Biological Crystallography

 

Acta Cryst. (2003). D59, 405-415  [ doi:10.1107/S090744490202111X ]

Structural analysis of Tityus serrulatus Ts1 neurotoxin at atomic resolution: insights into interactions with Na⁺ channels

C. B. Pinheiro, S. Marangoni, M. H. Toyama and I. Polikarpov

Synopsis: The atomic resolution crystal structure of neurotoxin Ts1 has been determined. It provides an explanation for the competition between Ts1 and depressant and excitatory neurotoxins.

PDB reference: 1npi

Online 21 February 2003

 

Acta Cryst. (2003). D59, 416-422  [ doi:10.1107/S0907444902021200 ]

Structural comparison of Escherichia coli L-asparaginase in two monoclinic space groups

M. Sanches, J. A. R. G. Barbosa, R. T. de Oliveira, J. A. Abrahão Neto and I. Polikarpov

Synopsis: The structure of E. coli L-asparaginase has been determined at 1.95  Å resolution in space group C2. The crystal structure is compared with the previously determined P2₁ crystal form.

PDB reference: 1nns

Online 21 February 2003

 

Acta Cryst. (2003). D59, 423-432  [ doi:10.1107/S0907444902021911 ]

Crystal structures of r(GGUCACAGCCC)₂

V. Kacer, S. A. Scaringe, J. N. Scarsdale and J. P. Rife

Synopsis: The structure of r(GGUCACAGCCC)₂ has been determined by X-ray crystallography under a variety of conditions. Crystals of this homoduplex were grown from a mixture of two RNAs which were expected to form a heteroduplex in solution. Given the types and number of mismatches, it is not expected that the observed homoduplex would exist in any appreciable amount in solution, yet it is clearly stable in the crystalline form despite a large number of mismatched base pairs.

PDB references: 1kd3, 1kd4 and 1kd5

Online 21 February 2003

 

Acta Cryst. (2003). D59, 433-437  [ doi:10.1107/S0907444902022655 ]

Crystal structure of rGd(CGCGCG): a Z-DNA hexamer duplex with a 5'-(rG) overhang

B. Pan and M. Sundaralingam

Synopsis: The replacement of rG in the 5' terminus of d(GCGCGCG) does not change either the helical form or the water-structure motifs of the helix.

PDB reference: 1m6r

Online 21 February 2003

 

Acta Cryst. (2003). D59, 438-446  [ doi:10.1107/S0907444902022850 ]

Development of a force field for conditional optimization of protein structures

S. H. W. Scheres and P. Gros

Synopsis: Using the force field presented, conditional optimization of three small protein structures against 2.0  Å observed diffraction data shows a large radius of convergence.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 447-452  [ doi:10.1107/S0907444902023405 ]

Direct experimental observation of the hydrogen-bonding network of a glycosidase along its reaction coordinate revealed by atomic resolution analyses of endoglucanase Cel5A

A. Varrot and G. J. Davies

Synopsis: Atomic resolution structures of the endoglucanase Cel5A have been determined at various stages along the reaction coordinate.

PDB references: 1h2j, 1h11 and 1hf6

Online 21 February 2003

 

Acta Cryst. (2003). D59, 453-465  [ doi:10.1107/S0907444902023387 ]

Structure of native phosphoglucose isomerase from rabbit: conformational changes associated with catalytic function

C. Davies and H. Muirhead

Synopsis: The crystal structure of the native form of phosphoglucose isomerase from rabbit is presented. Its comparison with four previously determined ligand-bound enzymes reveals conformational changes that are associated with catalytic activity.

PDB reference: 1n8t

Online 21 February 2003

 

Acta Cryst. (2003). D59, 466-473  [ doi:10.1107/S0907444902023399 ]

Combinatorial crystallization of an RNA-protein complex

D. B. Hoggan, J. A. Chao, G. S. Prasad, C. D. Stout and J. R. Williamson

Synopsis: A combinatorial approach was used to identify the optimal lengths of RNA components that provide high-quality crystals of a ribonucleoprotein complex. The technique was applied to the crystallization of the the S. cerevisiae ribosomal protein L30 in complex with the autoregulatory structure in its cognate mRNA.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 474-482  [ doi:10.1107/S0907444902023685 ]

The structure of T₆ human insulin at 1.0  Å resolution

G. D. Smith, W. A. Pangborn and R. H. Blessing

Synopsis: At 120  K, four residues at the N-terminus of the B chain of monomer 2 undergo a displacement of up to 8  Å.

PDB reference: 1mso

Online 21 February 2003

 

Acta Cryst. (2003). D59, 483-491  [ doi:10.1107/S0907444902023570 ]

Automated tracing of electron-density maps of proteins

T. J. Oldfield

Synopsis: Methods are presented that describe the placement of an C trace into a protein electron-density map without user intervention.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 492-498  [ doi:10.1107/S0907444903000064 ]

Phasing at high resolution using Ta₆Br₁₂ cluster

S. Banumathi, M. Dauter and Z. Dauter

Synopsis: The Ta₆Br cluster compound is a mild derivatization reagent which provides very potent phasing at high resolution owing to its strong anomalous scattering signal.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 499-501  [ doi:10.1107/S0907444902022539 ]

Crystallization and preliminary X-ray diffraction studies of two different crystal forms of the lipase 2 isoform from the yeast Candida rugosa

J. M. Mancheño, M. A. Pernas, M. L. Rúa and J. A. Hermoso

Synopsis: Lipase 2 from the yeast C. rugosa has been crystallized by the hanging-drop vapour-diffusion method under two different experimental conditions, revealing two different oligomeric states of the enzyme. Native data sets have been collected to resolutions of 2.65 and 1.97  Å.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 502-505  [ doi:10.1107/S0907444903000027 ]

Purification and crystallization of the N-terminal domain from the human doublecortin-like kinase

M. H. Kim, U. Derewenda, Y. Devedjiev, Z. Dauter and Z. S. Derewenda

Synopsis: Three different fragments of human doublecortin-like kinase have been purified. The N-terminal domain has been crystallized and preliminary X-ray data have been obtained.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 506-508  [ doi:10.1107/S0907444902021984 ]

Crystallization and preliminary crystallographic analysis of extracellular fragment X3 of YWK-II/APPH: a human sperm membrane protein related to the Alzheimer A4-amyloid precursor protein

M. Yang, W. Zhou, X. Zhang, W. Hu, F. Gao, H. Pang, S. Miao, L. Wang and Z. Rao

Synopsis: Partial extracellular fragment of YWK-II protein/APPH related to the Alzheimer A4-amyloid precursor protein has been expressed in E. coli, crystallized and a complete selenomethionine (Se-Met) derivative data set has been collected at 2.38  Å resolution at ESRF.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 509-511  [ doi:10.1107/S0907444903000052 ]

Crystallization and initial X-ray diffraction of BtuB, the integral membrane cobalamin transporter of Escherichia coli

D. P. Chimento, A. K. Mohanty, R. J. Kadner and M. C. Wiener

Synopsis: The outer-membrane cobalamin transporter BtuB from E. coli has been overexpressed and purified. Trigonal crystals have been obtained of the apo and substrate-bound forms. A native data set has been collected to 2.0  Å using synchrotron radiation.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 512-514  [ doi:10.1107/S0907444902022618 ]

Crystallization and preliminary X-ray crystallographic analysis of SP1, a novel chaperone-like protein

W. Wang, O. Dgany, O. Dym, A. Altman, O. Shoseyov and O. Almog

Synopsis: SP1, a boiling-stable and stress-related protein with no significant sequence homology to other stress-related proteins or small heat-shock proteins, has been crystallized. Diffraction studies of SP1 crystals at low temperature indicated that they belong to the I422 space group, with unit-cell parameters a = 89, b = 89, c = 187  Å.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 515-518  [ doi:10.1107/S0907444902022679 ]

Purification, crystallization and preliminary X-ray diffraction studies of recombinant class A non-specific acid phosphatase of Salmonella typhimurium

R. D. Makde, V. Kumar, A. S. Rao, V. S. Yadava and S. K. Mahajan

Synopsis: The nucleotide sequence of the phoN gene of a pathogenic strain of S. enterica sv. Typhimurium, which encodes a non-specific acid phosphatase, exactly matched that of S. enterica sv. Typhi. The PhoN protein of this strain crystallized in a functionally active form in space groups C2 and C222₁.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 519-521  [ doi:10.1107/S0907444902022904 ]

Crystallization and preliminary crystallographic studies of juvenile hormone-binding protein from Galleria mellonella haemolymph

R. Kolodziejczyk, M. Kochman, G. Bujacz, P. Dobryszycki, A. Ozyhar and M. Jaskolski

Synopsis: Juvenile hormone-binding protein isolated from haemolymph of the wax moth G. mellonella has been crystallized in two polymorphic forms. The trigonal crystals, grown at neutral pH, with space group P3₁21 (or P3₂21) and three molecules in the asymmetric unit, diffract X-rays to 2.7  Å resolution.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 522-525  [ doi:10.1107/S0907444902022916 ]

Crystallization and preliminary crystallographic studies of mung bean cytokinin-specific binding protein

G. Bujacz, O. Pasternak, Y. Fujimoto, Y. Hashimoto, M. M. Sikorski and M. Jaskolski

Synopsis: Recombinant mung bean cytokinin-specific binding protein has been overexpressed in E. coli and crystallized in complex with zeatin, a plant growth hormone. The crystals belong to the hexagonal system, space group P6₂ or P6₄, with multiple copies of the protein in the asymmetric unit, and diffract X-rays to 1.25  Å resolution.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 526-528  [ doi:10.1107/S0907444902023119 ]

Preliminary crystallographic studies of glycogen synthase from Agrobacterium tumefaciens

M. E. Guerin, A. Buschiazzo, J. E. Ugalde, R. A. Ugalde and P. M. Alzari

Synopsis: Glycogen synthase is the enzyme that catalyzes the last step in glycogen biosynthesis. Crystals of the enzyme from the bacterium A. tumefaciens have been obtained that diffract to 2.6  Å resolution and belong to space group P2₁. Selenomethionine has been successfully incorporated, setting the framework for a multiwavelength anomalous diffraction approach.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 529-531  [ doi:10.1107/S0907444902020747 ]

Crystallization and preliminary X-ray analysis of an alkaline serine protease from Nesterenkonia sp.

S. Bakhtiar, J. Vévodová, R. Hatti-Kaul and X.-D. Su

Synopsis: A subtilisin-like serine protease from a novel alkaliphilic bacterium, Nesterenkonia sp. AL20, has been crystallized with space group R3 and unit-cell parameters a = b = 92.26, c = 137.88  Å. High-resolution data have been collected to 1.39  Å.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 532-534  [ doi:10.1107/S0907444902023478 ]

Expression, purification, crystallization and preliminary crystallographic analysis of Trypanosoma brucei phosphofructokinase

J. W. Keillor, C. Lherbet, R. Castonguay, D. Lapierre, J. Martinez-Oyanedel, L. A. Fothergill-Gilmore and M. D. Walkinshaw

Synopsis: The eukaryotic ATP-dependent phosphofructokinase from T. brucei has been overexpressed, purified and crystallized as the apoenzyme and in complex with ligands.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 535-537  [ doi:10.1107/S090744490202348X ]

Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula × tremuloides

P. Johansson, S. Denman, H. Brumer, Å. M. Kallas, H. Henriksson, T. Bergfors, T. T. Teeri and T. A. Jones

Synopsis: The potential cell-wall modifying enzyme xyloglucan endotransglycosylase, XET16A, from Populus tremula × tremuloides has been crystallized. Diffraction data were collected from two different crystal forms belonging to space groups P3₁21/P3₂21 and P6₃.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 538-540  [ doi:10.1107/S0907444902023491 ]

Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion

J. J. B. Cockburn, J. K. H. Bamford, J. M. Grimes, D. H. Bamford and D. I. Stuart

Synopsis: Crystals of bacteriophage PRD1, a virus containing an internal lipid bilayer, have been grown in thin-walled quartz capillary tubes by vapour diffusion as a means of eliminating mechanical handling of the crystals during data collection.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 541-543  [ doi:10.1107/S0907444902023648 ]

Crystallization and preliminary X-ray analysis of swine vesicular disease virus (SVDV)

M. A. Jimenez-Clavero, V. Ley, I. Fita and N. Verdaguer

Synopsis: The crystallization of swine vesicular disease virus (SVDV), isolate SPA/2/'93, in three crystal forms and the arrangement of the viral particles within the different unit cells is described.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 544-546  [ doi:10.1107/S0907444903000039 ]

Purification, crystallization and preliminary X-ray analysis of Caenorhabditis elegans ubiquitin-conjugation enzyme M7.1

J. A. Gavira, D. P. Claxton, J. H. Wang, D. Toh, E. J. Meehan and E. L. DiGiammarino

Synopsis: Here, the cloning, expression, purification and crystallization, as well as preliminary X-ray analysis of C. elegans ubiquitin conjugation enzyme M7.1 is reported.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 547-550  [ doi:10.1107/S0907444902023375 ]

Purification, N-terminal sequencing, partial characterization, crystallization and preliminary crystallographic analysis of two glycosylated serine proteinases from Agkistrodon acutus venom

Z. Zhu, P. Gong, M. Teng and L. Niu

Synopsis: Purified from the venom of A. acutus, two glycosylated snake venom serine proteinases with high homology of N-terminal amino-acid sequences and distinct carbohydrate contents have been crystallized. Two sets of X-ray diffraction data with the resolutions of 2.3 and 2.1  Å, respectively, have been obtained from the two protein crystals.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 551-553  [ doi:10.1107/S0907444903000106 ]

Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8

M. Goto, R. Omi, J. Hoseki, N. Nakagawa, I. Miyahara and K. Hirotsu

Synopsis: A recombinant form of the CTP synthetase from T. thermophilus HB8 has been expressed in E. coli, purified and crystallized. The crystals belong to space group I222 and diffraction data have been collected to 2.25  Å resolution.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 554-557  [ doi:10.1107/S090744490300043X ]

Coexpression, purification, crystallization and preliminary X-ray characterization of glycine decarboxylase (P-protein) of the glycine-cleavage system from Thermus thermophilus HB8

T. Nakai, N. Nakagawa, N. Maoka, R. Masui, S. Kuramitsu and N. Kamiya

Synopsis: The chains of the tetrameric ₂₂ P-protein (200  kDa) have been coexpressed in E. coli, purified and crystallized as a stable complex. A complete native data set has been collected to 2.4  Å resolution.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 558-560  [ doi:10.1107/S0907444903000441 ]

Expression, purification and preliminary crystallographic analysis of human sorbitol dehydrogenase

C. Darmanin, T. Iwata, D. A. Carper, L. G. Sparrow, R.P.-T. Chung and O. El-Kabbani

Synopsis: Crystals of human sorbitol dehydrogenase were grown from polyethylene glycol solutions and diffracted to 2.75  Å resolution.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 561-562  [ doi:10.1107/S0907444903000453 ]

Crystallization and preliminary X-ray crystallographic studies of phosphopantetheine adenylyltransferase from Helicobacter pylori

S. J. Eom, H. J. Ahn, H. W. Kim, S.-H. Baek and S. W. Suh

Synopsis: Phosphopantetheine adenylyltransferase from H. pylori has been crystallized. X-ray diffraction data have been collected to 2.00  Å resolution.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 563-565  [ doi:10.1107/S0907444903000076 ]

Crystallization and preliminary X-ray analysis of the Mj0684 gene product, a putative aspartate aminotransferase, from Methanococcus jannaschii

J. K. Yang, C. Chang, S.-J. Cho, J. Y. Lee, Y. G. Yu, S. H. Eom and S. W. Suh

Synopsis: A putative aspartate aminotransferase (Mj0684 gene product) from M. jannaschii has been crystallized at 296  K. The crystals diffract to 2.2  Å resolution using Cu  K radiation.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 566-568  [ doi:10.1107/S0907444903000088 ]

Crystallization and preliminary X-ray crystallographic studies of salt-tolerant glutaminase from Micrococcus luteus K-3

P. Chantawannakul, K. Yoshimune, Y. Shirakihara, A. Shiratori, M. Wakayama and M. Moriguchi

Synopsis: The salt-tolerant glutaminase from M. luteus K-3 has been crystallized by the hanging-drop vapour-diffusion method and preliminary characterization of the crystals is reported.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 569-571  [ doi:10.1107/S090744490300009X ]

Crystallization and preliminary X-ray crystallographic studies of chorismate synthase from Helicobacter pylori

H. J. Ahn, J. K. Yang, B. I. Lee, H.-J. Yoon, H. W. Kim and S. W. Suh

Synopsis: Tetragonal crystals of chorismate synthase from H. pylori have been obtained (I4, a = b = 145.79, c = 130.98  Å). X-ray diffraction data have been collected to 2.5  Å resolution.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 572-575  [ doi:10.1107/S0907444903000416 ]

Crystallization and preliminary analysis of native and N-terminal truncated isoforms of toluene-4-monooxygenase catalytic effector protein

A. M. Orville, J. M. Studts, G. T. Lountos, K. H. Mitchell and B. G. Fox

Synopsis: These crystallographic results are the first from any member of the catalytic effector proteins, which are essential components of the large and functionally diverse family of multicomponent monooxygenases. The manuscript documents X-ray diffraction data collected from the native, Se-Met enriched and mutant isoforms.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 576-579  [ doi:10.1107/S0907444903000428 ]

Crystallization of a member of the recFOR DNA repair pathway, RecO, with and without bound oligonucleotide

S. Aono, T. Hartsch and U. Schulze-Gahmen

Synopsis: RecFOR proteins are important for DNA repair by homologous recombination in bacteria. The RecO protein from Thermus thermophilus has been cloned and purified, and its binding to oligonucleotides was characterized.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 580-583  [ doi:10.1107/S0907444903001045 ]

Crystallization and preliminary X-ray characterization of cytochrome c'' from the obligate methylotroph Methylophilus methylotrophus

F. J. Enguita, L. Rodrigues, M. Archer, L. Sieker, A. Rodrigues, E. Pohl, D. L. Turner, H. Santos and M. A. Carrondo

Synopsis: Cytochrome c'' from the obligate methylotroph M. methylotrophus has been crystallized under conditions involving the utilization of cadmium chloride as an additive. Addition of glycerol to the crystallization buffer produced crystals suitable for X-ray diffraction, with a reduced solvent content and mosaicity, which diffracted to a maximum resolution of 1.19  Å using synchrotron radiation.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 584-586  [ doi:10.1107/S0907444903001094 ]

Crystallization and preliminary X-ray analysis of candoxin, a novel reversible neurotoxin from the Malayan krait Bungarus candidus

P. Paaventhan, J. S. Joseph, S. Nirthanan, G. Rajaseger, P. Gopalakrishnakone, R. M. Kini and P. R. Kolatkar

Synopsis: Candoxin, a novel reversible neurotoxin from the Malayan krait B. candidus, was crystallized in space group P6₂22 with one molecule per asymmetric unit. The crystals diffracted to 1.8  Å.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 587-590  [ doi:10.1107/S0907444903001057 ]

Crystallization and preliminary X-ray crystallographic analysis of the trimer core from measles virus fusion protein

J. Zhu, Y. Ding, F. Gao, T. Wu, C.W.-H. Zhang, P. Tien, Z. Rao and G. F. Gao

Synopsis: Crystals of two heptad-repeat (HR) helix bundles of measles virus fusion protein were obtained both in native and SeMet-substituted forms. The crystals diffract X-rays to beyond 2  Å resolution.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 591-594  [ doi:10.1107/S0907444903001513 ]

Crystallization and preliminary X-ray diffraction studies of a mosquito-larvicidal toxin from Bacillus thuringiensis subsp. israelensis

P. Boonserm, D. J. Ellar and J. Li

Synopsis: The activated fragment of Cry4B endotoxin from B. thuringiensis subsp. israelensis, which is specifically toxic to mosquito larvae, has been purified and crystallized. The crystals belonged to space group R32 and diffracted to 1.75  Å resolution.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 595-596  [ doi:10.1107/S0907444903001525 ]

Crystallization and preliminary X-ray study of human dipeptidyl peptidase IV (DPPIV)

H. Hiramatsu, K. Kyono, H. Shima, C. Fukushima, S. Sugiyama, K. Inaka, A. Yamamoto and R. Shimizu

Synopsis: Human dipeptidyl peptidase IV has been expressed in the baculovirus system, purified and crystallized.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 597-599  [ doi:10.1107/S0907444902023466 ]

The production, purification and crystallization of a pocilloporin pigment from a reef-forming coral

T. Beddoe, M. Ling, S. Dove, O. Hoegh-Guldberg, R. J. Devenish, M. Prescott and J. Rossjohn

Synopsis: This report details the production, purification, crystallization and preliminary diffraction analysis of a blue-coloured pigment from coral.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 600-602  [ doi:10.1107/S0907444903002075 ]

Using rational screening and electron microscopy to optimize the crystallization of succinate:ubiquinone oxidoreductase from Escherichia coli

R. Horsefield, V. Yankovskaya, S. Törnroth, C. Luna-Chavez, E. Stambouli, J. Barber, B. Byrne, G. Cecchini and S. Iwata

Synopsis: Crystals of SQR that diffract to 2.6  Å were obtained by rational screening and sample quality analysis using electron microscopy.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 603-606  [ doi:10.1107/S0907444902017870 ]

The dual role of CHAPS in the crystallization of stromelysin-3 catalytic domain

A.-L. Gall, M. Ruff and D. Moras

Synopsis: How CHAPS helped crystallization: masking an hydrophobic patch on the protein surface and stabilizing a flexible loop.

Online 21 February 2003

 

Acta Cryst. (2003). D59, 607-610  [ doi:10.1107/S090744490202365X ]

Structure of a tetragonal crystal form of Escherichia coli 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

L. E. Kemp, C. S. Bond and W. N. Hunter

Synopsis: The structure of E. coli 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase in a tetragonal crystal form is presented. Sequence and structure comparisons indicate that the E. coli protein is representative of the enzyme from a number of pathogenic bacteria and is suitable for use in the development of broad-spectrum antibiotics.

PDB reference: 1h3m

Online 21 February 2003

 

Acta Cryst. (2003). D59, 611-614  [ doi:10.1107/S0907444902023661 ]

The organization of divalent cations in the active site of cadmium Escherichia coli fructose-1,6-bisphosphate aldolase

D. R. Hall, L. E. Kemp, G. A. Leonard, K. Marshall, A. Berry and W. N. Hunter

Synopsis: The structure of E. coli class II fructose-1,6-bisphosphate aldolase reconstituted with and crystallized in the presence of Cd²⁺ instead of Zn²⁺ has been determined. The structure shows both a catalytic and structural divalent cation in the active site at positions consistent with those observed when the zinc enzyme is complexed with a transition-state analogue.

PDB reference: 1gyn

Online 21 February 2003

 

Acta Cryst. (2003). D59, 615-617  [ doi:10.1107/S090744490300163X ]

Sheldrick's 1.2  Å rule and beyond

R. J. Morris and G. Bricogne

Synopsis: A structural interpretation of Sheldrick's 1.2  Å rule is presented in terms of sinc-function contributions to |E|² profiles as a function of resolution and of the interferences between characteristic interatomic distances.

Online 21 February 2003