 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 6]](za0079fig6mag.jpg)
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Figure 6
Conceptual depiction of how detergent:protein ratios vary with protein concentration during an ultrafiltration experiment. At the start of the experiment, there is a low protein concentration and excess detergent is present as protein-free micelles. If the molecular-weight cutoff of the protein concentrator is large, the micelles spin through the filter of the concentrator and do not concentrate during the experiment. Although the absolute concentration of micelles and monomers does not change, their molar ratio relative to protein–detergent complexes decreases. Therefore, the final ratio of protein-free detergent micelles and monomers to protein–detergent complexes depends on both the starting concentration of the detergent and the final concentration of protein. Initial detergent concentrations close to the critical micellar concentration of the detergent are likely to be ideal. |
![[Figure 6]](za0079fig6.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
