Acta Crystallographica Section D

Biological Crystallography

Volume 58, Part 8 (August 2002)



HTML versionpdf versionsupplementary materialssimilar papers buy article online

Acta Cryst. (2002). D58, 1346-1349    [doi:10.1107/S0907444902009137]

A stepwise optimization of crystals of rhamnogalacturonan lyase from Aspergillus aculeatus

R. Kadirvelraj, P. Harris, J.-C. N. Poulsen, S. Kauppinen and S. Larsen

Abstract: Recombinant rhamnogalacturonan lyase from Aspergillus aculeatus has been crystallized by a stepwise procedure and X-ray diffraction data have been collected. The crystals were grown using hanging-drop vapour-diffusion and microseeding techniques. Crystals were obtained showing a flat plate morphology. The crystallization conditions were 20% PEG 4000, 9% PEG 400, 0.1  M (NH4)2SO4 and 0.1  M sodium acetate pH 4.4. These crystals diffracted to a resolution of 1.5  Å. The unit-cell parameters are a = b = 77.0, c = 170.8  Å with the possible space group P43212 or P41212. There is most likely to be one molecule in the asymmetric unit, leading to a calculated solvent content of approximately 47% for the crystals.

Online 20 July 2002


pdfdisplay filedownload file

PDF file (16.3 kbytes)
Supplementary material


Notes:

To open or display or play some files, you may need to set your browser up to use the appropriate software. See the full list of file types for an explanation of the different file types and their related mime types and, where available links to sites from where the appropriate software may be obtained.

The download button will force most browsers to prompt for a file name to store the data on your hard disk.

Where possible, images are represented by thumbnails.


Copyright © International Union of Crystallography
IUCr Webmaster