Acta Crystallographica Section D

Biological Crystallography

Volume 62, Part 4 (April 2006)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: A small molecule inhibitor at the active site of E. coli methionine aminopeptidase. This inhibitor shows remarkable selectivity towards the MnII-form of the enzyme (p. 425). MnII ions are the two partially exposed green spheres.

research papers


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Acta Cryst. (2006). D62, 349-355  [ doi:10.1107/S0907444906000825 ]

The structure of the hexagonal crystal form of hen egg-white lysozyme

C. Brinkmann, M. S. Weiss and E. Weckert

Synopsis: Hen egg-white lysozyme has been crystallized in a hexagonal crystal form and the structure of this crystal form has been determined to 1.46 Å resolution.

PDB reference: 2fbb

Online 18 March 2006


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Acta Cryst. (2006). D62, 356-367  [ doi:10.1107/S0907444906000862 ]

Hydrophobin HFBII in detail: ultrahigh-resolution structure at 0.75 Å

J. Hakanpää, M. Linder, A. Popov, A. Schmidt and J. Rouvinen

Synopsis: The structure of T. reesei hydrophobin HFBII is described to an ultrahigh resolution of 0.75 Å. The structure has been carefully analyzed and comparison is made to a previously published atomic resolution structure of the same protein as well as to other ultrahigh-resolution protein structures found in the PDB.

PDB reference: 2b97

Online 18 March 2006


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Acta Cryst. (2006). D62, 368-374  [ doi:10.1107/S0907444906001399 ]

Absorption spectroscopy of three-dimensional bacteriorhodopsin crystals at cryogenic temperatures: effects of altered hydration

E. Portuondo-Campa, S. Schenkl, M. Dolder, M. Chergui, E. M. Landau and S. Haacke

Synopsis: When a strict sampling handling protocol is respected, low-temperature absorption spectra of three-dimensional bR crystals, grown in lipidic cubic phase, are almost identical to those obtained for purple membrane. Slight changes of the crystal hydration lead to drastic and irreversible modifications of the spectrum that are to be avoided in the context of high-resolution crystallography.

Online 18 March 2006


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Acta Cryst. (2006). D62, 375-382  [ doi:10.1107/S0907444906001314 ]

Structural phase transition of monoclinic crystals of hen egg-white lysozyme

K. Harata and T. Akiba

Synopsis: The crystal-to-crystal phase transition of monoclinic hen egg-white lysozyme mediated by dehydration proceeds by way of an intermediate state containing two types of microcrystals: original and transformed. The enhancement of the diffraction power of the transformed crystal is mostly ascribable to the suppression of rigid-body motion in the densely packed crystal.

PDB references: 2d4i, 2d4j and 2d4k

Online 18 March 2006


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Acta Cryst. (2006). D62, 383-391  [ doi:10.1107/S0907444906001600 ]

Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal

R. Agarwal, J. B. Bonanno, S. K. Burley and S. Swaminathan

Synopsis: The crystal structure of an FMN reductase has been determined by sulfur-SAD. The structure of the complex with the cofactor FMN is also presented.

PDB references: 1rtt and 1x77

Online 18 March 2006


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Acta Cryst. (2006). D62, 392-397  [ doi:10.1107/S0907444906001612 ]

Third calcium ion found in an inhibitor-bound phospholipase A2

K. Sekar, D. Gayathri, D. Velmurugan, J. Jeyakanthan, T. Yamane, M.-J. Poi and M.-D. Tsai

Synopsis: The paper reports the crystal structure of triple mutant (K53,56,121M) phospholipase A2 complexed with the inhibitor anisic acid.

PDB reference: 2b96

Online 18 March 2006


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Acta Cryst. (2006). D62, 398-409  [ doi:10.1107/S0907444906002204 ]

A method for finding candidate conformations for molecular replacement using relative rotation between domains of a known structure

J. I. Jeong, E. E. Lattman and G. S. Chirikjian

Synopsis: A method has been developed to obtain candidate conformations of multidomain proteins for use in molecular replacement. After obtaining the relative rotation between domains, iterative normal-mode analysis was used to drive the template structure near to the target conformation.

Online 18 March 2006


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Acta Cryst. (2006). D62, 410-416  [ doi:10.1107/S0907444906003660 ]

Specific binding of non-steroidal anti-inflammatory drugs (NSAIDs) to phospholipase A2: structure of the complex formed between phospholipase A2 and diclofenac at 2.7 Å resolution

N. Singh, T. Jabeen, S. Sharma, R. K. Somvanshi, S. Dey, A. Srinivasan and T. P. Singh

Synopsis: The structure of the complex formed between phospholipase A2 (PLA2) and diclofenac revealed the substrate-binding site of PLA2 to be physically blocked by diclofenac with a strong affinity, making several interactions with the enzyme. Surface plasmon studies indicated a value of 4.8 × 10-8 M for the dissociation constant (Kd).

PDB reference: 2b17

Online 18 March 2006


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Acta Cryst. (2006). D62, 417-424  [ doi:10.1107/S0907444906003763 ]

Serendipitous SAD phasing of an echinomycin-(ACGTACGT)2 bisintercalation complex

J. A. Cuesta-Seijo, M. S. Weiss and G. M. Sheldrick

Synopsis: An echinomycin-DNA complex was solved by SAD phasing with the help of an unexpected cation; the structure provides the first crystallographic evidence for the presence of both Hoogsteen and Watson-Crick base pairs flanking the chromophores of the bisintercalating antibiotic.

PDB reference: 2adw

Online 18 March 2006


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Acta Cryst. (2006). D62, 425-432  [ doi:10.1107/S0907444906003878 ]

Structural analysis of metalloform-selective inhibition of methionine aminopeptidase

S.-X. Xie, W.-J. Huang, Z.-Q. Ma, M. Huang, R. P. Hanzlik and Q.-Z. Ye

Synopsis: Three crystal structures of E. coli methionine aminopeptidase, two in the MnII form and one in the CoII form, complexed with metalloform-selective inhibitors provide the structural basis for their metalloform-selective inhibition.

PDB references: 2evm, 2evc and 2evo

Online 18 March 2006


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Acta Cryst. (2006). D62, 433-438  [ doi:10.1107/S0907444906004021 ]

Solving the structure of Escherichia coli elongation factor Tu using a twinned data set

S. E. Heffron, R. Moeller and F. Jurnak

Synopsis: A new packing motif of elongation factor Tu was determined from a twinned and mosaic crystal.

PDB reference: 2fx3

Online 18 March 2006


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Acta Cryst. (2006). D62, 439-450  [ doi:10.1107/S0907444906005270 ]

Optimal description of a protein structure in terms of multiple groups undergoing TLS motion

J. Painter and E. A. Merritt

Synopsis: The TLSMD algorithm finds an optimal multi-group TLS (translation/libration/screw) description of a protein as seen in a single crystal structure. This description is an approximation to intrinsic dynamic properties of the protein such as interdomain motion and local flexibility. TLSMD-generated models can improve crystallographic refinement and can offer insight into biologically relevant dynamic properties of the protein.

Online 18 March 2006


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Acta Cryst. (2006). D62, 451-457  [ doi:10.1107/S0907444906005233 ]

Rapid and simple protein-stability screens: application to membrane proteins

A. P. Yeh, A. McMillan and M. H. B. Stowell

Synopsis: A simple and rapid high-throughput screen for determining relative stabilities of proteins has been developed and specific application to membrane proteins is demonstrated.

Online 18 March 2006


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Acta Cryst. (2006). D62, 458-464  [ doi:10.1107/S0907444906005221 ]

Structure of human TSG101 UEV domain

A. Palencia, J. C. Martinez, P. L. Mateo, I. Luque and A. Camara-Artigas

Synopsis: The X-ray crystal structure of human TSG101 UEV domain has been determined at 2.2 Å resolution and refined to an R factor of 18.8%.

PDB reference: 2f0r

Online 18 March 2006


book reviews


 

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Acta Cryst. (2006). D62, 465-466  [ doi:10.1107/S0907444906008729 ]

Biofunctionalization of Nanomaterials

Online 18 March 2006


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