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Figure 3 The three images produced by the microscope: transmitted intensity (I) (top), orientation () (centre) and sin (bottom). (a), (c) and (e) show a room-temperature HEWL crystal. The variation in orientation seen in the background of the central image arises from reflection at the surface of the glass capillary within which the crystal was mounted. (b), (d) and (f) show a cryocooled (100 K) glucose isomerase crystal. Note that the orientation (c and d) stays constant across the face of the crystals, while sin (e and f) varies as the thickness of the crystal changes.

© International Union of Crystallography 2005