Acta Crystallographica Section D

Biological Crystallography

Volume 58, Part 12 (December 2002)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: View of several protein crystals in their plates or isolated. The proteins are produced from unknown ORFs of E. coli, within a structural genomics programme. The crystals were obtained with a nanodrop dispensing robot. The initial volume of the drops was 100 + 100 nl. Most crystals appeared between 4 and 48 h (p. 2109).

editorial


 

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Acta Cryst. (2002). D58, 1971  [ doi:10.1107/S0907444902021273 ]

Editorial

J. P. Glusker

Online 26 November 2002


research papers


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Acta Cryst. (2002). D58, 1972-1982  [ doi:10.1107/S0907444902016566 ]

The structures of Micrococcus lysodeikticus catalase, its ferryl intermediate (compound II) and NADPH complex

G. N. Murshudov, A. I. Grebenko, J. A. Brannigan, A. A. Antson, V. V. Barynin, G. G. Dodson, Z. Dauter, K. S. Wilson and W. R. Melik-Adamyan

Synopsis: The crystal structure of the bacterial catalase from M. lysodeikticus has been refined at 0.88 Å using the gene-derived sequence, revealing the active site with unprecedented accuracy. In addition, the structures of the ferryl intermediate of the catalase (compound II) at 1.96 Å resolution and the catalase complex with NADPH at 1.83 Å resolution have been determined.

PDB references: 1gwe, 1hbz, 1gwf and 1gwh

Online 26 November 2002


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Acta Cryst. (2002). D58, 1983-1991  [ doi:10.1107/S090744490201394X ]

The impact of Glu[rightwards arrow]Ala and Glu[rightwards arrow]Asp mutations on the crystallization properties of RhoGDI: the structure of RhoGDI at 1.3 Å resolution

A. Mateja, Y. Devedjiev, D. Krowarsch, K. Longenecker, Z. Dauter, J. Otlewski and Z. S. Derewenda

Synopsis: Studies of a number of Glu[rightwards arrow]Ala single and multiple mutants of RhoGDI show that such mutations may be generally useful to enhance protein crystallizability. One of the mutants yielded data to 1.2 Å resolution, showing that surface mutants can be used to dramatically improve the resolution of X-ray data.

PDB reference: 1kmt

Online 26 November 2002


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Acta Cryst. (2002). D58, 1992-2000  [ doi:10.1107/S0907444902015433 ]

Structures of Plasmodium falciparum triosephosphate isomerase complexed to substrate analogues: observation of the catalytic loop in the open conformation in the ligand-bound state

S. Parthasarathy, H. Balaram, P. Balaram and M. R. N. Murthy

Synopsis: Structures of triosephosphate isomerase (TIM) from the malarial parasite P. falciparum (Pf) complexed to the substrate analogues 3-phosphoglycerate and glycerol-3-phosphate have been determined at 2.4 Å resolution. The catalytic loop of TIM adopts a novel `loop-open' conformation in these complexes. These structures provide insight into the design of inhibitors specific for the malarial enzyme.

PDB references: 1m7o and 1m7p

Online 26 November 2002


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Acta Cryst. (2002). D58, 2001-2008  [ doi:10.1107/S0907444902014695 ]

Structures of Ser205 mutant plasmepsin II from Plasmodium falciparum at 1.8 Å in complex with the inhibitors rs367 and rs370

O. A. Asojo, E. Afonina, S. V. Gulnik, B. Yu, J. W. Erickson, R. Randad, D. Medjahed and A. M. Silva

Synopsis: Two 1.8 Å resolution crystal structures of the Ser205 mutant plasmepsin II from P. falciparum in complex with two Phe-Leu core inhibitors that incorporate tetrahedral transition-state mimetic hydroxypropylamine are described.

PDB references: 1lee and 1lf2

Online 26 November 2002


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Acta Cryst. (2002). D58, 2009-2017  [ doi:10.1107/S0907444902014683 ]

Refinement of the arginine kinase transition-state analogue complex at 1.2 Å resolution: mechanistic insights

M. S. Yousef, F. Fabiola, J. L. Gattis, T. Somasundaram and M. S. Chapman

Synopsis: The transition-state complex of this 42 kDa homologue of creatine kinase has been refined at 1.2 Å resolution. It indicates that precise positioning of substrates and restriction of the active-site motion are important components in catalysis.

PDB reference: 1m15

Online 26 November 2002


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Acta Cryst. (2002). D58, 2018-2023  [ doi:10.1107/S0907444902015548 ]

Structure of a circularly permuted phosphoglycerate kinase

P. Tougard, T. Bizebard, M. Ritco-Vonsovici, P. Minard and M. Desmadril

Synopsis: The crystallographic structure of a circularly permuted form of yeast PGK has been determined to a resolution of 2.3 Å. The overall fold of the protein is very similar to that of the wild-type protein, directly demonstrating that the continuity of a folding unit is not relevant to the folding process of the whole protein.

PDB reference: 1fw8

Online 26 November 2002


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Acta Cryst. (2002). D58, 2024-2030  [ doi:10.1107/S0907444902015524 ]

An investigation of magnetic field effects on the dissolution of lysozyme crystal and related phenomena

D. C. Yin, Y. Inatomi, N. I. Wakayama, W. D. Huang and K. Kuribayashi

Synopsis: The diffusivity of lysozyme molecules was deduced to decrease under a magnetic field, based on the discovery of magnetic damping of the dissolution process using a Mach-Zehnder interferometer. In the same experiment, a redistribution of the concentration before crystal growth was also found.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2031-2037  [ doi:10.1107/S0907444902015809 ]

Structure of human carbonmonoxyhemoglobin at 2.16 Å: a snapshot of the allosteric transition

M. K. Safo, J. C. Burnett, F. N. Musayev, S. Nokuri and D. J. Abraham

Synopsis: A new structure of carbonmonoxyhemoglobin solved at 2.16 Å is reported. The new structure, obtained using a lower pH, shows significant strain at the [alpha]1[beta]2 ([alpha]2[beta]1) interface, suggests initiation of the R to T allosteric transition.

PDB reference: 1ljw

Online 26 November 2002


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Acta Cryst. (2002). D58, 2038-2042  [ doi:10.1107/S0907444902016426 ]

Structure of mutant human carbonmonoxyhemoglobin C ([beta]E6K) at 2.0 Å resolution

J. C. Dewan, A. Feeling-Taylor, Y. A. Puius, L. Patskovska, Y. Patskovsky, R. L. Nagel, S. C. Almo and R. E. Hirsch

Synopsis: The first high-resolution crystal structure for liganded hemoglobin C, a mutant hemoglobin that forms crystals in red blood cells of individuals with CC and SC diseases, was obtained from a COHbC crystal grown in concentrated potassium phosphate buffer. The dynamic structure of the [beta]EK6 side chain could potentially support a number of specific polar interactions that may contribute to enhanced nucleation kinetics for HbC relative to HbA.

PDB reference: 1k1k

Online 26 November 2002


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Acta Cryst. (2002). D58, 2043-2054  [ doi:10.1107/S0907444902016724 ]

Automatic modeling of protein backbones in electron-density maps via prediction of C[alpha] coordinates

T. R. Ioerger and J. C. Sacchettini

Synopsis: A new computational procedure called CAPRA is described that predicts coordinates of C[alpha] atoms in density maps and outputs chains of C[alpha] atoms representing the backbone of the protein.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2055-2059  [ doi:10.1107/S0907444902017420 ]

Structure determination of a cocaine hydrolytic antibody from a pseudomerohedrally twinned crystal

N. A. Larsen, A. Heine, P. de Prada, E.-R. Redwan, T. O. Yeates, D. W. Landry and I. A. Wilson

Synopsis: The structure of a cocaine hydrolytic antibody has been determined from a pseudomerohedrally twinned crystal. The lattice is primitive monoclinic with higher apparent primitive orthorhombic symmetry.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2060-2065  [ doi:10.1107/S0907444902017183 ]

Crystallization in the presence of glycerol displaces water molecules in the structure of thaumatin

C. Charron, A. Kadri, M.-C. Robert, R. Giegé and B. Lorber

Synopsis: Glycerol displaces water molecules bound to thaumatin.

PDB references: 1lxz and 1ly0

Online 26 November 2002


 

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Acta Cryst. (2002). D58, 2066-2075  [ doi:10.1107/S0907444902016992 ]

Multiple rotation function

A. Urzhumtsev and L. Urzhumtseva

Synopsis: A simultaneous analysis of several rotation functions allows the model orientation to be found in situations when the standard search fails. An automated procedure has been developed to apply such an analysis routinely.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2076-2081  [ doi:10.1107/S0907444902016736 ]

The influence of detergents on the solubility of membrane proteins

M. A. Rosenow, C. L. Magee, J. C. Williams and J. P. Allen

Synopsis: The dependence of protein solubility on ionic strength was measured for the bacterial reaction center in different detergents and amphiphiles. The detergent and amphiphile combinations that yielded the highest solubility of the protein are correlated with those that yield protein crystals.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2082-2086  [ doi:10.1107/S0907444902016360 ]

Statistical density modification with non-crystallographic symmetry

T. C. Terwilliger

Synopsis: Statistical density modification can make use of NCS in a crystal and can include estimates of the deviations from perfect NCS.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2087-2095  [ doi:10.1107/S0907444902017511 ]

Convergent-beam method in macromolecular crystallography

J. X. Ho, J. R. Ruble, T. R. McInnis, D. C. Carter, H. Huang and W. M. Gibson

Synopsis: The convergent-beam method of data collection for macromolecular crystals is described.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2096-2101  [ doi:10.1107/S0907444902018838 ]

S-SAD, Se-SAD and S/Se-SIRAS using Cu K[alpha] radiation: why wait for synchrotron time?

C. T. Lemke, G. D. Smith and P. L. Howell

Synopsis: The structure of E. coli argininosuccinate synthetase (EAS) has been determined using S-SAD, Se-SAD and S/Se-SIRAS data measured with Cu K[alpha] radiation.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2102-2108  [ doi:10.1107/S0907444902016359 ]

Parallel cloning, expression, purification and crystallization of human proteins for structural genomics

H. Ding, H. Ren, Q. Chen, G. Fang, L. Li, R. Li, Z. Wang, X. Jia, Y. Liang, M. Hu, Y. Li, J. Luo, X. Gu, X.-D. Su, M. Luo and S. Lu

Synopsis: Insights are gained into high-throughput target selection, cloning, expression and crystallization of human genomic proteins. For example, there was a marked difference seen bewteen the ease of expression of proteins of molecular weight (MW) < 25 kDa (93% success), compared with proteins > 25 kDa MW, which were less easy to express (61% success).

Online 26 November 2002


structural genomics papers


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Acta Cryst. (2002). D58, 2109-2115  [ doi:10.1107/S0907444902013938 ]

A medium-throughput crystallization approach

G. Sulzenbacher, A. Gruez, V. Roig-Zamboni, S. Spinelli, C. Valencia, F. Pagot, R. Vincentelli, C. Bignon, A. Salomoni, S. Grisel, D. Maurin, C. Huyghe, K. Johansson, A. Grassick, A. Roussel, Y. Bourne, S. Perrier, L. Miallau, P. Cantau, E. Blanc, M. Genevois, A. Grossi, A. Zenatti, V. Campanacci and C. Cambillau

Synopsis: In the context of a medium-scale structural genomics program, a crystallization approach was based on a two-step procedure: a large screening step employing robotics, followed by manual or automated optimization of the crystallization conditions. A nanodrop dispensing robot (50-200 nl) proved to be useful for obtaining diffracting crystals while using small amounts of protein. In 10 months, 25 proteins were subjectred to crystallization, 13 yielded crystals among which ten led to usuable data sets and five to structures.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2116-2121  [ doi:10.1107/S0907444902017055 ]

Autotracing of Escherichia coli acetate CoA-transferase [alpha]-subunit structure using 3.4 Å MAD and 1.9 Å native data

S. Korolev, O. Koroleva, K. Petterson, M. Gu, F. Collart, I. Dementieva and A. Joachimiak

Synopsis: This study demonstrates that phases obtained at low resolution (>3.0 Å) from crystals of SeMet-labeled protein can be successfully used for automated structure determination.

PDB reference: 1k6d

Online 26 November 2002


crystallization papers


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Acta Cryst. (2002). D58, 2122-2124  [ doi:10.1107/S0907444902011204 ]

Purification, crystallization and preliminary X-ray analysis of the disintegrin contortrostatin from Agkistrodon contortrix contortrix snake venom

N. Moiseeva, S. D. Swenson, F. S. Markland Jr and R. Bau

Synopsis: Contortrostatin, a small homodimeric protein (64 residues per chain) from southern copperhead snake venom, has been crystallized successfully. Crystals of the title compound, which is a member of the disintegrin family of proteins, diffract up to a maximum resolution of 1.7 Å.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2125-2126  [ doi:10.1107/S0907444902015469 ]

Crystallization of an [alpha]-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii

N. Li, B. K. C. Patel, B. N. Mijts and K. Swaminathan

Synopsis: The first crystallographic study of an [alpha]-amylase from an organism that is both thermophilic and halophilic, at 1.89 Å resolution.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2127-2130  [ doi:10.1107/S0907444902015020 ]

Crystallographic studies of a novel DNA-binding domain from the yeast transcriptional activator Ndt80

S. P. Montano, M. Pierce, M. L. Coté, A. K. Vershon and M. M. Georgiadis

Synopsis: The Ndt80 protein is a transcriptional activator that plays a key role in the progression of the meiotic divisions in the yeast Saccharomyces cerevisiae. Crystals of the DNA-binding domain of Ndt80 in two distinct lattices have been obtained, for which diffraction data extend to 2.3 Å resolution.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2131-2134  [ doi:10.1107/S0907444902015482 ]

The production, purification and crystallization of a soluble heterodimeric form of a highly selected T-cell receptor in its unliganded and liganded state

C. S. Clements, L. Kjer-Nielsen, W. A. MacDonald, A. G. Brooks, A. W. Purcell, J. McCluskey and J. Rossjohn

Synopsis: This report details the production, purification, crystallization and preliminary diffraction analysis of a T-cell receptor.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2135-2137  [ doi:10.1107/S0907444902015494 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding domain of flocculin, a cell-adhesion molecule from Saccharomyces carlsbergensis

M. Groes, K. Teilum, K. Olesen, F. M. Poulsen and A. Henriksen

Synopsis: The recombinant carbohydrate-binding domain of the cell-surface lectin flocculin from brewer's yeast has been identified, purified and crystallized.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2138-2140  [ doi:10.1107/S0907444902015512 ]

Crystallization and preliminary X-ray analysis of the ytxM gene product from Bacillus subtilis

M. M. Mehanni, A. P. Turnbull, S. E. Sedelnikova, P. J. Baker, S. Foster and D. W. Rice

Synopsis: The B. subtilis ytxM gene product has been crystallized and a MAD data set has been collected to 2.0 Å resolution.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2141-2144  [ doi:10.1107/S0907444902015536 ]

Crystallization and preliminary X-ray analysis of the tumor metastasis factor p37

R. Reutzel, S. K. Boehlein, L. Govindasamy, R. B. Brenes, M. Agbandje-McKenna, S. M. Schuster and R. McKenna

Synopsis: P37, an outer-membrane bacterial protein from Mycoplasma hyorhinis whose presence on the surface of many tumor cells correlates highly with increased neoplastic invasivity and metastasis, has been overexpressed in Escherichia coli, purified by affinity chromatography and crystallized.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2145-2146  [ doi:10.1107/S0907444902015573 ]

Crystallization and preliminary X-ray crystallographic analysis of acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa

S. J. Eom, H. J. Ahn, H.-J. Yoon, B. I. Lee, S. H. Bae, S. H. Baek and S. W. Suh

Synopsis: Acetohydroxy acid isomeroreductase from P. aeruginosa was crystallized. X-ray data have been collected to 2.0 Å resolution using synchrotron radiation (P213, a = b = c = 184.38 Å).

Online 26 November 2002


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Acta Cryst. (2002). D58, 2147-2149  [ doi:10.1107/S090744490201572X ]

Crystallization and preliminary X-ray analysis of the trehalose/maltose ABC transporter MalFGK2 from Thermococcus litoralis

A. Schiefner, K. Diederichs, K. Hashimoto, W. Boos and W. Welte

Synopsis: The crystallization and preliminary characterization of the MalFGK2 transport complex from T. litoralis is presented.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2150-2152  [ doi:10.1107/S0907444902015743 ]

Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of Pseudomonas aeruginosa L-arginine deiminase

Y. Oudjama, C. Tricot, V. Stalon and J. Wouters

Synopsis: Overexpression, purification and crystallization of P. aeruginosa L-arginine deiminase allowed collection of a complete MAD data set to 3.2 Å resolution.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2153-2156  [ doi:10.1107/S090744490201569X ]

Co-crystallization of Staphylococcus aureus peptide deformylase (PDF) with potent inhibitors

M. S. Harris, J. H. Bock, G. Choi, J. S. Cialdella, K. A. Curry, M. R. Deibel Jr, E. J. Jacobsen, V. P. Marshall, R. W. Murray Jr, A. F. Vosters, C. L. Wolfe, A. W. Yem and E. T. Baldwin

Synopsis: Diffraction-quality Se-met crystals of S. aureus PDF were prepared that belong to space group C2221 with unit-ell parameters of a = 94.1 b = 121.9 c = 47.6 Å. Crystals were also prepared with three PDF inhibitors: thiorphan, actinonin and PNU-172550.

Online 26 November 2002


 

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Acta Cryst. (2002). D58, 2157-2158  [ doi:10.1107/S0907444902015810 ]

Crystallization and preliminary X-ray diffraction studies of NusG, a protein shared by the transcription and translation machines

M. Andrykovitch, W. Guo, K. M. Routzahn, Y. Gu, D. E. Anderson, L. S. Reshetnikova, J. R. Knowlton, D. S. Waugh and X. Ji

Synopsis: N-utilization factor G (NusG) from A. aeolicus has been overexpressed, purified and crystallized. The crystals diffract to 2.45 Å resolution.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2159-2161  [ doi:10.1107/S0907444902015858 ]

Cloning, expression, purification and preliminary X-ray crystallographic studies of 2-methylisocitrate lyase from Salmonella typhimurium

D. K. Simanshu, P. S. Satheshkumar, S. Parthasarathy, H. S. Savithri and M. R. N. Murthy

Synopsis: 2-Methylisocitrate lyase from S. typhimurium IFO 12529 has been cloned, overexpressed and crystallized. The crystal belongs to space group P212121, with unit-cell parameters a = 63.600, b = 100.670, c = 204.745 Å. A complete data set to 2.5 Å resolution has been collected.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2162-2164  [ doi:10.1107/S0907444902016050 ]

Crystallization and preliminary X-ray structure analysis of isocitrate dehydrogenase from two hyperthermophiles, Aeropyrum pernix and Thermotoga maritima

M. Karlström, I. H. Steen, G. Tibbelin, T. Lien, N.-K. Birkeland and R. Ladenstein

Synopsis: Extremely thermostable isocitrate dehydrogenases from A. pernix and T. maritima were crystallized and X-ray diffraction data were collected to 2.6 and 2.8 Å, respectively. The crystallization and preliminary crystallographic analysis are described for both enzymes.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2165-2167  [ doi:10.1107/S0907444902016062 ]

Purification, characterization and preliminary crystallographic studies of a PR-10 protein from Pachyrrhizus erosus seeds

F. Wu, Y. Li, S. Chang, Z. Zhou, F. Wang, X. Song, Y. Lin and W. Gong

Synopsis: A new PR-10 family protein SPE16 with ribonuclease activity was purified from the seeds of P. erosus as a constitutive protein and has been crystallized.

Online 26 November 2002


 

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Acta Cryst. (2002). D58, 2168-2169  [ doi:10.1107/S0907444902016396 ]

Crystallization and preliminary X-ray diffraction analysis of FKBP52 N-terminal domain

P. Li, C. Shu, B. Wu, Y. Ding, B. Shen and Z. Rao

Synopsis: The N-terminal (amino acids 1-140) domain of human FKBP52 has been crystallized. The crystals belong to space group P21 with unit-cell parameters a = 27.8, b = 58.4, c = 70.9 Å, [beta] = 98.3° and diffract to 2.4 Å resolution limit.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2170-2172  [ doi:10.1107/S090744490201644X ]

Expression, crystallization and preliminary X-ray studies of the recombinant PTB domain of human dok-5 protein

N. Shi, W. Zhou, K. Tang, Y. Gao, J. Jin, F. Gao, X. Peng, M. Bartlam, B. Qiang, J. Yuan and Z. Rao

Synopsis: The PTB domain of human dok-5 protein has been crystallized as well as a selenomethionmine-substituted protein for MAD phasing determination. The crystals belong to space group P3221 (a = b = 75.9, c = 108.0 Å) and diffract up to 2.3 Å resolution using synchrotron radiation.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2173-2174  [ doi:10.1107/S0907444902016803 ]

Crystallization and preliminary X-ray diffraction analysis of naphthalene dioxygenase from Rhodococcus sp. strain NCIMB 12038

Z. A. Malik, C. C. R. Allen, L. Gakhar, D. A. Lipscomb, M. J. Larkin and S. Ramaswamy

Synopsis: Naphthalene dioxygenase from Rhodococcus has been crystallized in space group P212121. The crystals diffract to 2.3 Å resolution.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2175-2176  [ doi:10.1107/S0907444902016815 ]

Crystallization and preliminary X-ray diffraction analysis of a thermostable D-hydantoinase from the mesophilic Bacillus sp. AR9

V. Agrawal, R. Sharma, R. M. Vohra and K. V. R. Kishan

Synopsis: A thermostable D-hydantoinase from mesophilic Bacillus sp. AR9 was crystallized and diffracted to 2.3 Å resolution having unit-cell parameters a = b = 129.55, c = 102.8 Å, [alpha] = [beta] = 90, [gamma] = 120°.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2177-2179  [ doi:10.1107/S0907444902016827 ]

Crystallization of Pichia pastoris lysyl oxidase

M. Lee, K. Willingham, D. Langley, M. J. Maher, A. E. Cohen, P. J. Ellis, J. A. Kuchar, D. M. Dooley, H. C. Freeman and J. M. Guss

Synopsis: The lysyl oxidase isolated from the yeast P. pastoris has been crystallized and synchrotron data have been recorded to a resolution of 1.65 Å.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2180-2181  [ doi:10.1107/S0907444902017067 ]

Preliminary crystallographic studies of the creatinine amidohydrolase from Pseudomonas putida

K. Ito, N. Kanada, T. Inoue, K. Furukawa, K. Yamashita, N. Tanaka, K. T. Nakamura, Y. Nishiya, A. Sogabe and T. Yoshimoto

Synopsis: Creatinine amidohydrolase (creatininase; EC 3.5.2.10) from P. putida has been overexpressed in E. coli and crystallized by the hanging-drop method.

Online 26 November 2002


 

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Acta Cryst. (2002). D58, 2182-2183  [ doi:10.1107/S0907444902017432 ]

Crystallization and preliminary X-ray crystallographic studies on the class II cholesterol oxidase from Burkholderia cepacia containing bound flavin

R. Aunpad, S. P. Muench, P. J. Baker, S. Sedelnikova, W. Panbangred, N. Doukyu, R. Aono and D. W. Rice

Synopsis: Crystals of B. cepacia cholesterol oxidase, a 3[beta]-hydroxysteroid oxidase containing covalently bound FAD, were obtained by the hanging-drop method using polyethylene glycol as a precipitant. The crystals diffract to beyond 2.0 Å and belong to space group P3121, with approximate unit-cell parameters a = b = 119.6, c = 101.1 Å and one subunit in the asymmetric unit.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2184-2186  [ doi:10.1107/S0907444902017869 ]

Crystallization and preliminary X-ray analysis of the catalase-peroxidase KatG from Burkholderia pseudomallei

X. Carpena, J. Switala, S. Loprasert, S. Mongkolsuk, I. Fita and P. C. Loewen

Synopsis: Crystals of the catalase-peroxidase from B. pseudomallei diffract to beyond 1.8 Å.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2187-2190  [ doi:10.1107/S0907444902017535 ]

Expression, purification, crystallization and preliminary X-ray diffraction studies of human cathepsin F complexed with an irreversible vinyl sulfone inhibitor

J. D. Ho, Y. Meltser, J. J. Buggy, J. T. Palmer, K. C. Elrod, H. Chan, K. D. Mortara and J. R. Somoza

Synopsis: A human papain-family cysteine protease has been expressed in P. pastoris, purified by conventional chromatography and crystallized.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2191-2193  [ doi:10.1107/S0907444902017894 ]

Expression, purification, crystallization and preliminary characterization of an HHED aldolase homologue from Escherichia coli K12

A. Wright, A. Blewett, V. Fulop, R. Cooper, S. Burrows, C. Jones and D. Roper

Synopsis: An ORF from the E. coli K12 genome with 56% sequence identity to 2,4-dihydroxy-hept-2-ene-1,7-dioic acid (HHED) aldolase has been cloned, expressed and purified. Crystals obtained from the purified recombinant enzyme, belonging to space group C2221, diffracted to a resolution of 1.78 Å.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2194-2197  [ doi:10.1107/S0907444902015445 ]

Co-crystallization of the human nuclear cap-binding complex with a m7GpppG cap analogue using protein engineering

C. Mazza, A. Segref, I. W. Mattaj and S. Cusack

Synopsis: A long solvent-exposed coiled coil in the large subunit (CBP80) of the heterodimeric nuclear cap-binding complex (CBC) has been truncated to permit crystallization of CBC with the cap analogue m7GpppG in two crystal forms diffracting to 2.15 and 2.3 Å resolution.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2198-2200  [ doi:10.1107/S0907444902015457 ]

The 2.2 Å resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate

J.-F. Gaucher, M. Selkti, T. Prangé and A. Tomas

Synopsis: The structure of the thermolysin (TLN) crystallized in the presence of SCN- suggests explanations for the solubility increase of TLN and for the inhibitory effect of high SCN- concentrations on the TLN activity.

PDB reference: 1gxw

Online 26 November 2002


short communications


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Acta Cryst. (2002). D58, 2201-2204  [ doi:10.1107/S0907444902017006 ]

Structure of the Humicola insolens cellobiohydrolase Cel6A D416A mutant in complex with a non-hydrolysable substrate analogue, methyl cellobiosyl-4-thio-[beta]-cellobioside, at 1.9 Å

A. Varrot, T. P. Frandsen, H. Driguez and G. J. Davies

Synopsis: The complex of the H. insolens cellobiohydrolase Cel6A in complex with a thiooligosaccharide substrate analogue has been determined at 1.9 Å resolution.

PDB reference: 1gz1

Online 26 November 2002


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Acta Cryst. (2002). D58, 2205-2208  [ doi:10.1107/S0907444902015500 ]

When pseudosymmetry and merohedral twinning come across: the case of the d(ApTpApTpApT) oligonucleotide in a hexagonal lattice

N. G. A. Abrescia and J. A. Subirana

Synopsis: Crystallization analyasis of twinned diffraction data of d(ApTpApTpApT) in a hexagonal packing.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2209-2212  [ doi:10.1107/S0907444902016402 ]

Structure of Escherichia coli pyruvate formate-lyase with pyruvate

L. Lehtiö, V.-M. Leppänen, J. W. Kozarich and A. Goldman

Synopsis: The structure of inactive pyruvate formate-lyase in complex with a natural substrate, pyruvate, has been solved at 2.7 Å resolution.

PDB reference: 1mzo

Online 26 November 2002


 

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Acta Cryst. (2002). D58, 2213-2215  [ doi:10.1107/S0907444902016384 ]

Rapid automatic NCS identification using heavy-atom substructures

T. C. Terwilliger

Synopsis: A rapid algorithm for identifying NCS in heavy-atom sites is described.

Online 26 November 2002


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Acta Cryst. (2002). D58, 2216  [ doi:10.1107/S0907444902019790 ]

FOX two-dimensional X-ray focusing optic

Online 26 November 2002


 

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Acta Cryst. (2002). D58, 2216  [ doi:10.1107/S0907444902021261 ]

New developments in protein-crystallization platforms and barcoding for cryogenic storage

Online 26 November 2002


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