issue contents

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047

August 2003 issue

Highlighted illustration

Cover illustration: The packing diagram for histone octamers in crystals having space group P65, viewed down the c axis. The packing results from interactions between the interpenetrating left-hand-helical arrays of the octamers (p. 1395).

research papers


link to html
The strengths of intermolecular contacts were calculated for polymorphous lysozyme crystals. The surface energies were calculated to correlate with morphology.

link to html
The crystal structure of cod uracil-DNA glycosylase determined at 1.9 Å resolution reveals a decreased number of ion-pair interactions, a less optimally packed core and an optimization of the electrostatic surface potential of the DNA-binding regions compared with the human counterpart.

link to html
The crystal structure of β-luffin at 2.0 Å resolution has been solved by the molecular-replacement method using polyalanyl trichosanthin as the search model.

link to html
The three-dimensional `fast rotational matching' method is extended to the full six-dimensional (rotation and translation) matching scenario between two three-dimensional objects. The approach allows treatment of five of the six degrees of freedom by means of fast Fourier transforms.

link to html
The crystal structure of a DNA/RNA chimera (dC)(rC)d(ATCG) complexed with daunomycin adopts the B-form helical conformation, similar to the DNA counterpart duplex. A model of B-form RNA duplex has been suggested, in which the interaction of the 2′-hydroxyl groups depends on their 3′-side nucleotide.

link to html
In the absence of an ordered water structure in a crystal air-dried at room temperature, the N-termini of the A chains undergo partial unfolding. Both at room temperature and at 100 K, the majority of the GluB13 side chains in the central core of the hexamer in the air-dried crystal are disordered.

link to html
The structure of the histone-core octamer crystallized in KCl/phosphate compares well with the octamer in the nucleosome but exhibits unique inter-octamer crystal-packing interactions.

link to html
A method for manual crystallization using nanolitre volumes is reported.

link to html
The structure of the LanD protein MrsD at 2.54 Å is described.

link to html
The light-harvesting I complex was found to be most soluble in detergent and amphiphile combinations that also yielded protein crystals. Amphiphiles that were effective for crystallization were found by mass spectrometry to displace bound detergent.

link to html
Low-redundancy SAD data recorded at λ = 2.0 Å on a triiodide derivative of porcine pancreatic elastase shows evidence of radiation damage at iodine heavy-atom sites which can be used very simply within SHARP to enhance the phasing signal.

link to html
The structures of two crystal forms of a four-helix bundle protein have been solved using group–subgroup relations between space groups of polymorphic protein crystals.

link to html
A practical procedure for MAD phasing was successfully performed without the aid of XAFS measurements to solve the crystal structure of the outer-membrane lipoprotein carrier LolA from E. coli.

link to html
The three-dimensional structure of the catalytic domain is reported at 1.78 Å resolution, showing a (β/α)8 barrel fold, and compared with related xylanases.

link to html
The crystal structure of trimeric KDPG aldolase from P. putida, a Class I Schiff-base forming enzyme in the Entner–Doudoroff pathway, is described.

crystallization papers


link to html
A four-copper-containing laccase from C. hirsutus was crystallized. The data were collected to 1.85 Å resolution and initial phases have been obtained using the SAD method.

link to html
The extrinsic protein in an oxygen-evolving complex (OEC23) of photosystem II, tobacco OEC23, was crystallized by the hanging-drop vapour-diffusion technique with PEG 4000 and diffraction data were collected to 2.0 Å.

link to html
LC3, a protein involved in autophagy in mammalian cells, was crystallized. A complete diffraction data set was collected to a resolution limit of 2.1 Å.

link to html
Crystals of the C-terminal truncated domain of the S-layer protein SbsC from G. stearothermophilus have been obtained. Diffraction data were collected to a resolution of 3.0 Å from a native crystal and to 3.2 Å from a Pt derivative.

link to html
Glutathione S-transferase from the human malarial parasite P. falciparum has been crystallized, allowing its X-ray structure determination.

link to html
The carboxylesterase Est30 from B. stearothermophilus has been crystallized in space group C2221, with unit-cell parameters a = 55.83, b = 58.15, c = 179.65 Å. The crystal diffracted to better than 2.0 Å resolution.

link to html
Recombinant protein mavicyanin from C. pepo medullosa, which is a member of the phytocyanin subclass of cupredoxins was crystallizaed and its preliminary crystallographic data were reported.

link to html
Human uridine-cytidine kinase 2 alone or in complex with ligands crystallized in six crystal forms. One form diffracted to 1.8 Å resolution.

link to html
An autoinhibited form of the tandem SH3 domain of p47phox, phagocyte NADPH oxidase regulatory component, has been crystallized and X-ray diffraction data have been collected to 2.1 Å resolution using synchrotron radiation.

link to html
The hibiscus chlorotic ringspot virus (HCRSV), a member of the family Tombusviridae, has been purified and subsequently crystallized in space group P23.

link to html
An intramolecular complex consisting of the interacting domains from two transcriptional regulators, LMO4 and ldb1, has been crystallized. Data were collected to 1.3 Å resolution in addition to MAD data to 1.7 Å resolution.

link to html
Crystals of the fungal immunomodulatory protein Fve from the edible golden needle mushroom (F. velutipes) have been obtained and diffracted to 1.4 Å resolution.

link to html
KsgA, a ubiquitous rRNA dimethyltransferase, has been crystallized in space group C2, with unit-cell parameters a = 173.9, b = 38.4, c = 83.0 Å, β = 90.0°. X-ray data were collected to 1.9 Å with Cu Kα radiation.

link to html
The crystallization of the tetrameric type IIF restriction endonuclease SfiI in complex with DNA is reported.

link to html
Human pirin, a novel highly conserved nuclear protein, has been cloned, expressed, purified and crystallized using PEG as precipitant.

link to html
The crystallization and preliminary X-ray characterization of alginate lyase (family PL-7) from P. aeruginosa are presented.

link to html
Human DJ-1, a protein associated with male fertility and parkinsonism has been crystallized. A data set was collected to 2.00 Å resolution.

link to html
The structural domain of the PRRSV nucleocapsid N protein has been expressed in E. coli and crystallized and a complete native data set has been collected at 2.7 Å resolution.

link to html
A putative dTDP sugar epimerase (NovW) from S. spheroides, implicated in novobiocin biosynthesis, has been overexpressed in E. coli and purified and crystals diffracting X-rays to 2.0 Å resolution have been obtained.

link to html
MurC is responsible for the first step in the addition of the pentapeptide unit of peptidoglycan. Native and SeMet crystals of the E. coli enzyme have been prepared.

link to html
The 36 kDa relaxase domain of the TraI protein of conjugative plasmid F factor has been crystallized by the sitting-drop method in native and selenomethionine-substituted forms. Data sets from trigonal crystals have been collected to 2.5 Å resolution for the native protein and to 3.1 Å resolution for the selenomethionine protein.

link to html
Phosphotransacetylase has been crystallized for the first time. The enzyme from M. thermophila was crystallized in space group I41, with unit-cell parameters a = b = 114.8, c = 127.8 Å, α = β = γ = 90°; the crystals diffracted to 2.5 Å resolution.

short communications


link to html
The C-terminal helix involved in RNA binding has been shown to occlude the binding surface in a previous solution structure of a free U1A. A new 1.8 Å resolution structure of the free protein shows the helix in the same location as in an RNA-bound complex.

link to html
5-Iodo-2′-deoxyuridine (IDU) was the first antiviral nucleoside against herpes simplex virus type 1 and type 2. The structure of d[CACG(IDU)G] reveals two cobalt hexammine complexes in binding modes similar to other cobalt hexammine-containing Z-DNA fragments.

link to html
Conformational change and hydrogen bonding of protein-bound astaxanthin are argued to be the predominant factors responsible for the purple–blue colour of the lobster carapace carotenoprotein β-crustacyanin. The further shifted visible spectra of carotenoids with a cyclopentenedione ring remain to be accounted for.

books received


Acta Cryst. (2003). D59, 1532
doi: 10.1107/S090744490301179X

Acta Cryst. (2003). D59, 1532
doi: 10.1107/S0907444903015890
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds