Biological Crystallography

 

Acta Cryst. (2003). D59, 1117-1123  [ doi:10.1107/S0907444903007959 ]

Apocrustacyanin C₁ crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons

J. Habash, T. J. Boggon, J. Raftery, N. E. Chayen, P. F. Zagalsky and J. R. Helliwell

Synopsis: The electron-density maps of the protein and the bound waters are found to be better overall in the structures of apocrustacyanin C₁ studied from a space-grown crystal compared with those from an earth-grown crystal; both were grown using vapour-diffusion crystal-growth geometry. The improved residues are on the surface of the protein, with two involved in or nearby crystal lattice-forming interactions, thus linking an improved crystal-growth mechanism to the molecular level.

PDB references: 1obq and 1obu

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1124-1130  [ doi:10.1107/S0907444903007947 ]

A statistic for local intensity differences: robustness to anisotropy and pseudo-centering and utility for detecting twinning

J. E. Padilla and T. O. Yeates

Synopsis: A new approach is presented for analyzing intensity statistics and for diagnosing perfect hemihedral twinning in macromolecular single-crystal X-ray diffraction data. The new statistic compares pairs of reflections that are close in reciprocal space, making it insensitive to phenomena such as anisotropic diffraction and pseudo-centering which can confound traditional intensity statistics.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1131-1137  [ doi:10.1107/S0907444903008126 ]

A graphical user interface to the CCP4 program suite

E. Potterton, P. Briggs, M. Turkenburg and E. Dodson

Synopsis: A readily extensible system interfacing to the main functionality of the CCP4 program suite and providing project-management and file-viewing tools is presented.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1138-1144  [ doi:10.1107/S0907444903008199 ]

Automated crystallographic system for high-throughput protein structure determination

J. S. Brunzelle, P. Shafaee, X. Yang, S. Weigand, Z. Ren and W. F. Anderson

Synopsis: An automated crystallographic system (ACrS) was developed for high-throughput structure determination. ACrS uses a web user interface, a relational database and a process distributive interface to drive structure determination with well established crystallographic software.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1145-1153  [ doi:10.1107/S0907444903008163 ]

Choice of data-collection parameters based on statistic modelling

A. N. Popov and G. P. Bourenkov

Synopsis: A new method and software program for the optimal choice of data-collection parameters are presented.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1154-1164  [ doi:10.1107/S0907444903008205 ]

Structures of NAD⁺- and NADH-bound 1-L-myo-inositol 1-phosphate synthase

X. Jin and J. H. Geiger

Synopsis: The structure of 1-L-myo-Inositol 1-phosphate synthase in its NAD⁺-bound, NADH-bound and apo forms is presented.

PDB references: 1p1f, 1p1h, 1p1i, 1p1j and 1p1k

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1165-1173  [ doi:10.1107/S0907444903009454 ]

Structures of thymus and activation-regulated chemokine (TARC)

O. A. Asojo, C. Boulègue, D. M. Hoover, W. Lu and J. Lubkowski

Synopsis: Crystal structures of the thymus and activation-regulated chemokine (TARC) have been solved by molecular replacement in both the triclinic P1 and tetragonal P4₁ space groups, and refined to resolutions of 1.72 and 2.1 Å, respectively. Both structural forms and the structure determination are described.

PDB references: 1nr4 and 1nr2

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1174-1182  [ doi:10.1107/S0907444903009922 ]

Improving macromolecular atomic models at moderate resolution by automated iterative model building, statistical density modification and refinement

T. C. Terwilliger

Synopsis: A procedure for iterative model-building, statistical density modification and refinement at moderate resolution (up to about 2.8 Å) is described.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1183-1191  [ doi:10.1107/S0907444903009934 ]

The 1.2 Å structure of the human sulfite oxidase cytochrome b₅ domain

M. J. Rudolph, J. L. Johnson, K. V. Rajagopalan and C. Kisker

Synopsis: The crystal structure of the oxidized human sulfite oxidase cytochrome b₅ domain has been determined at 1.2 Å resolution and has been refined to a crystallographic R factor of 0.107 (R_free = 0.137).

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1192-1199  [ doi:10.1107/S0907444903009958 ]

Flexibility revealed by the 1.85 Å crystal structure of the sliding-clamp subunit of Escherichia coli DNA polymerase III

A. J. Oakley, P. Prosselkov, G. Wijffels, J. L. Beck, M. C. J. Wilce and N. E. Dixon

Synopsis: Structural differences between the two subunits in the unit cell are used to comment on the flexibility of parts of the structure of the ring-shaped sliding clamp.

PDB reference: 1mmi

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1200-1205  [ doi:10.1107/S0907444903010060 ]

Interactions of a new -aminophosphinic derivative inside the active site of TLN (thermolysin): a model for zinc-metalloendopeptidase inhibition

M. Selkti, A. Tomas, J.-F. Gaucher, T. Prangé, M.-C. Fournié-Zaluski, H. Chen and B.-P. Roques

Synopsis: Inhibition of thermolysin by a new -aminophosphinic compound is discussed.

PDB reference: 1os0

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1206-1212  [ doi:10.1107/S0907444903010059 ]

High-resolution structure of human apo dipeptidyl peptidase IV/CD26 and its complex with 1-[({2-[(5-iodopyridin-2-yl)amino]-ethyl}amino)-acetyl]-2-cyano-(S)-pyrrolidine

C. Oefner, A. D'Arcy, A. Mac Sweeney, S. Pierau, R. Gardiner and G. E. Dale

Synopsis: The high-resolution structure of human DPP-IV as an apo enzyme and in complex with a cyano-pyrrolidine inhibitor is reported. The inhibitor forms a covalent intermediate with the active-site serine.

PDB reference: 1pfq

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1213-1218  [ doi:10.1107/S0907444903010515 ]

Structure of a conserved CoA-binding protein synthesized by a cell-free system

T. Wada, M. Shirouzu, T. Terada, Y. Ishizuka, T. Matsuda, T. Kigawa, S. Kuramitsu, S.-Y. Park, J. R. H. Tame and S. Yokoyama

Synopsis: A conserved hypothetical protein, TT1466, from the thermophilic bacterium T. thermophilus HB8 was synthesized by a cell-free system. The crystal structure was highly homologous to that of the CoA-binding domain of succinyl-CoA synthetase and CoA binding of TT1466 was shown by isothermal titration calorimetry.

PDB references: 1iul and 1iuk

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1219-1223  [ doi:10.1107/S0907444903011028 ]

Structure of the hypothetical protein AQ_1354 from Aquifex aeolicus

V. Oganesyan, D. Busso, J. Brandsen, S. Chen, J. Jancarik, R. Kim and S.-H. Kim

Synopsis: Hypothetical protein AQ_1354 appeared to have a fold similar to matrix metalloproteinases, but failed to exhibit such an activity under standard assay conditions.

PDB reference: 1oz9

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1224-1226  [ doi:10.1107/S0907444903008709 ]

Crystallization and preliminary crystallographic study of the outer-membrane lipoprotein receptor LolB, a member of the lipoprotein localization factors

K. Takeda, H. Miyatake, N. Yokota, S. Matsuyama, H. Tokuda and K. Miki

Synopsis: A soluble mutant of the outer-membrane lipoprotein receptor LolB from E. coli was crystallized in two forms. The monoclinic and hexagonal crystals diffracted X-rays to 1.9 and 2.2 Å resolution, respectively, and MAD phasing of monoclinic crystals of the selenomethionylated protein was successful.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1227-1229  [ doi:10.1107/S0907444903010680 ]

Crystallization and preliminary X-ray diffraction analysis of two ribosome-inactivating proteins: lychnin and dianthin 30

S. Fermani, G. Falini, A. Ripamonti, A. Bolognesi, L. Polito and F. Stirpe

Synopsis: Lychnin and dianthin 30 belong to type 1 RIPs. They have been crystallized by the vapour-diffusion method and the crystals diffracted to 1.7 and 1.3 Å, respectively.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1230-1233  [ doi:10.1107/S0907444903007054 ]

Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2

J. A. Hinks, M. Roe, J. C. Y. Ho, F. Z. Watts, J. Phelan, M. McAllister and L. H. Pearl

Synopsis: The BRCT domain from Rhp9/Crb2 has been expressed, purified and crystallized, and preliminary data have been collected.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1234-1237  [ doi:10.1107/S090744490300859X ]

Purification, crystallization and preliminary X-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2

C.-C. Lee, T.-P. Ko, M.-S. Lee, C.-C. Chou, S.-Y. Lai, A.H.-J. Wang and M.-Y. Wang

Synopsis: The rVP2H particles, formed by infectious bursal disease virus (IBDV) outer structural and primary immunogenic protein VP2, have been crystallized and the diffraction data (6 Å) showed that the rVP2H crystals belong to the cubic space group P2₁3 with a unit-cell dimension of 323.1 Å. The icosahedral symmetry of the particles is clearly seen in the self-rotation function maps, with dyads and triads in coincidence with the crystallographic axes.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1238-1240  [ doi:10.1107/S0907444903008606 ]

Crystallization and preliminary X-ray crystallographic analysis of the excisionase-DNA complex from bacteriophage

M. D. Sam, D. Cascio, R. Johnson and R. T. Clubb

Synopsis: In an attempt to understand how Xis controls the directionality of bacteriophage recombination, co-crystals of the DNA-binding domain of Xis in complex with its binding site within the P-arm of the phage have been obtained using the hanging-drop vapor-diffusion method.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1241-1242  [ doi:10.1107/S0907444903008710 ]

Crystallization and preliminary X-ray diffraction analysis of lectin-1 from Pseudomonas aeruginosa

K. Karaveg, Z.-J. Liu, W. Tempel, R. J. Doyle, J. P. Rose and B.-C. Wang

Synopsis: Crystals of native lectin-1 from P. aeruginosa have been obtained and diffract to a resolution beyond 1.9 Å on a rotating-anode X-ray source.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1243-1245  [ doi:10.1107/S0907444903008801 ]

An unfolding/refolding step helps in the crystallization of a poorly soluble protein

G. Kaulmann, G. J. Palm, K. Schilling, R. Hilgenfeld and B. Wiederanders

Synopsis: A novel strategy is presented that leads to the crystallization of the isolated N-terminal propeptide of human procathepsin S, a proteinase belonging to the cathepsin L-like endopeptidases of the clan CA1 cysteine peptidases.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1246-1248  [ doi:10.1107/S0907444903008813 ]

Expression, purification and crystallization of the Plasmodium falciparum enoyl reductase

S. P. Muench, J. B. Rafferty, R. Mcleod, D. W. Rice and S. T. Prigge

Synopsis: An efficient overexpression system for the P. falciparum enoyl reductase facilitated its purification and crystallization. A 2.2 Å resolution diffraction data set was collected.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1249-1252  [ doi:10.1107/S0907444903008825 ]

Crystallization and preliminary X-ray analysis of lectin C from the roots of pokeweed (Phytolacca americana)

M. Hayashida, T. Fujii, M. Hamasu, M. Ishiguro and Y. Hata

Synopsis: Lectin C from the roots of pokeweed (P. americana) was crystallized in the tetragonal and rhombohedral crystal systems. Both crystals were heavily twinned. Use of an additive yielded rhombohedral single crystals diffracting to 1.8 Å resolution.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1253-1255  [ doi:10.1107/S0907444903008837 ]

Crystallization and preliminary X-ray diffraction analysis of the red fluorescent protein eqFP611

K. Nienhaus, B. Vallone, F. Renzi, J. Wiedenmann and G. U. Nienhaus

Synopsis: Red fluorescent protein eqFP611 from the sea anemone Entacmaea quadricolor has been crystallized in space group P6₅22, with unit-cell parameters a = 77.26, b = 77.26, and c = 329.49 Å. The crystals diffracted to 2.5 Å.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1256-1258  [ doi:10.1107/S0907444903008849 ]

Expression, purification, crystallization and preliminary X-ray crystallographic studies of a psychrophilic cellulase from Pseudoalteromonas haloplanktis

S. Violot, R. Haser, G. Sonan, D. Georlette, G. Feller and N. Aghajari

Synopsis: The catalytic core domain of the psychrophilic cellulase CelG from P. haloplanktis has been crystallized and diffraction data have been collected to 1.8 Å.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1259-1261  [ doi:10.1107/S0907444903008795 ]

Cloning, expression, purification and crystallization of the N-domain from the ₂ subunit of the membrane-spanning Na,K-ATPase protein

L. Haue, P. A. Pedersen, P. L. Jorgensen and K. O. Håkansson

Synopsis: The nucleotide-binding domain of Na,K-ATPase was expressed in E. coli, purified and crystallized. Crystals belong to space group F23 and diffract to 3.1 Å.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1262-1264  [ doi:10.1107/S0907444903009041 ]

Crystallization and preliminary crystallographic analysis of a new class of glutathione transferase from nematodes

I. A. Kriksunov, D. J. Schuller, A. M. Campbell, J. Barrett, P. M. Brophy and Q. Hao

Synopsis: X-ray diffraction data from a representative of a new class of GST from H. polygyrus were collected to 1.8 Å resolution at CHESS A1.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1265-1266  [ doi:10.1107/S0907444903009053 ]

Crystallization and preliminary X-ray crystallographic analysis of quinolinate phosphoribosyltransferase of Helicobacter pylori

M.-K. Kim, Y. S. Kim, S.-H. Rho, Y. J. Im, J. H. Lee, G. B. Kang and S. H. Eom

Synopsis: Quinolinate phosphoribosyltransferase from H. pylori was crystallized in three different complexes with quinolinic acid, phthalic acid and nicotinic acid mononucleotide, respectively. These complex crystals were found to belong to space group P4₁2₁2 and diffracted to resolutions of 2.3, 2.8 and 3.3 Å, respectively.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1267-1269  [ doi:10.1107/S0907444903009120 ]

Crystallization and preliminary X-ray analysis of -alanine synthase from the yeast Saccharomyces kluyveri

D. Dobritzsch, Z. Gojkovic, B. Andersen and J. Piskur

Synopsis: -Alanine synthase catalyzes the last step in the pyrimidine catabolic pathway, the formation of -alanine or -aminoisobutyrate from the corresponding N-carbamylated -amino acids. Crystals of this enzyme diffract to at least 2.7 Å and belong to space group P2₁ (unit-cell parameters a  =  117.2, b = 77.1, c = 225.5 Å, = 95.0°).

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1270-1272  [ doi:10.1107/S0907444903009193 ]

Preliminary crystallographic characterization of the human 2 microglobulin His31Tyr mutant in a tetrameric assembly

S. Zuccotti, C. Rosano, P. Mangione, V. Bellotti and M. Bolognesi

Synopsis: A His31Tyr mutant of 2 microglobulin, the light chain of human major histocompatibily complex MHC class I protein, was expressed, purified and crystallized. The isolated crystals display four independent molecules per asymmetric unit and tight local packing.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1273-1275  [ doi:10.1107/S0907444903009442 ]

Crystallization and initial crystal characterization of the N-terminal DH/PH domain of Trio

K. Skowronek, M. Ghumman, Y. Zheng and N. Nassar

Synopsis: The first DH/PH domain of the signal transducer protein Trio has been expressed in E. coli, purified, crystallized and a native data set to 1.7 Å resolution has been collected.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1276-1277  [ doi:10.1107/S0907444903009545 ]

Crystallization and preliminary crystallographic analysis of formyl-CoA tranferase from Oxalobacter formigenes

S. Ricagno, S. Jonsson, N. Richards and Y. Lindqvist

Synopsis: Crystals of formyl-CoA transferase have been grown at room temperature using PEG 4000 as precipitant. The enzyme has been crystallized alone and in presence of CoA: the crystals diffracted to 2.2 and 2.5 Å, respectively.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1278-1279  [ doi:10.1107/S0907444903009569 ]

Crystallization and preliminary X-ray study of -glucosidase from Geobacillus sp. strain HTA-462, one of the deepest sea bacteria

T. Shirai, V. S. Hung, M. Akita, Y. Hatada, S. Ito and K. Horikoshi

Synopsis: An -glucosidase from a Geobacillus strain which was found at the bottom of the Mariana Trench was crystallized in space group C2. The crystal diffracted to 2.5 Å resolution.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1280-1282  [ doi:10.1107/S0907444903009624 ]

Purification, crystallization and preliminary X-ray analysis of an unusual thioredoxin from the gastric pathogen Helicobacter pylori

H. Filson, Á. Fox, D. Kelleher, H. J. Windle and D. A. R. Sanders

Synopsis: This study describes the first crystallization and preliminary X-ray analysis of an unusual thioredoxin (containing an active site of CPDC) from H. pylori. The protein crystallizes in space group P4₁ and diffracts to 2.4 Å resolution.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1283-1284  [ doi:10.1107/S0907444903009843 ]

Crystallization and preliminary crystallographic analysis of the catalytic domain cellobiohydrolase I from Talaromyces emersonii

A. Grassick, G. Birrane, M. Tuohy, P. Murray and T. Higgins

Synopsis: The catalytic domain CBH IB from T. emersonii was crystallized in space group P4₁2₁2, with unit-cell parameters a = b = 74.426, c = 176.920 Å.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1285-1287  [ doi:10.1107/S0907444903009867 ]

New insight into the haemoglobin superfamily: preliminary crystallographic characterization of human cytoglobin

D. de Sanctis, S. Dewilde, A. Pesce, P. Ascenzi, T. Burmester, T. Hankeln, L. Moens and M. Bolognesi

Synopsis: Orthorhombic crystals of cytoglobin, a recently discovered haemoprotein widely distributed in human tissues, have been grown and characterized. Two haem Fe sites have been located using SAD data.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1288-1290  [ doi:10.1107/S090744490300979X ]

Crystallization and preliminary X-ray analysis of the human vascular adhesion protein-1

Y. Nymalm, H. Kidron, A. Söderholm, L. Viitanen, K. Kaukonen, M. Pihlavisto, D. Smith, T. Veromaa, T. T. Airenne, M. S. Johnson and T. A. Salminen

Synopsis: Human vascular adhesion protein-1 has been crystallized.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1291-1293  [ doi:10.1107/S0907444903009879 ]

Crystallization and preliminary X-ray data investigation of the bacterial enterocin A immunity protein at 1.65 Å resolution

B. Dalhus, L. Johnsen and J. Nissen-Meyer

Synopsis: Crystals of the bacterial enterocin A immunity protein diffract to better than 1.7 Å resolution. The crystals belong to space group C2, probably with two molecules in the asymmetric unit.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1294-1295  [ doi:10.1107/S0907444903009892 ]

Crystallization of gankyrin, an oncoprotein that interacts with CDK4 and the S6b (rpt3) ATPase of the 19S regulator of the 26S proteasome

S. Krzywda, A. M. Brzozowski, R. Al-Safty, R. Welchman, M. Mee, S. Dawson, J. Fujita, H. Higashitsuji, R. J. Mayer and A. J. Wilkinson

Synopsis: Gankyrin, an oncoprotein that interacts with the cyclin D-dependent protein kinase CDK4 and the S6b ATPase subunit of the regulator of the proteasome, has been crystallized and native data to 2.1 Å spacing have been collected using synchrotron radiation.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1296-1298  [ doi:10.1107/S0907444903009909 ]

Crystallization and preliminary X-ray diffraction analysis of post-fusion six-helix bundle core structure from Newcastle disease virus F protein

P.-Y. Li, J. Zhu, B. Wu, F. Gao, P. Tien, Z. Rao and G. F. Gao

Synopsis: Crystals of the six-helix bundle core structure from Newcastle disease virus F protein were obtained in two different space groups, P2₁ and C₂.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1299-1301  [ doi:10.1107/S0907444903009776 ]

Purification, crystallization and preliminary X-ray analysis of a Sco1-like protein from Bacillus subtilis, a copper-binding protein involved in the assembly of cytochrome c oxidase

I. Imriskova-Sosova, Q. Ye, B. C. Hill and Z. Jia

Synopsis: Crystals of the soluble domain of the Sco homolog BsSco have been obtained and diffract to 2.3 Å.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1302-1305  [ doi:10.1107/S090744490300982X ]

Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2 domain of Candida tropicalis peroxisomal multifunctional enzyme type 2

M. K. Koski, A. M. Haapalainen, J. K. Hiltunen and T. Glumoff

Synopsis: 2-Enoyl-CoA hydratase 2 domain of C. tropicalis multifunctional enzyme type 2, involved in the peroxisomal -oxidation, has been produced, purified and crystallized. Full MAD data and an additional high-resolution data set were collected from a single SeMet crystal to 2.25 and 1.90 Å resolution, respectively.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1306-1309  [ doi:10.1107/S0907444903010096 ]

Crystallization and preliminary X-ray analysis of an anti-LewisX Fab fragment with and without its LewisX antigen

A. M. van Roon, N. S. Pannu, C. H. Hokke, A. M. Deelder and J. P. Abrahams

Synopsis: A Fab fragment of anti-LewisX murine monoclonal antibody 291-2G3-A has been crystallized using the sitting-drop vapour-diffusion method. X-ray data sets have been collected for the native Fab fragment to 2.05 Å resolution and for the Fab fragment soaked with LewisX to 3.2 and 1.8 Å resolution.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1310-1312  [ doi:10.1107/S090744490301014X ]

Crystallization and X-ray analysis of a bacterial non-haem iron-containing phenylalanine hydroxylase from the Gram-negative opportunistic pathogen Pseudomonas aeruginosa

F. Ekström, G. Stier, J. T. Eaton and U. H. Sauer

Synopsis: The phenylalanine hydroxylase from P. aeruginosa (PaPheOH) was cloned and expressed in E. coli. Crystals grew in space group P6₁ and diffracted to 2.0 Å. The structure determination is in progress using MAD data collected around the Se K edge.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1313-1316  [ doi:10.1107/S0907444903010369 ]

Expression, purification, crystallization and preliminary crystallographic analysis of Leishmania mexicana phosphoglycerate mutase

B. Poonperm, D. G. Guerra, I. W. McNae, L. A. Fothergill-Gilmore and M. D. Walkinshaw

Synopsis: The 2,3-bisphosphoglycerate-independent phosphoglycerate mutase from L. mexicana has been overexpressed, purified and co-crystallized with substrate and product.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1317-1319  [ doi:10.1107/S0907444903010485 ]

Preliminary X-ray crystallographic studies of yeast Hsp40 Ydj1 complexed with its peptide substrate

J. Li and B. Sha

Synopsis: The mechanism by which Hsp40 functions as a molecular chaperone is not understood. To investigate the mechanism, the yeast type I Hsp40 protein Ydj1 C-terminal peptide-binding fragment has been crystallized complexed with its peptide substrate.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1320-1322  [ doi:10.1107/S090744490301062X ]

Cloning, expression, crystallization and preliminary X-ray analysis of the DNA-binding protein Sso10a from Sulfolobus solfataricus

M. J. Teale, M. Kahsai, S. K. Singh, S. P. Edmondson, R. Gupta, J. W. Shriver and E. J. Meehan

Synopsis: The gene for the DNA-binding protein Sso10a from the hyperthermophilic archaeon S. solfataricus was cloned and overexpressed in Escherichia coli. Crystals of the purified protein have been grown that diffract to beyond 2.15 Å resolution.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1323-1326  [ doi:10.1107/S0907444903010692 ]

Expression, purification, crystallization of fragments from the C-terminal region of DFF45/ICAD

H. Ding, S. Qiu, S. Li, J. Symersky, T. J. Green and M. Luo

Synopsis: DFF45 (also known as ICAD) is involved in nuclei DNA fragmentation in apoptosis. Crystals of a key domain in DFF45 were obtained with space group P4_n22 and unit-cell parameters a = b = 162.5, c = 113.0 Å.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1327-1329  [ doi:10.1107/S0907444903010631 ]

Archaeal ADP-dependent phosphofructokinase: expression, purification, crystallization and preliminary crystallographic analysis

J.-J. Jeong, S. Fushinobu, S. Ito, H. Shoun and T. Wakagi

Synopsis: The crystallization and preliminary crystallographic analysis of the ADP-dependent phosphofructokinase of T. litoralis is reported.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1330-1332  [ doi:10.1107/S0907444903010710 ]

Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen

R. Donadini, M. Wåhlberg, T. Kohsaka, Y. Ito and B. A. Fields

Synopsis: YPM, a superantigen from Y. pseudotuberculosis, has been crystallized. X-ray diffraction data were collected to 1.8 Å using synchrotron radiation.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1333-1335  [ doi:10.1107/S0907444903008187 ]

Improving quality and harvest period of protein crystals for structure-based drug design: effects of a gel and a magnetic field on bovine adenosine deaminase crystals

T. Kinoshita, M. Ataka, M. Warizaya, M. Neya and T. Fujii

Synopsis: The overall quality and harvest period of adenosine deaminase was improved in two environments: crystallization with agarose gel and crystallization in a high magnetic field.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1336-1338  [ doi:10.1107/S0907444903009211 ]

X-ray crystallographic study of the possible binding sites of the monovalent cations in the Z-DNA structure

X. Dong

Synopsis: Binding sites of the monovalent cations in the DNA structure are studied by the detection of the rubidium ions in the Z-DNA crystal. The study indicates most rubidium cation sites located in the Z-DNA structure are cation-water hybrid positions.

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1339-1342  [ doi:10.1107/S0907444903011491 ]

Effect of mutations in the T1.5 loop of pectate lyase A from Erwinia chrysanthemi EC16

S. J. Dehdashti, C. N. Doan, K. L. Chao and M. D. Yoder

Synopsis: Using site-directed mutagenesis techniques, four residues in the T1.5 loop of pectate lyase A were mutated to the homologous sequence in pectate lyase E. Enzymatic characterization and structural analysis of the mutant revealed the significance of the T1.5 loop in the cleavage process of pectic enzymes.

PDB references: 1ooc and 1pe9

Online 27 June 2003

 

Acta Cryst. (2003). D59, 1343-1346  [ doi:10.1107/S0907444903009430 ]

Using natural seeding material to generate nucleation in protein crystallization experiments

A. D'Arcy, A. Mac Sweeney and A. Haber

Synopsis: The use of horse hair in protein crystallization has been investigated.

Online 27 June 2003