Biological Crystallography

 

Acta Cryst. (2001). D57, 1491-1497  [ doi:10.1107/S0907444901008897 ]

Structure of mouse L-chain ferritin at 1.6  Å resolution

T. Granier, B. Gallois, B. Langlois d'Estaintot, A. Dautant, J.-M. Chevalier, J.-M. Mellado, C. Beaumont, P. Santambrogio, P. Arosio and G. Precigoux

Synopsis: The mouse L-chain ferritin structure has been refined to 1.6  Å resolution from a cubic F432 crystal.

PDB reference: 1h96

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1498-1505  [ doi:10.1107/S0907444901010423 ]

Structure of cytochrome c₂ from Rhodospirillum centenum

A. Camara-Artigas, J. C. Williams and J. P. Allen

Synopsis: The structure of cytochrome c₂ from R. centenum was determined using X-ray diffraction at a resolution limit of 1.7  Å. The implications of the structural features, including a unique extended -helix and an unusual charge distribution on the surface, are discussed.

PDB reference: 1jdl

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1506-1512  [ doi:10.1107/S0907444901011143 ]

Structures of porcine -trypsin-detergent complexes: the stabilization of proteins through hydrophilic binding of polydocanol

S. Deepthi, A. Johnson and V. Pattabhi

Synopsis: Polydocanol binds to the hydrophilic regions on the surface of trypsin offering additional stability to the protein.

PDB references: 1fni, 1fmg and 1fn6

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1513-1517  [ doi:10.1107/S090744490101232X ]

Structure of Urtica dioica agglutinin isolectin I: dimer formation mediated by two zinc ions bound at the sugar-binding site

K. Harata, W.-D. Schubert and M. Muraki

Synopsis: The crystal structure of U. dioica agglutinin isolectin I was solved by molecular replacement. Two molecules form a pseudosymmetric dimer, mediated by two zinc ions that are bound by residues of the sugar-binding site of the C-terminal domain.

PDB reference: 1iqb

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1518-1525  [ doi:10.1107/S0907444901012604 ]

Structure of HisF, a histidine biosynthetic protein from Pyrobaculum aerophilum

M. J. Banfield, J. S. Lott, V. L. Arcus, A. A. McCarthy and E. N. Baker

Synopsis: The structure of the histidine biosynthetic enzyme HisF from the hyperthermophilic archaeon P. aerophilum has been determined at 2.0  Å resolution. Two tightly bound phosphate ions and two glycerol molecules mark out the probable binding site for the bisphosphoryl substrate of HisF.

PDB reference: 1h5y

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1526-1533  [ doi:10.1107/S0907444901012719 ]

The structure of human liver fructose-1,6-bisphosphate aldolase

A. R. Dalby, D. R. Tolan and J. A. Littlechild

Synopsis: The X-ray crystallographic structure determination of the human liver isozyme of fructose-1,6-bisphosphate aldolase by molecular replacement using a tetramer of the human muscle isozyme as a search model is described.

PDB reference: 1qo5

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1534-1544  [ doi:10.1107/S090744490101321X ]

Structure at 2.6  Å resolution of phenylalanyl-tRNA synthetase complexed with phenylalanyl-adenylate in the presence of manganese

R. Fishman, V. Ankilova, N. Moor and M. Safro

Synopsis: The crystal structure of phenylalanyl-tRNA synthetase from Thermus thermophilus, a class II aminoacyl-tRNA synthetase, complexed with phenylalanyl-adenylate has been determined at 2.6  Å resolution.

PDB reference: 1jjc

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1545-1551  [ doi:10.1107/S090744490101352X ]

Structure of murine Tcl1 at 2.5  Å resolution and implications for the TCL oncogene family

J. M. Petock, I. Y. Torshin, Y.-F. Wang, G. C. Du Bois, C. M. Croce, R. W. Harrison and I. T. Weber

Synopsis: The crystal structure of the murine Tcl1 dimer has been determined at 2.5  Å resolution with an R factor of 0.225. Structural comparisons of the Tcl1 oncoprotein family reveal a high degree of structural conservation, internal symmetry and a common planar surface likely to be involved in protein-protein interactions.

PDB reference: 1jnp

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1552-1559  [ doi:10.1107/S0907444901013543 ]

Structure of the neurotoxic complex vipoxin at 1.4  Å resolution

S. Banumathi, K. R. Rajashankar, C. Nötzel, B. Aleksiev, T. P. Singh, N. Genov and Ch. Betzel

Synopsis: The crystal structure of vipoxin, a neurotoxic postsynaptic heterodimeric complex from the venom of Vipera ammodytes meridionalis, has been determined by the molecular-replacement method and refined to 1.4  Å resolution.

PDB reference: 1jlt

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1560-1570  [ doi:10.1107/S0907444901013865 ]

Structure of human uropepsin at 2.45  Å resolution

F. Canduri, L. G. V. L. Teodoro, V. Fadel, C. C. B. Lorenzi, V. Hial, R. A. S. Gomes, J. R. Neto and W. F. de Azevedo

Synopsis: The crystal structure of uropepsin isolated from human urine is reported.

PDB reference: 1flh

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1571-1578  [ doi:10.1107/S090744490101335X ]

A quick solution: ab initio structure determination of a 19  kDa metalloproteinase using ACORN

K. E. McAuley, Y. Jia-Xing, E. J. Dodson, J. Lehmbeck, P. Østergaard and K. S. Wilson

Synopsis: The structure of an unknown metalloproteinase has been solved with the new direct-methods program ACORN using 1.0  Å resolution data. The whole process, starting from the processed data and ending with a refined model, required less than 6  h of computational time.

PDB reference: 1eb6

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1579-1583  [ doi:10.1107/S0907444901011209 ]

A new conformation of the integrin-binding fragment of human VCAM-1 crystallizes in a highly hydrated packing arrangement

P. Taylor, M. Bilsland and M. D. Walkinshaw

Synopsis: The X-ray crystal structure of two N-terminal integrin-binding IgSF domains of human vascular cell adhesion molecule (VCAM-1) shows an unusual and highly hydrated packing arrangement in which over 80% of the crystal is occupied by solvent. The conformation is also different, with a tilt angle between the two IgSF domains of 19.4°.

PDB reference: 1ij9

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1584-1594  [ doi:10.1107/S0907444901011957 ]

The combination of molecular dynamics with crystallography for elucidating protein-ligand interactions: a case study involving peanut lectin complexes with T-antigen and lactose

J. V. Pratap, G. M. Bradbrook, G. B. Reddy, A. Surolia, J. Raftery, J. R. Helliwell and M. Vijayan

Synopsis: Eight molecular-dynamics simulations, based on the crystal structures of PNA complexes with two sugars at 293 and 313  K, confirm the qualitative explanation for improved binding of one sugar over the other and provide a rationale for the temperature dependence of thermodynamic parameters and for the observed sugar-binding affinity of one of two available mutants. They also demonstrate the usefulness of carrying out several simulations with different starting conditions. The results of the work may have implications for rational drug-design approaches.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1595-1608  [ doi:10.1107/S090744490101215X ]

High-resolution experimental phases for tryptophanyl-tRNA synthetase (TrpRS) complexed with tryptophanyl-5'AMP

P. Retailleau, Y. Yin, M. Hu, J. Roach, G. Bricogne, C. Vonrhein, P. Roversi, E. Blanc, R. M. Sweet and C. W. Carter

Synopsis: Independent SAD, MAD and MIRAS 1.7  Å phase sets for B. stearothermophilus tryptophanyl-tRNA synthetase show that SAD phasing compares very favourably with the other methods. Maximum-likelihood refinements of the structure against the three sets of experimental amplitudes and phases agree closely, providing significant structural revisions throughout the former 2.9  Å model and documenting subtle localized structural differences between native and selenomethionylated protein.

PDB references: 1i6m, 1i6l and 1i6k

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1609-1613  [ doi:10.1107/S0907444901012318 ]

Destabilizing effect of a fluorouracil extra base in a hybrid RNA duplex compared with bromo and chloro analogues

W. Cruse, P. Saludjian, A. Neuman and T. Prangé

Synopsis: The destabilizing effect of fluorouracil is compared with the effect of chloro- and bromouracil when they are introduced into a short RNA sequence.

PDB references: 1idw, 1iha, 1id9 and 1icg#

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1614-1620  [ doi:10.1107/S090744490101280X ]

The effect of stabilizing additives on the structure and hydration of proteins: a study involving tetragonal lysozyme

S. Datta, B. K. Biswal and M. Vijayan

Synopsis: A comparison involving the structures of tetragonal lysozyme grown in the presence of sucrose, sorbitol and trehalose, and those of the orthorhombic and the monoclinic crystals grown under conditions in which tetragonal lysozyme is grown shows that the effect of the additives on the structure and, more importantly, the hydration shell of the protein is less than the normal effect of changes in molecular packing.

PDB references: 1jis, 1jjo, 1jiy, 1jit, 1jj1 and 1jj3

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1621-1629  [ doi:10.1107/S0907444901013610 ]

Systematic analysis of supersaturation and lysozyme crystal quality

I. Yoshizaki, T. Sato, N. Igarashi, M. Natsuisaka, N. Tanaka, H. Komatsu and S. Yoda

Synopsis: The correlation between supersaturation and hen egg-white lysozyme crystal quality was systematically studied employing atomic force microscopy and X-ray crystallography with synchrotron radiation.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1630-1633  [ doi:10.1107/S0907444901010964 ]

Crystallization and preliminary crystallographic results of apo and complex forms of human dehydroepiandrosterone sulfotransferase

M. Zhou, P. Rehse, H.-J. Chang, V. Luu-The and S.-X. Lin

Synopsis: Human dehydroepiandrosterone sulfotransferase is important for the transport of sex-hormone precursors to the peripheral tissues. It has been crystallized in the presence of substrate, of substrate and cofactor, and as an uncomplexed form. The crystals diffract to 2.15, 2.50 and 2.35  Å, respectively.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1634-1638  [ doi:10.1107/S090744490101099X ]

Crystallization of redox-insensitive Oct1 POU domain with different DNA-response elements

A. Reményi, E. Pohl, H. Schöler and M. Wilmanns

Synopsis: The DNA-binding domain of the Oct1 transcription factor has been crystallized in the presence of two DNA-response elements. Protein expression, purification, crystallization and crystal mounting were optimized in order to obtain crystals suitable for X-ray analysis.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1639-1642  [ doi:10.1107/S0907444901012148 ]

The xenograft antigen in complex with GS-1-B₄ lectin: crystallization and preliminary X-ray analysis

W. Tempel, L. A. Lipscomb, J. Rose and R. J. Woods

Synopsis: Isolectin B₄ of G. simplicifolia lectin-1 has been crystallized in the presence of the xenograft antigen.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1643-1645  [ doi:10.1107/S0907444901011064 ]

Crystallization and preliminary X-ray analysis of bacterial cytosine deaminase

G. C. Ireton, M. E. Black and B. L. Stoddard

Synopsis: Recombinant bacterial cytosine deaminase from Escherichia coli has been subcloned, overexpressed, purified and crystallized for structural analysis. The crystals belong to space group R32, with unit-cell parameters a = b = 109.1, c = 240  Å and diffract to at least 1.5  Å resolution at a synchrotron X-ray source.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1646-1649  [ doi:10.1107/S0907444901011106 ]

Overexpression, purification, crystallization and preliminary X-ray diffraction analysis of Cu,Zn superoxide dismutase from Peking duck

W. Liu, P.-W. Li, G.-P. Li, R.-H. Zhu and D.-C. Wang

Synopsis: The recombinant Peking duck Cu,Zn superoxide dismutase was overexpressed in Escherichia coli, purified to homogeneity and crystallized. In trigonal crystals of the enzyme, unusual supermolecular double-helix packing with 922 non-crystallographic symmetry has been observed in the molecular replacement analysis.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1650-1651  [ doi:10.1107/S090744490101112X ]

Overexpression, purification, crystallization and preliminary crystallographic studies on a thermostable -glycosidase from Thermus nonproteolyticus HG102

X.-Y. He, X.-Q. Wang, S.-J. Yang, W.-R. Chang and D.-C. Liang

Synopsis: The thermostable -glycosidase from T. nonproteolyticus HG102 has been cloned and expressed in E. coli. Preliminary crystallographic investigations have been carried out.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1652-1654  [ doi:10.1107/S0907444901011623 ]

Crystallization and preliminary X-ray analysis of ocr, the product of gene 0.3 of bacteriophage T7

S. S. Sturrock, D. T. F. Dryden, C. Atanasiu, J. Dornan, S. Bruce, A. Cronshaw, P. Taylor and M. D. Walkinshaw

Synopsis: Ocr, the product of gene 0.3 of bacteriophage T7, prevents the action of restriction endonucleases of the host bacteria. Ocr has been crystallized in a number of different crystal forms and X-ray data for the seleno-L-methionine-substituted form has been collected to a resolution of 1.8  Å.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1655-1658  [ doi:10.1107/S0907444901011982 ]

Crystals of ternary protein-DNA complexes composed of DNA-binding domains of c-Myb or v-Myb, C/EBP or C/EBP and tom-1A promoter fragment

T. H. Tahirov, M. Sasaki, T. Inoue-Bungo, A. Fujikawa, K. Sato, T. Kumasaka, M. Yamamoto and K. Ogata

Synopsis: c-Myb and the C/EBP family are transcriptional regulatory factors that act in concert to regulate the expression of myeloid-specific genes and v-Myb encoded by avian myeloblastosis virus is an oncogenic mutated form of c-Myb. Crystals of ternary complexes, in which various combinations of the DNA binding domains of c-Myb or v-Myb and C/EBP or C/EBP are bound to a DNA fragment from tom-1A promoter, were obtained by the vapour-diffusion method.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1659-1662  [ doi:10.1107/S0907444901011994 ]

Crystallization and preliminary X-ray analysis of the archaeosine tRNA-guanine transglycosylase from Pyrococcus horikoshii

R. Ishitani, O. Nureki, T. Kijimoto, M. Watanabe, H. Kondo, N. Nameki, N. Okada, S. Nishimura and S. Yokoyama

Synopsis: The archaeosine tRNA-guanine transglycosylase from the hyperthermophilic archaeon P. horikoshii was crystallized and data were collected to 2.2  Å. Selenomethionine-labelled protein crystals were prepared, with the aim of solving the structure using a MAD phasing method.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1663-1665  [ doi:10.1107/S0907444901012124 ]

Preliminary X-ray diffraction studies of the external functional unit RtH2-e from the Rapana thomasiana

M. Perbandt, V. Chandra, K. R. Rajashankar, K. Idakieva, K. Parvanova, W. Rypniewski, S. Stoeva, W. Voelter, N. Genov and C. Betzel

Synopsis: Rapana hemocyanin functional unit RtH2-e was isolated and crystallized. X-ray data to 3.3  Å resolution have been collected.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1666-1668  [ doi:10.1107/S0907444901012069 ]

Crystallization and preliminary X-ray analysis of a bacterial psychrophilic enzyme, phosphoglycerate kinase

D. Mandelman, M. Bentahir, G. Feller, C. Gerday and R. Haser

Synopsis: The psychrophilic enzyme phosphoglycerate kinase from the antarctic bacterium Pseudomonas sp TACII18 has been crystallized alone and in the presence of its product and inhibitor: 3-PGA and AMP-PNP. X-ray diffraction data have been collected on both types of crystals and the cocrystallized data set has been phased by molecular replacement.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1669-1670  [ doi:10.1107/S0907444901012082 ]

Purification, crystallization and preliminary X-ray diffraction studies of disintegrin (schistatin) from saw-scaled viper (Echis carinatus)

S. Tomar, S. Yadav, V. Chandra, P. Kumar and T. P. Singh

Synopsis: The heterodimeric disintegrin with a molecular weight of 14  kDa from E. carinatus venom is a potent antagonist of 4 integrins. Crystals of disintegrin belonging to space group I4₁22 with unit-cell dimensions a = b = 91.7, c = 55.7,  Å have been produced.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1671-1673  [ doi:10.1107/S0907444901012094 ]

Expression, purification, crystallization and preliminary crystallographic analysis of recombinant pteridine reductase of Trypanosoma cruzi

N. Schormann, B. Pal and D. Chattopadhyay

Synopsis: The asymmetric unit of the T. cruzi pteridine reductase crystal contains a tetramer related by non-crystallographic 222-symmetry.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1674-1676  [ doi:10.1107/S0907444901012549 ]

Crystallization and preliminary X-ray analysis of aspartate racemase from Pyrococcus horikoshii OT3

L. Liu, K. Iwata, Y. Kawarabayasi, H. Kikuchi, A. Kita, M. Yohda and K. Miki

Synopsis: Aspartate racemase from P. horikoshii has been expressed, purified and crystallized in three crystal forms. The crystals with space group P2₁ diffracted to 1.7  Å resolution using synchrotron radiation.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1677-1679  [ doi:10.1107/S0907444901012598 ]

Expression, purification, crystallization and preliminary X-ray analysis of the cathelicidin motif of the protegrin-3 precursor

J. F. Sanchez, F. Hoh, M.-P. Strub, J. M. Strub, A. Van Dorsselaer, R. Lehrer, T. Ganz, A. Chavanieu, B. Calas, C. Dumas and A. Aumelas

Synopsis: The protegrin-3 prosequence which belongs to the cathelicidin family was overexpressed in E. coli, purified to homogeneity and crystallized. Crystals were shown to diffract beyond 1.9  Å and belong to either space group P6₁22 or P6₅22 with unit-cell parameters a = b = 51.42, c = 134.25  Å and have one molecule in the asymmetric unit.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1680-1681  [ doi:10.1107/S0907444901012732 ]

Crystallization and preliminary X-ray characterization of rat liver acyl-CoA oxidase

Y. Nakajima, I. Miyahara, K. Hirotsu, Y. Nishina, K. Shiga, C. Setoyama, H. Tamaoki and R. Miura

Synopsis: The flavoenzyme acyl-CoA oxidase from rat liver has been crystallized by the hanging-drop vapour-diffusion technique using PEG 20  000 as a precipitating agent. The crystals belong to space group P2₁2₁2₁ and diffract beyond 2.5  Å resolution.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1682-1685  [ doi:10.1107/S0907444901012823 ]

Crystallization and preliminary X-ray diffraction studies of monomeric isocitrate dehydrogenase by the MAD method using Mn atoms

Y. Yasutake, S. Watanabe, M. Yao, Y. Takada, N. Fukunaga and I. Tanaka

Synopsis: Monomeric isocitrate dehydrogenase was crystallized and preliminary X-ray diffraction studies were performed by the MAD method using Mn atoms.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1686-1688  [ doi:10.1107/S0907444901012835 ]

Crystallization and preliminary X-ray studies of Trp138Phe/Val185Thr xylose isomerases from Thermotoga neapolitana and Thermoanaerobacterium thermosulfurigenes

Y. S. Kim, Y. J. Im, S. H. Rho, D. Sriprapundh, C. Vieille, S. W. Suh, J. G. Zeikus and S. H. Eom

Synopsis: Trp138Phe/Val185Thr xylose isomerases from T. neapolitana and T. thermosulfurigenes have been crystallized in a form suitable for X-ray diffaction studies. The crystals belong to the orthorhombic space groups C222₁ (TNXI mutant) and P2₁2₁2₁ (TTXI mutant).

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1689-1691  [ doi:10.1107/S0907444901012999 ]

Structural studies of the Hrp secretion system: expression, purification, crystallization and preliminary X-ray analysis of the C-terminal domain of the HrcQ_B protein from Pseudomonas syringae pv. phaseolicola

V. E. Fadouloglou, A. P. Tampakaki, N. J. Panopoulos and M. Kokkinidis

Synopsis: The C-terminal domain of the HrcQ_B protein from P. syringae was overexpressed, purified and crystallized. Native X-ray diffraction data were collected to 3.0  Å using a laboratory source.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1692-1694  [ doi:10.1107/S0907444901014548 ]

Mouse testis-brain RNA-binding protein (TB-RBP): expression, purification and crystal X-ray diffraction

J. M. Pascal, V. M. Chennathukuzhi, N. B. Hecht and J. D. Robertus

Synopsis: TB-RBP temporally and spatially controls mRNA translation; the protein has been crystallized in space group P2₁2₁2.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1695-1696  [ doi:10.1107/S0907444901013105 ]

Crystallization and preliminary X-ray analysis of RecG, a replication-fork reversal helicase from Thermotoga maritima complexed with a three-way DNA junction

M. R. Singleton, S. Scaife, N. D. Raven and D. B. Wigley

Synopsis: The replication-fork regression helicase recG from T. maritima has been crystallized in complex with a three-way DNA junction.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1697-1699  [ doi:10.1107/S0907444901013191 ]

Crystallization and preliminary X-ray diffraction analysis of cytotoxic ribonucleases from bullfrog Rana catesbeiana

J.-H. Liu, Y.-D. Liao and Y.-J. Sun

Synopsis: Three isozymes of frog ribonucleases from R. catesbeiana oocytes have been crystallized under different conditions in various space groups.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1700-1702  [ doi:10.1107/S0907444901013208 ]

Crystallization and preliminary X-ray crystallographic studies on a Fab fragment of the mouse anti-human Fas monoclonal antibody HFE7A

S. Ito, T. Takayama, H. Hanzawa, K. Ichikawa, J. Ohsumi, N. Serizawa, H. Haruyama and T. Hata

Synopsis: Crystals of an antigen-binding fragment of the mouse anti-human Fas monoclonal antibody HFE7A have been obtained. Fast optimization to produce single crystals suitable for X-ray analysis was achieved by the streak-seeding technique.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1703-1705  [ doi:10.1107/S0907444901013300 ]

Expression, characterization and crystallization of the Fv fragment of mouse antibody 3B62 from the secondary immune response

K. Murase, R. Mizutani and Y. Satow

Synopsis: The 3B62 antibody Fv expressed in the yeast P. pastoris was secreted as a heterodimer consisting of the V_L and V_H domains. It retains nitrophenol-binding affinity comparable with that of the 3B62 Fab and yields crystals of good diffraction quality.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1706-1708  [ doi:10.1107/S0907444901013427 ]

Crystallization and preliminary X-ray diffraction analysis of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua)

I. Leiros, O. Lanes, O. Sundheim, R. Helland, A. O. Smalås and N. P. Willassen

Synopsis: Uracil-DNA glycosylase (UDG) is a DNA-repair enzyme involved in the removal of uracil from DNA. Atlantic cod UDG has been crystallized by the vapour-diffusion method using sodium citrate as the precipitant at pH 7.5.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1709-1711  [ doi:10.1107/S0907444901013361 ]

Tobacco uroporphyrinogen-III decarboxylase: characterization, crystallization and preliminary X-ray analysis

B. M. Martins, B. Grimm, H.-P. Mock, G. Richter, R. Huber and A. Messerschmidt

Synopsis: Uroporphyrinogen-III decarboxylase from the tobacco plant is a plastidial enzyme involved in the biosynthesis of chlorophyll and haem. Sedimentation equilibrium and dynamic light scattering revealed a dimeric form in solution. Good quality diffraction crystals were obtained using synchrotron radiation.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1712-1714  [ doi:10.1107/S0907444901013452 ]

Crystallization and preliminary X-ray analysis of a Trx domain of human thioredoxin-like protein

X. Chen, J. Jin, Y. Gao, Q. Guo, Y. Sun, H. Tang, J. Yuan, B. Qiang and Z. Rao

Synopsis: Crystals of the catalytic domain of a human thioredoxin-like protein have been obtained by the vapour-diffusion method. The results of the preliminary X-ray diffraction analysis of these crystals are discussed.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1715-1717  [ doi:10.1107/S0907444901013798 ]

Crystallization and X-ray diffraction measurements on recombinant molbindin, MopII, from Clostridium pasteurianum

J. A. Harrison, A. W. Schüttelkopf, D. H. Boxer and W. N. Hunter

Synopsis: A molybdate/tungstate binding protein has been crystallized in the presence and absence of oxyanion. High-resolution diffraction data have been recorded.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1718-1721  [ doi:10.1107/S0907444901014020 ]

Preparation and crystallization of a Bacillus subtilis arsenate reductase

Z. Guan, L. Hederstedt, J. Li and X.-D. Su

Synopsis: An arsenate reductase from B. subtilis has been expressed, purified and crystallized. Data in space group P2₁2₁2₁ have been collected to high resolution from crystals of both wild-type and SeMet proteins.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1722-1725  [ doi:10.1107/S090744490101410X ]

Expression, purification and crystallization of Aspergillus nidulans NmrA, a negative regulatory protein involved in nitrogen-metabolite repression

C. E. Nichols, S. Cocklin, A. Dodds, J. Ren, H. Lamb, A. R. Hawkins and D. K. Stammers

Synopsis: The negative transcriptional regulator NmrA which is responsible for nitrogen-metabolite repression in certain fungi has been expressed and purified. Three crystal forms have been obtained, two of which appear suitable for high-resolution structure determination.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1726-1728  [ doi:10.1107/S0907444901014214 ]

Crystallization and preliminary X-ray diffraction analysis of glutathione-dependent dehydroascorbate reductase from spinach chloroplasts

E. Mizohata, M. Kumei, H. Matsumura, T. Shimaoka, C. Miyake, T. Inoue, A. Yokota and Y. Kai

Synopsis: Glutathione-dependent dehydroascorbate reductase from spinach chloroplasts has been crystallized. The crystals diffract to 2.2  Å and belong to space group C2.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1729-1731  [ doi:10.1107/S0907444901014202 ]

Crystallization and preliminary X-ray crystallographic analysis of native and selenomethionyl recombinant tabtoxin-resistance protein complexed with acetyl-coenzyme A

H. He, Y. Ding, Z. Cao, Y. Shao, M. Bartlam, H. Tang, F. Jiang, Y. Liu, J. Liu, N. Zhao and Z. Rao

Synopsis: recombinant tabtoxin-resistance protein complexed with acetyl-coA as well as its selenomethionine derivative were crystallized. The crystals from the derivative were found to belong to two crystal forms and diffracted to high resolution.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1732-1734  [ doi:10.1107/S0907444901013002 ]

Crystallization of quinohaemoprotein alcohol dehydrogenase from Comamonas testosteroni: crystals with unique optical properties

A. Oubrie, E. G. Huizinga, H. J. Rozeboom, K. H. Kalk, G. A. H. de Jong, J. A. Duine and B. W. Dijkstra

Synopsis: Crystals of the quinohaemoprotein alcohol dehydrogenase from C. testosteroni were obtained and a 2.44  Å resolution data set has been collected. An approximate orientation of the haem groups has been derived by inspection of the crystals using plane-polarized light.

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1735-1738  [ doi:10.1107/S0907444901013221 ]

The 1.6  Å resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge

M. Milani, L. Andolfi, S. Cannistraro, M. P. Verbeet and M. Bolognesi

Synopsis: A surface disulfide has been engineered in popular plastocyanin, at residues 21-25. The site is at about 20  Å from plastocyanin type I copper, whose coordination sphere is not affected by the engineered disulfide. The mutant plastocyanin has been successfully employed for chemisorption on gold substrates.

PDB reference: 1jxg

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1739-1742  [ doi:10.1107/S0907444901013993 ]

Atomic resolution structure of endoglucanase Cel5A in complex with methyl 4,4^II,4^III,4^IV-tetrathio--cellopentoside highlights the alternative binding modes targeted by substrate mimics

A. Varrot, M. Schülein, S. Fruchard, H. Driguez and G. J. Davies

Synopsis: The 1.1  Å structure of a retaining endocellulase, Cel5A, in complex with methyl 4,4^II,4^III,4^IV-tetrathio--cellopentoside reveals the binding of five pyranosides, all in the ⁴C₁ chair conformation, occupying the -3, -2, +1 and +2 subsites.

PDB reference: 1h5v

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1743-1746  [ doi:10.1107/S0907444901013531 ]

Crystallographic evidence for doxorubicin binding to the receptor-binding site in Clostridium botulinum neurotoxin B

S. Eswaramoorthy, D. Kumaran and S. Swaminathan

Synopsis: The binding site of doxorubicin is identified from crystal structure analysis.

PDB reference: 1i1e

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1747-1751  [ doi:10.1107/S0907444901014019 ]

Streptococcus pneumonia YlxR at 1.35  Å shows a putative new fold

J. Osipiuk, P. Górnicki, L. Maj, I. Dementieva, R. Laskowski and A. Joachimiak

Synopsis: Structure of YlxR homologue from S. pneumonia was determined using MAD phasing and synchrotron radiation. The protein shows a new fold.

PDB reference: 1g2r

Online 25 October 2001

 

Acta Cryst. (2001). D57, 1752-1753  [ doi:10.1107/S0907444901013385 ]

Automated data processing on beamline FIP (BM30A) at ESRF

J.-L. Ferrer

Synopsis: ADP is a fully automated data-processing program for protein crystallography. It performs analysis and makes choices the way crystallographers usually do on a beamline when using standard integration software.

Online 25 October 2001

    

Acta Cryst. (2001). D57, 1754  [ doi:10.1107/S0907444901017115 ]

The high-mosaicity illusion: revealing the true physical characteristics of macromolecular crystals. Erratum

H. D. Bellamy, E. H. Snell, J. Lovelace, M. Pokross and G. E. O. Borgstahl

Synopsis: Erratum to Acta Cryst. (2000), D56, 986-995.

Online 25 October 2001