique non-specific sites.

PDB references: 1b0d, 1b2k, 1hf4 and 1lcn

Online 21 June 2001

 

Acta Cryst. (2001). D57, 941-947  [ doi:10.1107/S0907444901005625 ]

Intermonomer interactions in dimer of bovine heart cytochrome c oxidase

S. J. Lee, E. Yamashita, T. Abe, Y. Fukumoto, T. Tsukihara, K. Shinzawa-Itoh, H. Ueda and S. Yoshikawa

Synopsis: Nuclear-coded subunits stabilize a stable dimeric state in bovine heart cytochrome c oxidase.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 948-956  [ doi:10.1107/S0907444901005972 ]

Structure of aspergillopepsin I from Aspergillus phoenicis: variations of the S1'-S2 subsite in aspartic proteinases

S. W. Cho, N. Kim, M.-U. Choi and W. Shin

Synopsis: The crystal structure of aspergillopepsin I from A. phoenicis has been determined at 2.18  Å resolution. In the combined S1'-S2 subsite, aspergillopepsin I is similar to endothiapepsin but differs from penicillopepsin, which shares the higher sequence identity.

PDB reference: 1ibq

Online 21 June 2001

 

Acta Cryst. (2001). D57, 957-967  [ doi:10.1107/S0907444901006047 ]

Structure of a new polymorphic monoclinic form of human transthyretin at 3  Å resolution reveals a mixed complex between unliganded and T₄-bound tetramers of TTR

A. Wojtczak, P. Neumann and V. Cody

Synopsis: A new polymorphic form of human transthyretin contains a unique assembly of apo TTR and TTR-T₄ complex in the asymmetric unit of the monoclinic lattice. These data provide insight into the mechanism of negative cooperativity in T₄ binding.

PDB reference: 1ict

Online 21 June 2001

 

Acta Cryst. (2001). D57, 968-976  [ doi:10.1107/S0907444901006291 ]

Structure of ribosomal protein TL5 complexed with RNA provides new insights into the CTC family of stress proteins

R. Fedorov, V. Meshcheryakov, G. Gongadze, N. Fomenkova, N. Nevskaya, M. Selmer, M. Laurberg, O. Kristensen, S. Al-Karadaghi, A. Liljas, M. Garber and S. Nikonov

Synopsis: The crystal structure of ribosomal protein TL5 is the first structure of a full-size protein from the CTC family of stress proteins. Structure of TL5 complexed with RNA reveals new features of protein-RNA binding.

PDB reference: 1feu

Online 21 June 2001

 

Acta Cryst. (2001). D57, 977-980  [ doi:10.1107/S0907444901007314 ]

De novo structure determination of vancomycin aglycon using the anomalous scattering of chlorine

P. J. Loll

Synopsis: The anomalous scattering of chlorine has been exploited to determine the structure of the macromolecular antibiotic vancomycin aglycon.

PDB reference: 1ghg

Online 21 June 2001

 

Acta Cryst. (2001). D57, 981-989  [ doi:10.1107/S0907444901007120 ]

High-resolution neutron structure of nicotinamide adenine dinucleotide

B. Guillot, C. Lecomte, A. Cousson, C. Scherf and C. Jelsch

Synopsis: The crystal structure of NAD⁺ was determined by a neutron-diffraction experiment. The stereochemistry, hydrogen bonds and C-HO interactions are described and compared with several high-resolution NAD⁺ holoenzyme structures.

NDB reference: ur0013

Online 21 June 2001

 

Acta Cryst. (2001). D57, 990-995  [ doi:10.1107/S0907444901006382 ]

Anomalous signal of phosphorus used for phasing DNA oligomer: importance of data redundancy

Z. Dauter and D. A. Adamiak

Synopsis: The crystal structure of a left-handed Z-DNA duplex has been solved based on the anomalous signal of the P atoms with single-wavelength data collected at 1.54  Å. The redundancy of measurements crucially affects the phasing process.

PDB reference: 1ick

Online 21 June 2001

 

Acta Cryst. (2001). D57, 996-1002  [ doi:10.1107/S0907444901007260 ]

Protein crystal structure solution by fast incorporation of negatively and positively charged anomalous scatterers

R. A. P. Nagem, Z. Dauter and I. Polikarpov

Synopsis: The use of two different classes of compounds (monovalent and polyvalent cations) in the quick cryosoaking procedure for derivatization and phasing of protein crystals has been proposed. This approach was tested on hen egg-white lysozyme and was successfully employed to solve the structure of a novel protein.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1003-1007  [ doi:10.1107/S0907444901006370 ]

The absorption edge of protein-bound mercury and a double-edge strategy for HgMAD data acquisition

X. Ji, J. Blaszczyk and X. Chen

Synopsis: A double-edge approach with a set of four wavelengths [1.0087  Å (12  291  eV, edge1), 1.0084  Å (12  295  eV, edge2), 1.0064  Å (12  320  eV, peak) and 0.9918  Å (12  500  eV, remote)] is recommended for HgMAD data acquisition.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1008-1012  [ doi:10.1107/S0907444901007302 ]

Using surface-bound rubidium ions for protein phasing

S. Korolev, I. Dementieva, R. Sanishvili, W. Minor, Z. Otwinowski and A. Joachimiak

Synopsis: The anomalous signal from rubidium ions bound to the protein surface was used for phasing of the crystal structure of the hsp60 apical domain from T. thermophilus. Phase quality was sufficient for autotracing of the protein model at 2.0  Å resolution.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1013-1019  [ doi:10.1107/S0907444901006394 ]

A new software routine that automates the fitting of protein X-ray crystallographic electron-density maps

D. G. Levitt

Synopsis: This routine automates the steps that are usually followed by an investigator working at a graphics terminal. It is applicable to relatively low resolution maps (2.5 to 2.8  Å).

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1020-1029  [ doi:10.1107/S0907444901006242 ]

Effects of PEG on detergent micelles: implications for the crystallization of integral membrane proteins

C. Hitscherich, V. Aseyev, J. Wiencek and P. J. Loll

Synopsis: PEG is shown to promote micelle-micelle attraction and increase apparent micelle size, suggesting two mechanisms by which detergent effects can modulate the crystallization of integral membrane proteins.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1030-1031  [ doi:10.1107/S0907444901005571 ]

Preliminary crystallographic study of Thermus aquaticus glycerol kinase

H.-S. Huang, T. Inoue, K. Ito and T. Yoshimoto

Synopsis: Crystals of T. aquaticus GlpK have been obtained by the hanging-drop method. The crystals diffract to 2.87  Å resolution and belong to space group I23.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1032-1035  [ doi:10.1107/S0907444901006552 ]

Crystallization and preliminary X-ray analysis of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Staphylococcus aureus

M. S. Harris, J. T. Herberg, J. I. Cialdella, J. P. Martin, T. E. Benson, G. H. Choi and E. T. Baldwin

Synopsis: Diffraction-quality crystals of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from S. aureus have been prepared. The crystals belong to the cubic space group I2₁3, with unit-cell parameters a = b = c = 178.99  Å.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1036-1037  [ doi:10.1107/S0907444901007090 ]

Crystallization and X-ray diffraction measurements of a thermophilic archaeal recombinant amidase from Sulfolobus solfataricus MT4

V. Nastopoulos, B. Vallone, L. Politi, A. Scotto d'Abusco, R. Scandurra and D. Tsernoglou

Synopsis: A recombinant thermophilic archaeal amidase from S. solfataricus MT4 that catalyzes the hydrolysis of aliphatic and aromatic amides has been crystallized. Diffraction data for the native protein (to 2.55  Å) and a putative derivative (to 2.20  Å) have been collected.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1038-1041  [ doi:10.1107/S0907444901007272 ]

Cloning, purification, crystallization and preliminary X-ray diffraction analysis of the antistasin-type inhibitor ghilanten (domain I) from Haementeria ghilianii in complex with porcine -trypsin

U. Rester, W. Bode, C. A. M. Sampaio, E. Auerswald and A. P. Y. Lopes

Synopsis: The synthetic gene of the amino-terminal domain of the antistasin-type inhibitor ghilanten (ghilanten-D1) from H. ghilianii was constructed, expressed in the methylotrophic yeast P. pastoris and purified by heparin-Sepharose chromatography. Recombinant ghilanten-D1 has been crystallized in complex with porcine -trypsin in three different-looking but isomorphous crystal forms, each belonging to the orthorhombic space group P2₁2₁2₁.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1042-1045  [ doi:10.1107/S0907444901007284 ]

Characterization and crystallization of a novel Sarcocystis muris lectin, SML-2

J. J. Müller, E.-C. Müller, T. Montag, N. Zyto, B. Löschner, H. Klein, U. Heineman and A. Otto

Synopsis: A novel lectin SML-2 consisting of 138 amino acids was isolated from cyst merozoites of S. muris and sequenced by Edman degradation and mass spectrometry. Crystals diffracting to 2.1  Å resolution at a synchrotron were grown by the hanging-drop vapour-diffusion technique.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1046-1047  [ doi:10.1107/S0907444901007740 ]

Crystallization and preliminary X-ray crystallographic studies on 4--glucanotransferase from Thermotoga maritima

A. Roujeinikova, C. Raasch, S. Sedelnikova, W. Liebl and D. W. Rice

Synopsis: 4--Glucanotransferase from the hyperthermophilic bacterium T. maritima has been crystallized. The crystals belong to an I-centered orthorhombic space group and diffract to at least 2.6  Å resolution.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1048-1051  [ doi:10.1107/S0907444901005881 ]

An improved phase-extension procedure for isomorphous replacement phases

Y. Vekhter and R. Miller

Synopsis: A new phase-extension with density modification procedure to improve isomorphous replacement phases is described.

Online 21 June 2001

 

Acta Cryst. (2001). D57, 1052  [ doi:10.1107/S090744490100645X ]

Oxford handbook of nucleic acid structure

Online 21 June 2001