Biological Crystallography

 

Acta Cryst. (2003). D59, 2051-2052  [ doi:10.1107/S0907444903027069 ]

Acta D ten years on

E. N. Baker and Z. Dauter

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2053-2059  [ doi:10.1107/S090744490302225X ]

Structural analyses of Phycodnaviridae and Iridoviridae

A. A. Simpson, N. Nandhagopal, J. L. Van Etten and M. G. Rossmann

Synopsis: An analysis is presented of pseudo-hexagonal capsomer arrays that make up the structure of very large icosahedral dsDNA viruses.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2060-2064  [ doi:10.1107/S0907444903022261 ]

Crystallization and preliminary analysis of a dsDNA bacteriophage capsid intermediate: Prohead II of HK97

W. R. Wikoff, Z. Che, R. L. Duda, R. W. Hendrix and J. E. Johnson

Synopsis: Prohead II is 50  nm in diameter and is the first assembly intermediate of a dsDNA bacteriophage capsid to be crystallized. The crystals diffract to 5  Å resolution and the crystal packing of this icosahedral early intermediate of HK97 in the P2₁3, 707  Å unit cell is reported.

Online 27 November 2003

    

Acta Cryst. (2003). D59, 2065-2071  [ doi:10.1107/S0907444903025861 ]

Structure of the hybrid cluster protein (HCP) from Desulfovibrio desulfuricans ATCC 27774 containing molecules in the oxidized and reduced states

S. Macedo, D. Aragão, E. P. Mitchell and P. Lindley

Synopsis: Crystals prepared from an old sample of the hybrid cluster protein (HCP) from D. desulfuricans ATCC 27774 contain molecules in both the as-isolated oxidized and reduced states.

PDB reference: 1upx

Online 27 November 2003

    

Acta Cryst. (2003). D59, 2072-2082  [ doi:10.1107/S0907444903025952 ]

Unravelling the structural chemistry of the colouration mechanism in lobster shell

N. E. Chayen, M. Cianci, J. G. Grossmann, J. Habash, J. R. Helliwell, G. A. Nneji, J. Raftery, P. J. Rizkallah and P. F. Zagalsky

Synopsis: Biological crystallography, spectroscopy, solution X-ray scattering and microscopy have been applied to study the molecular basis of the colouration in lobster shell and this article presents a review of this progress, concentrating on recent results but spanning more than 50  years of work.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2083-2087  [ doi:10.1107/S0907444903017682 ]

Direct phasing at low resolution of a protein copurified with human paraoxonase (PON1)

A. Fokine, R. Morales, C. Contreras-Martel, P. Carpentier, F. Renault, D. Rochu and E. Chabriere

Synopsis: First low-resolution direct phasing of a unknown protein with GENMEM, a program based on the topological analysis of the electronic density. A first simple step of a protein structure determination by X-ray crystallography in a tricky case is described.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2088-2093  [ doi:10.1107/S0907444903018092 ]

Resolving the space-group ambiguity of crystals of tomato fruit polygalacturonase

S. Heffron, S. Watkins, R. Moeller, A. H. Taban, R. Butowt, D. DellaPenna and F. Jurnak

Synopsis: The space-group ambiguity for the first plant polygalacturonase to be crystallized, tomato PG2, is discussed and resolved.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2094-2103  [ doi:10.1107/S090744490301833X ]

Reintroducing electrostatics into protein X-ray structure refinement: bulk solvent treated as a dielectric continuum

L. Moulinier, D. A. Case and T. Simonson

Synopsis: With the recent development of accurate implicit solvent models, it is time to test whether electrostatic forces, including the contributions of crystal solvent, should be re-introduced into protein X-ray structure refinements. Using the Generalized Born continuum solvent model, promising results are obtained for three proteins solved at moderate resolutions.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2104-2113  [ doi:10.1107/S0907444903018596 ]

Distance distributions and electron-density characteristics of protein models

P. H. Zwart and V. S. Lamzin

Synopsis: The analytical expression for the coordinate error-dependent distribution of an interatomic distance and a simple relation between the positional error and the map correlation are presented, which can be used in assisting the decision-making process during automated model-building procedures.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2114-2124  [ doi:10.1107/S0907444903018675 ]

Fisher's information in maximum-likelihood macromolecular crystallographic refinement

R. A. Steiner, A. A. Lebedev and G. N. Murshudov

Synopsis: In this account, the use of Fisher's information matrix and the related scoring method of minimization in maximum-likelihood macromolecular crystallographic refinement is discussed. The scoring method has been implemented in the program REFMAC5 from CCP4. The algorithm uses the sparse approximation of the matrix and is computationally very efficient.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2125-2132  [ doi:10.1107/S0907444903018973 ]

Structure of viscotoxin A3: disulfide location from weak SAD data

J. É. Debreczeni, B. Girmann, A. Zeeck, R. Krätzner and G. M. Sheldrick

Synopsis: The structure of viscotoxin A3 was solved by sulfur-SAD phasing using Cu  K radiation and an innovation in the program SHELXD for the direct location of disulfide units.

PDB reference: 1okh

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2133-2139  [ doi:10.1107/S0907444903018900 ]

Three-dimensional atomic structure of a catalytic subunit mutant of human protein kinase CK2

E. Pechkova, G. Zanotti and C. Nicolini

Synopsis: The three-dimensional crystal structure of the triple-point mutant of the catalytic subunit of human protein kinase CK2 has been determined at 2.4  Å resolution. Microcrystals were obtained by a protein-crystallization method based on thin-film nanotechnology and diffraction data were collected by the microfocus beamline at the ESRF synchrotron.

PDB reference: 1na7

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2140-2149  [ doi:10.1107/S0907444903019231 ]

Refined structure of Pyrococcus furiosus ornithine carbamoyltransferase at 1.87  A

J. Massant, J. Wouters and N. Glansdorff

Synopsis: The high-resolution (1.87  Å) structure of dodecameric ornithine carbamoyltransferase from the hyperthermophilic archaeon P. furiosus allows a finer description of the active site and of the interactions between the different interfaces from which the hyperthermostability of the protein emerges.

PDB reference: 1pvv

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2150-2162  [ doi:10.1107/S0907444903019267 ]

Description of protein-DNA complexes in terms of electron-density topological features

A. Becue, N. Meurice, L. Leherte and D. P. Vercauteren

Synopsis: Topological analyses of medium-resolution electron-density maps of macromolecules allow critical point graphs to be obtained which are reduced representations of the structures, including physico-chemical properties. The method has been applied to two protein-DNA complexes, NF-B and HIF-1.

Online 27 November 2003

    

Acta Cryst. (2003). D59, 2163-2168  [ doi:10.1107/S0907444903019620 ]

Structural studies of Proteus mirabilis catalase in its ground state, oxidized state and in complex with formic acid

P. Andreoletti, A. Pernoud, G. Sainz, P. Gouet and H. M. Jouve

Synopsis: The structure of P. mirabilis catalase in its ground state, oxidized state and in complex with an inhibitor, formic acid, has been solved at 2.3  Å resolution.

PDB references: 1m85, 1klw and 1mqf

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2169-2182  [ doi:10.1107/S0907444903019668 ]

Physical and structural studies on the cryocooling of insulin crystals

A. Vahedi-Faridi, J. Lovelace, H. D. Bellamy, E. H. Snell and G. E. O. Borgstahl

Synopsis: The reflection profiles from single-domain and multi-mosaic domain insulin crystals were analyzed using highly monochromatic parallel synchrotron radiation and fine -slicing to help understand the effects of temperature on diffraction quality. Corresponding effects on the insulin structure were re-examined.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2183-2190  [ doi:10.1107/S0907444903019589 ]

The structures of the PII proteins from the cyanobacteria Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803

Y. Xu, P. D. Carr, P. Clancy, M. Garcia-Dominguez, K. Forchhammer, F. Florencio, N. Tandeau de Marsac, S. G. Vasudevan and D. L. Ollis

Synopsis: Crystal structures are presented from two cyanobacterial forms of the protein PII. Their overall structures are remarkably similar to that of other known PII proteins despite the fact that they mediate different biochemical processes, cell responses, and stimulate a different set of receptors.

PDB references: 1qy7 and 1qy7

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2191-2199  [ doi:10.1107/S0907444903019942 ]

Structure of superoxide dismutase from Pyrobaculum aerophilum presents a challenging case in molecular replacement with multiple molecules, pseudo-symmetry and twinning

S. Lee, M. R. Sawaya and D. Eisenberg

Synopsis: The crystal structure of superoxide dismutase from P. aerophilum was determined by molecular replacement at 1.8  Å resolution. Overcoming the difficulties in detecting twinning in the presence of non-crystallographic symmetry is discussed as well as the difficulties in molecular replacement with multiple molecules and pseudo-crystallographic symmetry.

PDB reference: 1p7g

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2200-2210  [ doi:10.1107/S0907444903020316 ]

Effective electron-density map improvement and structure validation on a Linux multi-CPU web cluster: The TB Structural Genomics Consortium Bias Removal Web Service

V. Reddy, S. M. Swanson, B. Segelke, K. A. Kantardjieff, J. C. Sacchettini and B. Rupp

Synopsis: A public web service for effective map improvement, bias removal and structure validation has been implemented on a Linux multi-CPU cluster.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2211-2217  [ doi:10.1107/S0907444903018493 ]

Structure of an insect -class glutathione S-transferase from a DDT-resistant strain of the malaria vector Anopheles gambiae

L. Chen, P. R. Hall, X. E. Zhou, H. Ranson, J. Hemingway and E. J. Meehan

Synopsis: The crystal structure of an insect -class glutathione S-transferase agGSTd1-6 from a DDT-resistant strain of the malaria vector A. gambiae has been determined at a resolution of 2.0  Å.

PDB reference: 1pn9

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2218-2227  [ doi:10.1107/S0907444903020493 ]

Structure of rabbit-muscle glyceraldehyde-3-phosphate dehydrogenase

S. W. Cowan-Jacob, M. Kaufmann, A. N. Anselmo, W. Stark and M. G. Grütter

Synopsis: The crystal structure of rabbit-muscle GAPDH at 2.4  Å, with one NAD⁺ molecule bound to each dimer of the tetrameric enzyme, is the first detailed structure of a mammalian GAPDH and could aid in structure-based drug design.

PDB reference: 1j0x

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2228-2236  [ doi:10.1107/S0907444903020559 ]

Low-temperature water reconstruction in concanavalin A, with implications for controlled protein crystal annealing

S. Parkin and H. Hope

Synopsis: Water reconstruction during a low-temperature phase transition in flash-cooled concanavalin A is described. The phase transition and its implications for controlled annealing of protein crystals are discussed.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2237-2241  [ doi:10.1107/S0907444903020729 ]

Acetobacter turbidans -amino acid ester hydrolase: merohedral twinning in P2₁ obscured by pseudo-translational NCS

T. R. M. Barends and B. W. Dijkstra

Synopsis: The structure of the -amino acid ester hydrolase from A. turbidans has been determined. A description is given of the structure-solution process, which was severely hampered by the simultaneous occurrence of twinning and pseudo-translational symmetry.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2242-2246  [ doi:10.1107/S0907444903020584 ]

Making the most of two crystals: structural analysis of a conserved hypothetical protein using native gel screening and SAD phasing

J. S. Lott, M. J. Banfield, J. Sigrell and E. N. Baker

Synopsis: A protein of unknown function, PAE2307, from the hyperthermophilic archaeon P. aerophilum has been crystallized and gel screening has been used to identify a suitable derivative for SAD phasing, given only two usable crystals.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2247-2250  [ doi:10.1107/S0907444903016421 ]

Purification, crystallization and X-ray diffraction analysis of the extracellular part of the human Fc receptor for IgA, FcRI (CD89)

K. Wenig and P. Sondermann

Synopsis: This study describes the crystallization and X-ray analysis of the extracellular region of the human IgA receptor FcRI. X-ray diffraction data were collected to a limiting resolution of 2.8  Å using synchrotron radiation.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2251-2253  [ doi:10.1107/S0907444903020602 ]

Crystallization and preliminary crystallographic analysis of the extracellular fragment of FcRI/CD89

M. Yang, G. Xu, S. Li, L. Sun, N. Shi, W. Zeng, H. Pang, W. Zhang and Z. Rao

Synopsis: Expression, refolding, crystallization and MAD data collection of the extracellular fragment of FcRI/CD89 is reported.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2254-2256  [ doi:10.1107/S0907444903018742 ]

Crystallization and preliminary X-ray diffraction analysis of Mycobacterium smegmatis Dps

S. Roy, S. Gupta, S. Das, K. Sekar, D. Chatterji and M. Vijayan

Synopsis: The DNA-binding protein from stationary phase cells (Dps) of M. smegmatis has been crystallized in three different crystal forms.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2257-2258  [ doi:10.1107/S0907444903018754 ]

Crystallization of a novel -amylase, AmyB, from the thermophilic halophile Halothermothrix orenii

T. C. Tan, Y. Y. Yien, B. K. C. Patel, B. N. Mijts and K. Swaminathan

Synopsis: The first crystallographic study of an -amylase from an organism that is both thermophilic and halophilic, at 1.97  Å resolution is reported.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2259-2261  [ doi:10.1107/S090744490301895X ]

Expression and crystallographic characterization of the extracellular domain of human natural killer cell triggering receptor NKp46

M. Ponassi, C. Cantoni, R. Biassoni, R. Conte, A. Spallarossa, A. Moretta, L. Moretta, M. Bolognesi and D. Bordo

Synopsis: The extracellular region of NKp46 (22.7  kDa), a natural cytotoxicity receptor composed of two Ig-like domains held to be involved in cellular ligand recognition and binding, has been crystallized in a hexagonal crystal form suitable for a detailed structural investigation.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2262-2264  [ doi:10.1107/S0907444903018948 ]

Crystallization of RusA Holliday junction resolvase from Escherichia coli

T. A. Muranova, S. E. Sedelnikova, P. M. Leonard, A. Pasquo, E. L. Bolt, R. G. Lloyd and J. B. Rafferty

Synopsis: The Holliday junction resolvase enzyme RusA from E. coli has been purified and crystallized. A selenomethionine-incorporated form of the enzyme that diffracts to 2.1  Å resolution has been used to collect a full three-wavelength MAD data set.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2265-2268  [ doi:10.1107/S0907444903018985 ]

Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals from Thermotoga maritima and Escherichia coli

R.-T. Guo, T.-P. Ko, C.-C. Chou, H.-L. Shr, H.-M. Chu, Y.-H. Tsai, P.-H. Liang and A.H.-J. Wang

Synopsis: Crystals suitable for structural analysis of octaprenyl pyrophosphate synthase, a long-chain trans-type prenyltransferase, have been obtained from thermophilic T. maritima and mesophilic E. coli. The structure of the T. maritima enzyme has been solved at 2.28  Å resolution.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2269-2271  [ doi:10.1107/S090744490301970X ]

Crystallization and preliminary crystallographic studies of the C-terminal domain of human FKBP52

B. Wu, P. Li, C. Shu, B. Shen and Z. Rao

Synopsis: The C-terminal domain of human FKBP52 has been crystallized. The crystals diffracted to 2.7  Å.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2272-2274  [ doi:10.1107/S0907444903020031 ]

Purification, crystallization and preliminary X-ray diffraction analysis of S-formylglutathione hydrolase from Arabidopsis thaliana: effects of pressure and selenomethionine substitution on space-group changes

K. E. McAuley, I. Cummins, M. Papiz, R. Edwards and A. P. Fordham-Skelton

Synopsis: S-Formylglutathione hydrolase has been crystallized and several crystallographic data sets have been collected to a maximum resolution of 1.6  Å. The space group changes from monoclinic C2 to trigonal P3₁21 or P3₂21 when the crystal is pressurized in a xenon pressure chamber.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2275-2278  [ doi:10.1107/S0907444903020821 ]

Crystallization and preliminary X-ray analysis of the small component of 4-hydroxyphenylacetate 3-monooxygenase (HpaC) and its cofactor complex from Thermus thermophilus HB8

S.-H. Kim, H. Miyatake, T. Hisano, N. Ohtani and K. Miki

Synopsis: The small component of 4-hydroxyphenylacetate 3-monooxygenase (HpaC) from T. thermophilus HB8 has been crystallized in both apoprotein and flavin-complex forms, and these crystals diffracted to 1.85 and 1.3  Å resolution, respectively.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2279-2282  [ doi:10.1107/S0907444903021036 ]

Bacterial expression, purification and preliminary X-ray crystallographic characterization of the invertase inhibitor Nt-CIF from tobacco

M. Hothorn, F. Bonneau, G. Stier, S. Greiner and K. Scheffzek

Synopsis: The cell-wall invertase inhibitor Nt-CIF from N. tabacum has been expressed in E. coli. Crystallization experiments of the purified protein resulted in four different crystal forms, all diffracting to resolutions higher than 2.5  Å.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2283-2285  [ doi:10.1107/S0907444903019127 ]

Purification, crystallization and preliminary X-ray analysis of a water-soluble chlorophyll protein from Brassica oleracea L. var. acephala (kale)

D. Horigome, H. Satoh and A. Uchida

Synopsis: A water-soluble chlorophyll protein (WSCP) with a chlorophyll a:b ratio of 6:1 from B. oleracea L. var. acephala (kale) was purified and crystallized. A native data set was collected to 2.8  Å resolution at 293  K using Cu  K radiation from a rotating-anode generator.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2286-2288  [ doi:10.1107/S0907444903019140 ]

Expression, crystallization and preliminary X-ray crystallographic studies of Arthrobacter globiformis inulin fructotransferase

M. Momma, Z. Fujimoto, N. Maita, K. Haraguchi and H. Mizuno

Synopsis: A recombinant form of A. globiformis inulin fructotransferase was overexpressed in E. coli, purified to homogeneity and crystallized by the hanging-drop vapour-diffusion technique using lithium sulfate as a precipitant. The crystal diffracted to better than 1.5  Å at 100  K.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2289-2291  [ doi:10.1107/S0907444903019681 ]

Crystallization and preliminary X-ray crystallographic studies of -galactosidase I from Mortierella vinacea

Z. Fujimoto, W.-D. Kim, S. Kaneko, G.-G. Park, M. Momma, H. Kobayashi and H. Mizuno

Synopsis: -Galactosidase I from M. vinacea was crystallized in two tetragonal forms by the hanging-drop vapour-diffusion method.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2292-2293  [ doi:10.1107/S0907444903019826 ]

Crystallization and X-ray diffraction of 5'-fluoro-5'-deoxyadenosine synthase, a fluorination enzyme from Streptomyces cattleya

C. Dong, H. Deng, M. Dorward, C. Schaffrath, D. O'Hagan and J. H. Naismith

Synopsis: The purification and crystallization of an enzyme from S. cattleya which is responsible for the synthesis of the C-F bond during fluoroacetate and 4-fluorothreonine biosynthesis is reported.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2294-2296  [ doi:10.1107/S090744490302002X ]

Crystallization and preliminary X-ray crystallographic studies of NADP-dependent 3-hydroxyisobutyrate dehydrogenase from Thermus thermophilus HB8

N. K. Lokanath, I. Shiromizu, Y. Nodake, M. Sugahara, S. Yokoyama, S. Kuramitsu, M. Miyano and N. Kunishima

Synopsis: Crystals of the 3-hydroxyisobutyrate dehydrogenase from T. thermophilus HB8 have been obtained and diffract to 1.8  Å resolution. Crystals belong to space group P2₁2₁2₁, with a tetramer in the asymmetric unit.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2297-2299  [ doi:10.1107/S0907444903020110 ]

Crystallization and preliminary crystallographic analysis of human serine dehydratase

L. Sun, X. Li, Y. Dong, M. Yang, Y. Liu, X. Han, X. Zhang, H. Pang and Z. Rao

Synopsis: L-Serine dehydratase from human liver was overexpressed in E. coli, purified and crystallized. A complete native data set to 2.5  Å resolution has been collected and structure determination is in progress.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2300-2302  [ doi:10.1107/S0907444903020304 ]

Crystallization and preliminary crystallographic characterization of an SH3 domain from the IB1 scaffold protein

I. Dar, C. Bonny, J. T. Pedersen, M. Gajhede and O. Kristensen

Synopsis: Deliberate protein oxidation has led to bigger crystals and additional crystal forms of the IB1-SH3 domain. The rectangular prism form diffracts to 3.0  Å resolution.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2303-2305  [ doi:10.1107/S0907444903020456 ]

Crystallization and preliminary X-ray diffraction data of Mycobacterium tuberculosis FbpC1 (Rv3803c)

R. A. Wilson, S. Rai, W. N. Maughan, L. Kremer, B. M. Kariuki, K. D. M. Harris, T. Wagner, G. S. Besra and K. Fütterer

Synopsis: The crystallization of M. tuberculosis FbpC1, a secreted protein homologous to the essential antigen 85 complex but of unknown function, is described. Crystals of FbpC1 belong to space group P4₁2₁2 at room temperature and undergo a temperature-induced phase transition to P2₁2₁2₁ upon freezing.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2306-2309  [ doi:10.1107/S0907444903020596 ]

Crystallization and preliminary neutron analysis of the dissimilatory sulfite reductase D (DsrD) protein from the sulfate-reducing bacterium Desulfovibrio vulgaris

T. Chatake, N. Mizuno, G. Voordouw, Y. Higuchi, S. Arai, I. Tanaka and N. Niimura

Synopsis: A large single crystal of DsrD (1.7  mm³) has been successfully crystallized and neutron diffraction data were collected to 2.4  Å resolution.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2310-2312  [ doi:10.1107/S0907444903020626 ]

Purification, partial characterization and crystallization of acucetin, a protein containing both disintegrin-like and cysteine-rich domains released by auto-proteolysis of a P-III-type metalloproteinase AaH-IV from Agkistrodon acutus venom

J. Zang, Z. Zhu, Y. Yu, M. Teng, L. Niu, Q. Huang, Q. Liu and Q. Hao

Synopsis: Acucetin, containing both disintegrin-like and cysteine-rich domains, was purified from the autoproteolytic products of a P-III-type metalloproteinase AaH IV. The N-terminal amino-acid sequence was determined and preliminary crystallographic research was carried out.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2313-2315  [ doi:10.1107/S0907444903020766 ]

Crystallization of CprB, an autoregulator-receptor protein from Streptomyces coelicolor A3(2)

R. Natsume, R. Takeshita, M. Sugiyama, Y. Ohnishi, T. Senda and S. Horinouchi

Synopsis: CprB, an autoregulator-receptor protein derived from S. coelicolor A3(2), was crystallized. MAD data collection at 2.4  Å resolution was carried out at BL41XU, SPring-8.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2316-2318  [ doi:10.1107/S0907444903020961 ]

Crystallization and preliminary X-ray analysis of N-acetyl-1-D-myo-inosityl-2-deoxy--D-glucopyranoside deacetylase (MshB) from Mycobacterium tuberculosis

A. A. McCarthy, R. Knijff, N. A. Peterson and E. N. Baker

Synopsis: The deacetylase MshB from M. tuberculosis (Rv1170), which is involved in the biosynthesis of the key antioxidant mycothiol, has been expressed, purified and crystallized in a form suitable for high-resolution X-ray structural analysis.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2319-2321  [ doi:10.1107/S0907444903021231 ]

Expression, purification, crystallization and preliminary crystallographic analysis of phosphoserine aminotransferase from Bacillus alcalophilus

A. P. Dubnovitsky, E. G. Kapetaniou and A. C. Papageorgiou

Synopsis: Phosphoserine aminotransferase from the obligate alkalophile B. alcalophilus was expressed, purified and crystallized in two different space groups, C222₁ and P2₁2₁2. Crystals diffract to 1.7 and 1.6  Å resolution, respectively.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2322-2324  [ doi:10.1107/S0907444903021279 ]

Crystallization and preliminary X-ray analysis of the glycogen synthase from Pyrococcus abyssi

C. Horcajada, E. Cid, J. J. Guinovart, N. Verdaguer and J. C. Ferrer

Synopsis: Recombinant glycogen synthase from P. abyssi has been crystallized. Crystals diffracted to 3.5  Å resolution.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2325-2327  [ doi:10.1107/S0907444903021267 ]

Crystallization of [Fe₃S₄]-ferredoxin from the hyperthermophile archaeon Pyrococcus furiosus

M. S. Nielsen, P. Harris and H. E. M. Christensen

Synopsis: Crystals of P. furiosus ferredoxin containing an [Fe₃S₄]-cluster have been prepared and X-ray diffraction data have been collected to 1.5  Å.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2328-2331  [ doi:10.1107/S0907444903021565 ]

Crystallization and phasing of alanine dehydrogenase from Archaeoglobus fulgidus

N. Smith, M. Mayhew, H. Robinson, A. Héroux, D. Charlton, M. J. Holden and D. T. Gallagher

Synopsis: Archaeal alanine dehydrogenases (AlaDehyds) belong to a family that includes bacterial ornithine cyclodeaminase, marsupial lens proteins, and a human thyroxine carrier, but are unrelated to bacterial AlaDehyds. Crystals of the dimeric AlaDehyd from A. fulgidus undergo a space group change on derivatization, leading to structure determination by MAD methods.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2332-2333  [ doi:10.1107/S0907444903022741 ]

Crystallization and preliminary X-ray crystallographic analysis of human peptidylarginine deiminase V

K. Arita, H. Hashimoto, T. Shimizu, M. Yamada and M. Sato

Synopsis: Monoclinic crystals of human peptidylarginine deiminase V have been obtained. Native data have been processed at 2.7  Å resolution using the SPring-8 synchrotron-radiation source.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2334-2336  [ doi:10.1107/S0907444903023266 ]

Crystallization and preliminary X-ray crystallographic analysis of the Hsp100 chaperone ClpB from Thermus thermophilus

S. Lee, M. Hisayoshi, M. Yoshida and F. T. F. Tsai

Synopsis: ClpB from T. thermophilus has been crystallized in the presence of adenosine 5'-(,-imido)triphosphate and adenosine 5'-(-thio)triphosphate, respectively. This is the first report of the crystallization of a full-length member of the ClpB/Hsp104 family of molecular chaperones.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2337-2341  [ doi:10.1107/S0907444903018729 ]

Order and disorder in crystals of hexameric NTPases from dsRNA bacteriophages

E. J. Mancini, J. M. Grimes, R. Malby, G. C. Sutton, D. E. Kainov, J. T. Juuti, E. V. Makeyev, R. Tuma, D. H. Bamford and D. I. Stuart

Synopsis: Crystals have been grown of protein P4, the hexameric packaging NTPase, for several dsRNA bacteriophages. Comparison of the packing arrangements of different P4 hexamers and analysis of the disorder provides insights into the, probably biologically relevant, flexibility of this family of proteins.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2342-2344  [ doi:10.1107/S0907444903020730 ]

Structure of d(ITITACAC) complexed with distamycin at 1.6  Å resolution

J. Deng, B. Pan and M. Sundaralingam

Synopsis: The crystal structure of d(ITITACAC) complexed with distamycin illustrates the features of the 2:1 side-by-side binding mode adopted by alternating DNA octamer duplexes.

PDB reference: 1jux

NDB reference: DD0043

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2345-2347  [ doi:10.1107/S0907444903019103 ]

Oils used in microbatch crystallization do not remove a detergent from the drops they cover

T. R. M. Barends and B. W. Dijkstra

Synopsis: A new method is used to show that the detergent dodecyl maltoside does not migrate into the oil in a microbatch experiment and may be safely used in microbatch crystallization experiments.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2348-2352  [ doi:10.1107/S090744490301967X ]

Molecules of Escherichia coli MobB assemble into densely packed hollow cylinders in a crystal lattice with 75% solvent content

S. E. Rangarajan, A. Tocilj, Y. Li, P. Iannuzzi, A. Matte and M. Cygler

Synopsis: The molecules of E. coli MobB, an enzyme involved in the final step of molybdenum cofactor biosynthesis, form intertwined dimers. These dimers assemble into helices, two of which form a hollow cylinder with a diameter of 250  Å and pitch of 98  Å. These cylinders pack into a hexagonal lattice with P6₄22 symmetry and 75% solvent content.

PDB reference: 1p9n

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2353-2355  [ doi:10.1107/S0907444903020699 ]

Crystallization of the bovine ADP/ATP carrier is critically dependent upon the detergent-to-protein ratio

C. Dahout-Gonzalez, G. Brandolin and E. Pebay-Peyroula

Synopsis: The control of detergent-to-protein ratio using Bio-Beads was necessary to obtain crystals that diffract to 2.2  Å resolution. Two crystal forms were obtained, one of which presented a defect that is analyzed herein.

Online 27 November 2003

 

Acta Cryst. (2003). D59, 2356-2358  [ doi:10.1107/S0907444903021784 ]

Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals

T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ringe and G. A. Petsko

Synopsis: Data supporting the application of sodium malonate as a universal cryoprotectant and stabilizing solution for salt-grown protein crystals are presented.

Online 27 November 2003