Acta Crystallographica Section D

Biological Crystallography

Volume 61, Part 5 (May 2005)

research papers

Acta Cryst. (2005). D61, 605-612 [doi:10.1107/S0907444905005809]

Cryogenic structure of the photosynthetic reaction center of Blastochloris viridis in the light and dark

R. H. G. Baxter, B.-L. Seagle, N. Ponomarenko and J. R. Norris

Abstract: The structure of the Blastochloris viridis photosynthetic reaction center has been determined at 100 K by flash-freezing crystals. A data set to 2.2 Å resolution provides a well determined model of the wild-type protein. Of particular interest are the position, occupancy and heterogeneity of the Q_B-binding site. Data were also collected from a crystal frozen immediately after illumination. The data support predominant binding of Q_B in the proximal position in both the neutral and charge-separated states.

PDB reference: 1vrn

Keywords: bacterial photosynthesis; cryogenic freeze-trapping.


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