Biological Crystallography

 

Acta Cryst. (2006). D62, 467-475  [ doi:10.1107/S0907444906005361 ]

Combining experimental data for structure determination of flexible multimeric macromolecules by molecular replacement

S. Trapani, C. Abergel, I. Gutsche, C. Horcajada, I. Fita and J. Navaza

Synopsis: X-ray and EM data, together with normal-mode analysis of molecular templates, are used to obtain a molecular-replacement solution for crystal structures containing multimeric flexible molecules.

Online 19 April 2006

 

Acta Cryst. (2006). D62, 476-482  [ doi:10.1107/S0907444906006056 ]

Structure of T4moC, the Rieske-type ferredoxin component of toluene 4-monooxygenase

L. A. Moe, C. A. Bingman, G. E. Wesenberg, G. N. Phillips and B. G. Fox

Synopsis: The crystal structure of T4moC from the toluene 4-monooxygenase of P. mendocina KR1 has been determined at a resolution of 1.48  Å. Structural analysis and comparison of T4moC with other structurally characterized Rieske-type ferredoxins is reported.

PDB reference: 1vm9

Online 19 April 2006

 

Acta Cryst. (2006). D62, 483-488  [ doi:10.1107/S0907444906006329 ]

Structure of the catalytic nucleotide-binding subunit A of A-type ATP synthase from Pyrococcus horikoshii reveals a novel domain related to the peripheral stalk

Y. Maegawa, H. Morita, D. Iyaguchi, M. Yao, N. Watanabe and I. Tanaka

Synopsis: The first X-ray structure of the catalytic nucleotide-binding subunit A of the A₁-ATPase has been determined at 2.55  Å resolution.

PDB reference: 1vdz

Online 19 April 2006

 

Acta Cryst. (2006). D62, 489-497  [ doi:10.1107/S0907444906006718 ]

On the role of the R configuration of the reaction-intermediate isostere in HIV-1 protease-inhibitor binding: X-ray structure at 2.0  Å resolution

J. Dusková, J. Dohnálek, T. Skálová, H. Petroková, E. Vondrácková, M. Hradílek, J. Konvalinka, M. Soucek, J. Brynda, M. Fábry, J. Sedlácek and J. Hasek

Synopsis: Structures of HIV-1 protease in complex with pseudopeptide inhibitors give new insights into the influence of the R/S configuration of the isostere and its hydroxy group on the efficiency of hydroxyethylamine and ethylenamine inhibitors.

PDB references: 1zsf and 1zsr

Online 19 April 2006

 

Acta Cryst. (2006). D62, 498-504  [ doi:10.1107/S0907444906006809 ]

Resolution improvement of X-ray diffraction data of crystals of a vesicular stomatitis virus nucleocapsid protein oligomer complexed with RNA

T. J. Green and M. Luo

Synopsis: A vesicular stomatitis virus protein oligomer complexed with RNA has been crystallized and the diffraction limits of the crystals improved by a simple soaking procedure. The internal symmetry of the oligomer and size constraints were used to determine the crystal packing arrangement.

Online 19 April 2006

 

Acta Cryst. (2006). D62, 505-511  [ doi:10.1107/S0907444906006871 ]

I222 crystal form of despentapeptide (B26-B30) insulin provides new insights into the properties of monomeric insulin

J. L. Whittingham, Z. Youshang, L. Záková, E. J. Dodson, J. P. Turkenburg, J. Brange and G. G. Dodson

Synopsis: Despentapeptide (des-B26-B30) insulin (DPI), an active modified insulin, has been crystallized in the presence of 20% acetic acid pH 2. A crystal structure analysis to 1.8  Å spacing (space group I222) revealed that the DPI molecule, which is unable to make -strand interactions for physiological dimer formation and is apparently monomeric in solution, formed an alternative lattice-generated dimer.

PDB reference: 2ceu

Online 19 April 2006

 

Acta Cryst. (2006). D62, 512-519  [ doi:10.1107/S0907444906006962 ]

The binding of 2,4-dinitrophenol to wild-type and amyloidogenic transthyretin

E. Morais-de-Sá, R. M. Neto-Silva, P. J. B. Pereira, M. J. Saraiva and A. M. Damas

Synopsis: Crystal structures of 2,4-dinitrophenol bound to wild-type TTR and to two amyloidogenic variants, TTR L55P and TTR Y78F, have been determined. The structural features responsible for the interaction between 2,4-dinitrophenol and transthyretin and its stabilizing effect on the protein is discussed.

PDB references: 2b14, 2b15 and 2b16

Online 19 April 2006

 

Acta Cryst. (2006). D62, 520-526  [ doi:10.1107/S0907444906009012 ]

Novel versatile cryoprotectants for heavy-atom derivatization of protein crystals

M. Sugahara and N. Kunishima

Synopsis: The adaptability of two novel oil- and water-based cryoprotection reagents has been examined for the cryoprotection of crystals of five different proteins. Use of these versatile cryoprotectants in the presence of heavy-atom reagents allows rapid and efficient preparation of heavy-atom derivative crystals.

Online 19 April 2006

 

Acta Cryst. (2006). D62, 527-532  [ doi:10.1107/S0907444906007852 ]

Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore

N. Pletneva, S. Pletnev, T. Tikhonova, V. Popov, V. Martynov and V. Pletnev

Synopsis: The crystal structure of the red fluorescent protein zRFP574 from Zoanthus sp. at 2.4  Å resolution has revealed a novel chromophore with a decarboxylated side chain of the chromophore-forming Asp66 residue.

PDB reference: 2fl1

Online 19 April 2006

 

Acta Cryst. (2006). D62, 533-540  [ doi:10.1107/S0907444906007591 ]

Low-resolution ab initio phasing of Sarcocystis muris lectin SML-2

J. J. Müller, N. L. Lunina, A. Urzhumtsev, E. Weckert, U. Heinemann and V. Y. Lunin

Synopsis: The connectivity-based ab initio phasing method was applied to obtain the first low-resolution crystallographic images from crystals of the lectin SML-2.

Online 19 April 2006

 

Acta Cryst. (2006). D62, 541-547  [ doi:10.1107/S0907444906009292 ]

Structure of alcohol dehydrogenase from Entamoeba histolytica

L. J. W. Shimon, E. Goihberg, M. Peretz, Y. Burstein and F. Frolow

Synopsis: The structure of alcohol dehydrogenase from E. histolytica has been determined at 1.8  Å.

PDB reference: 1y9a

Online 19 April 2006

 

Acta Cryst. (2006). D62, 548-558  [ doi:10.1107/S0907444906009796 ]

Mutation of Gly51 to serine in the P-loop of Lactobacillus casei folylpolyglutamate synthetase abolishes activity by altering the conformation of two adjacent loops

C. A. Smith, J. A. Cross, A. L. Bognar and X. Sun

Synopsis: The crystal structures of three mutants of L. casei folylpolyglutamate synthetase have been determined, along with the structure of the apoenzyme. The mutation of Gly51 to a serine at the ATP-binding site results in a major conformational change in an adjacent loop (the -loop, residues 72-82). This structural rearrangement results in an inactive enzyme and points to the critical role played by the -loop in both magnesium and folate substrate binding.

PDB references: 2gca, 2gc5, 2gcb and 2gc6

Online 19 April 2006

 

Acta Cryst. (2006). D62, 559-562  [ doi:10.1107/S0907444906006093 ]

A method for screening the temperature dependence of three-dimensional crystal formation

M. J. Landsberg, J. Bond, C. L. Gee, J. L. Martin and B. Hankamer

Synopsis: A new system is described for rapidly screening the effect of temperature on protein crystallization.

Online 19 April 2006

 

Acta Cryst. (2006). D62, 563-567  [ doi:10.1107/S0907444906008249 ]

Structure of the inactive variant C60S of Mycobacterium tuberculosis thiol peroxidase

M. Stehr, H. J. Hecht, T. Jäger, L. Flohé and M. Singh

Synopsis: The inactive variant C60S of Mycobacterium tuberculosis thiol peroxidase shows the reduced form of the atypical 2-Cys peroxiredoxin.

PDB reference: 1y25

Online 19 April 2006