May 2006 issue
X-ray and EM data, together with normal-mode analysis of molecular templates, are used to obtain a molecular-replacement solution for crystal structures containing multimeric flexible molecules.
The crystal structure of T4moC from the toluene 4-monooxygenase of P. mendocina KR1 has been determined at a resolution of 1.48 Å. Structural analysis and comparison of T4moC with other structurally characterized Rieske-type ferredoxins is reported.
The first X-ray structure of the catalytic nucleotide-binding subunit A of the A1-ATPase has been determined at 2.55 Å resolution.
Structures of HIV-1 protease in complex with pseudopeptide inhibitors give new insights into the influence of the R/S configuration of the isostere and its hydroxy group on the efficiency of hydroxyethylamine and ethylenamine inhibitors.
A vesicular stomatitis virus protein oligomer complexed with RNA has been crystallized and the diffraction limits of the crystals improved by a simple soaking procedure. The internal symmetry of the oligomer and size constraints were used to determine the crystal packing arrangement.
Despentapeptide (des-B26-B30) insulin (DPI), an active modified insulin, has been crystallized in the presence of 20% acetic acid pH 2. A crystal structure analysis to 1.8 Å spacing (space group I222) revealed that the DPI molecule, which is unable to make β-strand interactions for physiological dimer formation and is apparently monomeric in solution, formed an alternative lattice-generated dimer.
Crystal structures of 2,4-dinitrophenol bound to wild-type TTR and to two amyloidogenic variants, TTR L55P and TTR Y78F, have been determined. The structural features responsible for the interaction between 2,4-dinitrophenol and transthyretin and its stabilizing effect on the protein is discussed.
The adaptability of two novel oil- and water-based cryoprotection reagents has been examined for the cryoprotection of crystals of five different proteins. Use of these versatile cryoprotectants in the presence of heavy-atom reagents allows rapid and efficient preparation of heavy-atom derivative crystals.
The crystal structure of the red fluorescent protein zRFP574 from Zoanthus sp. at 2.4 Å resolution has revealed a novel chromophore with a decarboxylated side chain of the chromophore-forming Asp66 residue.
The connectivity-based ab initio phasing method was applied to obtain the first low-resolution crystallographic images from crystals of the lectin SML-2.
The structure of alcohol dehydrogenase from E. histolytica has been determined at 1.8 Å.
The crystal structures of three mutants of L. casei folylpolyglutamate synthetase have been determined, along with the structure of the apoenzyme. The mutation of Gly51 to a serine at the ATP-binding site results in a major conformational change in an adjacent loop (the Ω-loop, residues 72–82). This structural rearrangement results in an inactive enzyme and points to the critical role played by the Ω-loop in both magnesium and folate substrate binding.
A new system is described for rapidly screening the effect of temperature on protein crystallization.
The inactive variant C60S of Mycobacterium tuberculosis thiol peroxidase shows the reduced form of the atypical 2-Cys peroxiredoxin.