Biological Crystallography

 

Acta Cryst. (2004). D60, 405-411  [ doi:10.1107/S0907444903026805 ]

Platinum-induced space-group transformation in crystals of the platelet glycoprotein Ib N-terminal domain

K. I. Varughese, Z. M. Ruggeri and R. Celikel

Synopsis: The interaction between platelet glycoprotein (GP) Ib and von Willebrand factor is essential for thrombus formation, leading to the arrest of bleeding. The crystal structure of the N-terminal domain of GP Ib has been solved at 1.7 Å resolution.

PDB reference: 1qyy

Online 25 February 2004

 

Acta Cryst. (2004). D60, 412-421  [ doi:10.1107/S0907444903027938 ]

The role of solvent transport in cryo-annealing of macromolecular crystals

D. H. Juers and B. W. Matthews

Synopsis: Annealing experiments with flash-cooled protein crystals suggest that water is transported into or out of the crystal during the melting phase, depending on the ambient humidity. Measurements of the bulk density of cryosolvents suggest that during successful annealing the water transport modulates the thermal contraction of the cryosolvent in order to compensate for the thermal contraction of the protein crystal lattice.

Online 25 February 2004

    

Acta Cryst. (2004). D60, 422-431  [ doi:10.1107/S0907444903028415 ]

Structures of d(GCGAAGC) and d(GCGAAAGC) (tetragonal form): a switching of partners of the sheared G·A pairs to form a functional G·A×A·G crossing

T. Sunami, J. Kondo, I. Hirao, K. Watanabe, K. Miura and A. Takénaka

Synopsis: The DNA fragments d(GCGAAGC) form a new type of bulge-in duplex with the G·A×A·G crossing in the central part, to which an addition of an A residue induces exchange of the partners of G·A pairs to form a base-intercalated duplex.

PDB references: 1ub8 and 1ue4

Online 25 February 2004

 

Acta Cryst. (2004). D60, 432-438  [ doi:10.1107/S0907444903028956 ]

Likelihood-enhanced fast rotation functions

L. C. Storoni, A. J. McCoy and R. J. Read

Synopsis: Likelihood-enhanced fast rotation functions have been developed and shown to be more sensitive to the correct orientation than the traditional fast rotation functions, especially when information from a partial solution can be exploited.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 439-446  [ doi:10.1107/S090744490302938X ]

Structural insight into nucleotide recognition in tau-protein kinase I/glycogen synthase kinase 3

M. Aoki, T. Yokota, I. Sugiura, C. Sasaki, T. Hasegawa, C. Okumura, K. Ishiguro, T. Kohno, S. Sugio and T. Matsuzaki

Synopsis: The first two crystal structures of binary complexes of full-length glycogen synthase kinase 3 with the ATP analogues hydrolysate ADP and AMPPNP are described. Although several other crystal structures of the enzyme have already been reported, none of them contain ATP analogues. The structures of these binary complexes reveal detailed features of nucleotide recognition by GSK3 and may have useful implications in the design of drug candidates against Alzheimer's disease.

PDB references: 1j1c and 1j1b

Online 25 February 2004

 

Acta Cryst. (2004). D60, 447-452  [ doi:10.1107/S0907444903029482 ]

Structure determination of a truncated dimeric splicing endonuclease in pseudo-face-centered space group P2₁2₁2

Y. Zhang and H. Li

Synopsis: The molecular replacement solution of a dimeric splicing endonuclease in pseudo-face-centered space group P2₁2₁2 is reported.

PDB reference: 1r0v

Online 25 February 2004

 

Acta Cryst. (2004). D60, 453-462  [ doi:10.1107/S0907444903029718 ]

Complexed and ligand-free high-resolution structures of urate oxidase (Uox) from Aspergillus flavus: a reassignment of the active-site binding mode

P. Retailleau, N. Colloc'h, D. Vivarès, F. Bonneté, B. Castro, M. El Hajji, J.-P. Mornon, G. Monard and T. Prangé

Synopsis: High-resolution structures of urate oxidase-inhibitor complexes lead to a reformulation of the active-site topology.

PDB references: 1r56, 1r51, 1r4s and 1r4u

Online 25 February 2004

 

Acta Cryst. (2004). D60, 463-471  [ doi:10.1107/S090744490302969X ]

Structural study of the type II 3-dehydroquinate dehydratase from Actinobacillus pleuropneumoniae

D. Maes, L. A. Gonzalez-Ramirez, J. Lopez-Jaramillo, B. Yu, H. De Bondt, I. Zegers, E. Afonina, J. M. Garcia-Ruiz and S. Gulnik

Synopsis: The structure of the type II dehydroquinate dehydratase (DHQase) from A. pleuropneumoniae, the third enzyme of the shikimate pathway, has been determined.

PDB reference: 1uqr

Online 25 February 2004

 

Acta Cryst. (2004). D60, 472-478  [ doi:10.1107/S0907444903029767 ]

Crystal packing in two pH-dependent crystal forms of rhamnogalacturonan acetylesterase

A. Mølgaard and S. Larsen

Synopsis: The glycoprotein rhamnogalacturonan acetylesterase has been crystallized in two different polymorphic modifications: an orthorhombic crystal form which appeared at pH 5.0 and a trigonal form which was formed at pH 4.5. The differences in crystal packing can be explained by the pH-dependent variation in the protonation state of glutamic acids on the protein surface.

PDB reference: 1pp4

Online 25 February 2004

 

Acta Cryst. (2004). D60, 479-485  [ doi:10.1107/S0907444904000174 ]

Enzymatic and structural characterization of non-peptide ligand-cyclophilin complexes

G. Kontopidis, P. Taylor and M. D. Walkinshaw

Synopsis: Complexes of two piperidine ligands provide the first non-peptide cyclophilin-inhibitor structures. Enzymatic solution studies show K_i values of greater than 20 mM.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 486-492  [ doi:10.1107/S0907444904000423 ]

Structure of the biotin carboxylase subunit of pyruvate carboxylase from Aquifex aeolicus at 2.2 Å resolution

S. Kondo, Y. Nakajima, S. Sugio, J. Yong-Biao, S. Sueda and H. Kondo

Synopsis: The crystal structure of the biotin carboxylase subunit of pyruvate carboxylase from A. aeolicus (PC-) was determined at 2.2 Å resolution. One PC- molecule is contained in the asymmetric unit with space group P2₁2₁2; electron density for domain B was clearly shown in the final model.

PDB reference: 1ulz

Online 25 February 2004

 

Acta Cryst. (2004). D60, 493-498  [ doi:10.1107/S090744490400037X ]

The structure and refinement of apocrustacyanin C₂ to 1.3 Å resolution and the search for differences between this protein and the homologous apoproteins A₁ and C₁

J. Habash, J. R. Helliwell, J. Raftery, M. Cianci, P. J. Rizkallah, N. E. Chayen, G. A. Nneji and P. F. Zagalsky

Synopsis: The lobster crustacyanin C₂ protein subunit structure is reported at 1.3 Å resolution. A search for chemical structure post-translational modifications was made using three B-factor plot evaluations, but none proved sensitive enough to explain the C₂ protein chromatography signature differences from the homologous A₁ or C₁ protein subunits.

PDB references: 1s2p and 1s44

Online 25 February 2004

 

Acta Cryst. (2004). D60, 499-506  [ doi:10.1107/S0907444904000617 ]

Monitoring the anomalous scattering signal and noise levels in X-ray diffraction of crystals

Z.-Q. Fu, J. P. Rose and B.-C. Wang

Synopsis: An index R_as is proposed based on analysis of the Bijvoet differences of acentric and centric reflections to better estimate the accuracy and strength of the anomalous scattering signal in data.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 507-511  [ doi:10.1107/S0907444904000034 ]

Structure of Escherichia coli YfdW, a type III CoA transferase

A. Gogos, J. Gorman and L. Shapiro

Synopsis: Crystal structures are reported for free and coenzyme A (CoA) bound forms of the YfdW protein from E. coli, a representative type III CoA transferase.

PDB references: 1pqy, 1q7e and 1q6y

Online 25 February 2004

 

Acta Cryst. (2004). D60, 512-514  [ doi:10.1107/S090744490302852X ]

Preparation and X-ray crystallographic analysis of the Ca²⁺-discharged photoprotein obelin

L. Deng, S. V. Markova, E. S. Vysotski, Z.-J. Liu, J. Lee, J. Rose and B.-C. Wang

Synopsis: Crystals of the Ca²⁺-discharged W92F mutant of obelin from Obelia longissima have been grown, representing the first crystallization of a photoprotein after the Ca²⁺-triggered bioluminescence and bound with product, coelenteramide.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 515-517  [ doi:10.1107/S0907444904000721 ]

Crystallization and preliminary X-ray crystallographic studies of the small form of glucose-inhibited division protein A from Thermus thermophilus HB8

W. Iwasaki, H. Miyatake, A. Ebihara and K. Miki

Synopsis: The small form of glucose-inhibited division protein A from T. thermophilus HB8 has been crystallized and its diffraction data have been collected to 1.65 Å resolution. Crystals belong to the trigonal space group P3₁21 or P3₂21.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 518-520  [ doi:10.1107/S0907444903028300 ]

Crystallization of Pseudomonas aeruginosa AHL synthase LasI using -turn crystal engineering

T. A. Gould, W. T. Watson, K.-H. Choi, H. P. Schweizer and M. E. A. Churchill

Synopsis: The acyl-homoserinelactone synthase LasI has been crystallized in space group F23, with unit-cell parameters a = b = c = 154.90 Å. Crystals were obtained using a -turn engineering approach.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 521-523  [ doi:10.1107/S0907444903028294 ]

Expression and crystallization of several forms of the Propionibacterium shermanii transcarboxylase 5S subunit

P. R. Hall, R. Zheng, M. Pusztai-Carey, F. van den Akker, P. R. Carey and V. C. Yee

Synopsis: The crystallizations of three different forms of the dimeric outer 5S subunit of transcarboxylase differ in their requirement for the addition of lithium sulfate.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 524-527  [ doi:10.1107/S0907444903028488 ]

Cloning, purification, crystallization and X-ray analysis of the Escherichia coli pyrimidine nucleoside hydrolase YeiK

B. Giabbai and M. Degano

Synopsis: The E. coli yeiK gene product has been overexpressed in recombinant form and characterized as a pyrimidine nucleoside hydrolase. Crystals diffracting to 1.7 Å resolution are reported.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 528-530  [ doi:10.1107/S0907444903028427 ]

Purification, crystallization and preliminary X-ray analysis of native and selenomethionine class I tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes

B. Liotard and J. Sygusch

Synopsis: Recombinant class I D-tagatose-1,6-bisphosphate aldolase from S. pyogenes was expressed in E. coli, purified and crystallized. Crystals of both native and selenomethionine isoforms were obtained and are suitable for structural analysis.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 531-533  [ doi:10.1107/S0907444903028713 ]

Crystallization and preliminary X-ray analysis of Aes, an acetyl-esterase from Escherichia coli

K. Gerber, A. Schiefner, P. Seige, K. Diederichs, W. Boos and W. Welte

Synopsis: Aes has been crystallized as an N-terminally His₆-tagged protein both in the native form and with selenomethionine substitution.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 534-536  [ doi:10.1107/S0907444903028877 ]

Expression, purification and preliminary crystallographic analysis of phosphoribosyl isomerase (PriA) from Streptomyces coelicolor

H. Wright, F. Barona-Gómez, D. A. Hodgson and V. Fülöp

Synopsis: A novel bifunctional enzyme, phosphoribosyl isomerase, involved in both the histidine- and tryptophan-biosynthesis pathways, has been crystallized. Crystals diffract to 2 Å resolution and are suitable for X-ray structure determination.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 537-538  [ doi:10.1107/S0907444903028919 ]

Crystallization and preliminary X-ray analysis of GlcNAc1,4Gal-releasing endo--galactosidase from Clostridium perfringens

L. Deng, Z.-J. Liu, H. Ashida, S.-C. Li, Y.-T. Li, P. Horanyi, W. Tempel, J. Rose and B.-C. Wang

Synopsis: The unique clostridial endo--galactosidase (Endo--GalGnGa) capable of releasing the disaccharide GlcNAc1,4Gal from O-glycans expressed in the gastric gland mucous cell-type mucin has been crystallized.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 539-541  [ doi:10.1107/S090744490302907X ]

Crystallization and phasing of focal adhesion protein 52 from Gallus gallus

I. Töro, M. Nikki, T. Glumoff, V.-P. Lehto and K. Djinovic Carugo

Synopsis: Focal adhesion protein 52 (FAP52) is a multidomain adaptor protein composed of an N-terminal -helical domain and C-terminal SH3 domain connected by a predicted unstructured region. FAP52 was crystallized in two crystal forms and initial phase information was obtained from xenon-pressurized crystals using diffraction data collected at two softer X-ray wavelengths.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 542-544  [ doi:10.1107/S0907444903029123 ]

Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5

T. Parkkinen, N. Hakulinen, M. Tenkanen, M. Siika-aho and J. Rouvinen

Synopsis: Xylanase IV from T. reesei has been crystallized and X-ray diffraction data have been collected to 2.2 Å resolution.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 545-549  [ doi:10.1107/S0907444903029305 ]

A new crystal form of XT6 enables a significant improvement of its diffraction quality and resolution

M. Bar, G. Golan, M. Nechama, G. Zolotnitsky, Y. Shoham and G. Shoham

Synopsis: The extracellular xylanase from Geobacillus stearothermophilus T-6 (XT6) has recently been crystallized in a new monoclinic crystal form. The new crystals diffract to better than 1.5 Å resolution and show significantly improved crystallographic properties as compared with the previous crystals of XT6 of the hexagonal form.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 550-552  [ doi:10.1107/S0907444903029378 ]

Crystallization and preliminary crystallographic studies of the NCoA-1/SRC-1 PAS-B domain bound to the LXXLL motif of the STAT6 transactivation domain

A. Razeto, E. Pfitzner and S. Becker

Synopsis: Crystallographic analysis of the first complex between a PAS domain and another protein.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 553-554  [ doi:10.1107/S0907444903029317 ]

Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybus

M. Verhaest, W. Van den Ende, M. Yoshida, K. Le Roy, Y. Peeraer, S. Sansen, C. J. De Ranter, A. Van Laere and A. Rabijns

Synopsis: Crystals of fructan exohydrolase IIa from C. intybus have been prepared and proved to be suitable for successful structural analysis.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 555-557  [ doi:10.1107/S0907444903029330 ]

Crystallization and preliminary X-ray diffraction study of two complexes of a TAXI-type xylanase inhibitor with glycoside hydrolase family 11 xylanases from Aspergillus niger and Bacillus subtilis

S. Sansen, C. J. De Ranter, K. Gebruers, K. Brijs, C. M. Courtin, J. A. Delcour and A. Rabijns

Synopsis: Crystals of two complexes of an endoxylanase inhibitor from T. aestivum L. with a family 11 xylanase have been prepared. The crystals of the complex TAXI I-A. niger xylanase as well as the crystals of TAXI I in complex with B. subtilis xylanase proved to be suitable for successful structural analysis.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 558-560  [ doi:10.1107/S0907444903029494 ]

Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30

K. Beis, J. Nesper, C. Whitfield and J. H. Naismith

Synopsis: A novel integral membrane lipoprotein, Wza, from E. coli serotype O9a:K30 has been purified and crystallized.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 561-563  [ doi:10.1107/S090744490302972X ]

Crystallization and preliminary crystallographic analysis of the Escherichia coli water channel AqpZ

B. V. Daniels, J.-S. Jiang and D. Fu

Synopsis: AqpZ is a 24 kDa integral membrane protein that facilitates water movement across the plasma membrane of E. coli. The first crystallization and preliminary X-ray analysis of AqpZ are described in this study.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 564-566  [ doi:10.1107/S0907444903029779 ]

Crystallization and preliminary X-ray crystallographic analysis of the C-terminal domain of ParC protein from Bacillus stearothermophilus

T.-J. Hsieh and N.-L. Chan

Synopsis: The 36 kDa C-terminal domain of ParC from B. stearothermophilus has been crystallized in the trigonal space group P3₁ (or P3₂), with unit-cell parameters a = b = 83.5, c = 45.1 Å. A complete native data set to 2.0 Å has been collected.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 567-568  [ doi:10.1107/S0907444904000265 ]

Cloning, expression, purification, crystallization and preliminary X-ray analysis of human liver glyceraldehyde-3-phosphate dehydrogenase

M. Warizaya, T. Kinoshita, A. Kato, H. Nakajima and T. Fujii

Synopsis: Human liver glyceraldehyde-3-phosphate dehydrogenase was purified and crystallized. Crystals belong to space group P2₁, with unit-cell parameters a = 63.23, b = 97.84, c = 84.23 Å, = 104.1°, and show diffraction to 2.8 Å resolution.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 569-571  [ doi:10.1107/S0907444904000058 ]

Crystallization and preliminary X-ray crystallographic analysis of macrophage/microglia-specific calcium-binding protein Iba1

M. Yamada, Y. Imai, S. Kohsaka and S. Kamitori

Synopsis: Macrophage/microglia-specific calcium-binding protein Iba1s from human and mouse were overexpressed and crystallized. A preliminary X-ray crystallographic analysis of the crystals is reported.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 572-573  [ doi:10.1107/S090744490400006X ]

Crystallization and preliminary X-ray study of isomaltodextranase from Arthrobacter globiformis

M. Akita, M. Mizuno, T. Tonozuka, Y. Sakano, H. Matsui, Y. Hidaka, Y. Hatada, S. Ito and K. Horikoshi

Synopsis: Monoclinic isomaltodextranase crystals which diffract X-rays to 1.8 Å resolution on a rotating-anode generator were grown from PEG 6000 and ammonium sulfate.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 574-576  [ doi:10.1107/S0907444904000277 ]

Expression, purification and preliminary X-ray characterization of histidinol phosphate phosphatase

R. Omi, M. Goto, N. Nakagawa, I. Miyahara and K. Hirotsu

Synopsis: A recombinant form of histidinol phosphate phosphatase from T. thermophilus HB8 has been expressed in E. coli, purified and crystallized in crystal forms I and II, belonging to space groups P2₁2₁2 and C222₁, respectively. Diffraction data were collected to 1.70 and 1.75 Å resolution for crystal forms I and II, respectively.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 577-579  [ doi:10.1107/S0907444904000630 ]

Crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3

B. Liu, X. Li, R. Gao, W. Zhou, G. Xie, M. Bartlam, H. Pang, Y. Feng and Z. Rao

Synopsis: Inorganic pyrophosphatase from the hyperthermophile P. horikoshii has been crystallized by the hanging-drop vapour-diffusion method at pH 5.0 using polyethyleneglycol 4000 as the precipitant.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 580-582  [ doi:10.1107/S090744490400099X ]

Purification, crystallization and preliminary X-ray analysis of the GTP-binding protein Rab9 implicated in endosome-to-TGN vesicle trafficking

J. G. Wittmann and M. G. Rudolph

Synopsis: Growth of hemihedrally twinned crystals of the small GTP-binding protein Rab9, which is implicated in vesicular transport from endosomes to the trans-Golgi network, in complex with GDP is described.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 583-585  [ doi:10.1107/S0907444904001088 ]

Crystallization and preliminary X-ray analysis of family 39 -D-xylosidase from Geobacillus stearothermophilus T-6

M. Czjzek, T. Bravman, B. Henrissat and Y. Shoham

Synopsis: The crystallization and preliminary X-ray analysis of the -D-xylosidase from G. stearothermophilus T-6, a family 39 glycoside hydrolase, is described. Purified native and selenomethionine-containing enzymes were crystallized and belong to space group P2₁2₁2₁. The crystal diffracted X-rays to a resolution of 2.1 Å.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 586-587  [ doi:10.1107/S0907444904001076 ]

Crystallization and preliminary X-ray study of -type cyclodextrin glycosyltransferase from Bacillus clarkii

M. Akita, Y. Hatada, Y. Hidaka, Y. Ohta, M. Takada, Y. Nakagawa, K. Ogawa, T. Nakakuki, S. Ito and K. Horikoshi

Synopsis: A -type cyclodextrin glycosyltransferase that produces -cyclodextrin as the main product has been crystallized and analyzed by X-ray diffraction. Phasing was successfully carried out using the molecular-replacement method.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 588-590  [ doi:10.1107/S0907444904001052 ]

Production, crystallization and preliminary X-ray crystallographic studies of the bacteriophage 12 packaging motor

E. J. Mancini, D. E. Kainov, H. Wei, P. Gottlieb, R. Tuma, D. H. Bamford, D. I. Stuart and J. M. Grimes

Synopsis: Native and selenomethionyl-labelled protein of the ssRNA-packaging ATPase from bacteriophage 12 crystallizes in space groups I222 and P1. Data have been collected for both space groups, to 2.5 and 2.0 Å resolution, respectively.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 591-593  [ doi:10.1107/S0907444904001167 ]

Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1

N. Kulahin, C. Kasper, M. Gajhede, V. Berezin, E. Bock and J. S. Kastrup

Synopsis: NCAM Ig1-4, NCAM Ig1-5 and the whole extracellular part of L1 were crystallized. Preliminary data have been collected on NCAM Ig1-4 crystals belonging to space group P622 or P6₃22.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 594-596  [ doi:10.1107/S0907444903029676 ]

Rapid screening for HIV-1 protease inhibitor leads through X-ray diffraction

B. Pillai, K. K. Kannan, S. V. Bhat and M. V. Hosur

Synopsis: Hexagonal crystals of unliganded tethered HIV-1 protease combined with the soaking method for the preparation of crystalline complexes have been shown to be suitable for the rapid discovery of protease inhibitors as drugs against AIDS.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 597-600  [ doi:10.1107/S0907444903029202 ]

LySDB - Lysozyme Structural DataBase

K. Samaya Mohan, S. Das, C. Chockalingam, V. Shanthi and K. Sekar

Synopsis: LySDB is a centralized web resource with a user-friendly search engine developed to study the properties of the lysozyme structures available in the literature.

Online 25 February 2004

    

Acta Cryst. (2004). D60, 601-605  [ doi:10.1107/S0907444903029664 ]

Microseed matrix screening to improve crystals of yeast cytosine deaminase

G. C. Ireton and B. L. Stoddard

Synopsis: A crystallization strategy termed `microseed matrix screening' is described where the optimal conditions for nucleation versus extended lattice growth are not compatible.

PDB reference: 1rb7

Online 25 February 2004

 

Acta Cryst. (2004). D60, 606-609  [ doi:10.1107/S0907444903029585 ]

Production and preliminary analysis of perdeuterated yeast inorganic pyrophosphatase crystals suitable for neutron diffraction

V. U. Tuominen, D. A. A. Myles, M.-T. Dauvergne, R. Lahti, P. Heikinheimo and A. Goldman

Synopsis: Perdeuterated yeast inorganic pyrophosphatase has been produced and crystallized. Neutron diffraction data have been recorded and the structure has been determined by X-ray diffraction.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 610-612  [ doi:10.1107/S0907444903029640 ]

Novel buffer systems for macromolecular crystallization

J. Newman

Synopsis: Broad-range buffer systems that consist of three component buffers can be substituted for a single buffer in crystallization trials, allowing pH and buffer type to be decoupled.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 613-615  [ doi:10.1107/S0907444904000861 ]

Soybean lipoxygenase-3 in complex with 4-nitrocatechol

E. Skrzypczak-Jankun, O. Y. Borbulevych and J. Jankun

Synopsis: The complex of lipoxygenase-3 with 4-nitrocatechol indicates His518 withdrawal from the iron coordination sphere and flexibility of the geometrical features of this site.

PDB reference: 1no3

Online 25 February 2004

 

Acta Cryst. (2004). D60, 616  [ doi:10.1107/S0907444904002616 ]

Comment on Flash-cooling of macromolecular crystals to overcome increased mosaicity by Yao, Yasutake & Tanaka (2004)

D. F. Sargent and T. J. Richmond

Synopsis: Comment on Yao et al. (2004), Acta Cryst. D60, 39-45.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 617  [ doi:10.1107/S0907444904001271 ]

Structural studies of Proteus mirabilis catalase in its ground state, oxidized state and in complex with formic acid. Erratum

P. Andreoletti, A. Pernoud, G. Sainz, P. Gouet and H. M. Jouve

Synopsis: An erratum to the paper by Andreoletti et al. (2003), Acta Cryst. D59, 2163-2168.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 618  [ doi:10.1107/S0907444904002598 ]

Solving the structure of the bubble protein using the anomalous sulfur signal from single-crystal in-house Cu K diffraction data only. Erratum

J. G. Olsen, C. Flensburg, O. Olsen, M. Seibold, G. Bricogne and A. Henriksen

Synopsis: An erratum to the paper by Olsen et al. (2004), Acta Cryst. D60, 250-255.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 619  [ doi:10.1107/S0907444904002604 ]

The high-mosaicity illusion: revealing the true physical characteristics of macromolecular crystals. Erratum

H. D. Bellamy, E. H. Snell, J. Lovelace, M. Pokross and G. E. O. Borgstahl

Synopsis: A second erratum to the paper by Bellamy et al. Acta Cryst. (2000), D56, 986-995.

Online 25 February 2004

 

Acta Cryst. (2004). D60, 620  [ doi:10.1107/S0907444904002951 ]

Structure of the saccharide-binding domain of the human natural killer cell inhibitory receptor p75/AIRM1. Erratum

N. Dimasi, H. Attrill, D. M. F. van Aalten, A. Moretta, L. Moretta, R. Biassoni and R. A. Mariuzza

Synopsis: An erratum to the paper by Dimasi et al. (2004), Acta Cryst. D60, 401-403.

Online 25 February 2004