Structure of a calcium-deficient form of influenza virus neuraminidase: implications for substrate binding

A crystal form of influenza virus neuraminidase isolated from whale is reported in which calcium ions are absent, leading to structural heterogeneity of loops near the enzyme active site. Sulfate ions are observed in and near the active site.

Structure of a bovine secretory signalling glycoprotein (SPC-40) at 2.1 Å resolution

The crystal structure of a bovine secretory signalling glycoprotein (SPC-40) has been determined at 2.1 Å resolution. The structure reveals new features of the sugar-binding groove and the presence of a novel flexible region implicated as being involved in receptor recognition.

Structure of an ETHE1-like protein from Arabidopsis thaliana

The crystal structure of AtETHE1 has been solved to a resolution of 1.48 Å. The structure has been analyzed and comparison is made to previously published structures of glyoxalase II enzymes.

Structure of the N-terminal domain of human CEACAM1: binding target of the opacity proteins during invasion of Neisseria meningitidis and N. gonorrhoeae

The crystal structure of the N-terminal domain of human CEACAM1 is presented at 2.2 Å resolution. This structure is compared with its homologue from mouse and the binding surfaces are mapped for Neisseria Opa proteins and for other CEACAM molecules.

Structure of conkunitzin-S1, a neurotoxin and Kunitz-fold di­sulfide variant from cone snail

Most Kunitz proteins like BPTI and α-dendrotoxin are stabilized by three disulfide bonds. The crystal structure shows how subtle repacking of non-covalent interactions may compensate for disulfide bond loss in a naturally occurring two-disulfide variant, conkunitzin-S1, the first discovered member of a new conotoxin family.

Reproducible improvements in order and diffraction limit of crystals of bovine mitochondrial F₁-ATPase by controlled dehydration

The diffraction properties of crystals of bovine F₁-ATPase have been improved reproducibly by controlled dehydration, resulting in diffraction to 1.8 Å resolution.

An ensemble of crystallographic models enables the description of novel bromate-oxoanion species trapped within a protein crystal

The structure of a protein soaked in a bromate solution has been determined to a resolution of 1.25 Å.

The Buccaneer software for automated model building. 1. Tracing protein chains

A new technique for the automated tracing of protein chains in experimental electron-density maps is described. The technique relies on the application of an electron-density likelihood target function to identify likely candidate C^α positions and to then grow these into connected protein chains.

Identifying non-crystallographic symmetry in protein electron-density maps: a feature-based approach

A robust, accurate and efficient feature-based algorithm to determine NCS operators in the initial stages of the structure determination process.

Structure of plastocyanin from the cyanobacterium Anabaena variabilis

The crystal structure of the unique basic plastocyanin from A. variabilis was determined at 1.6 Å resolution.

Qu­antifying X-ray radiation damage in protein crystals at cryogenic temperatures

An experimental protocol is defined that provides a robust and reliable assessment of the radiation-sensitivity of protein crystals. Measurements on lysozyme crystals with varying heavy-ion content and on crystals of three other proteins suggest that radiation damage per dose is roughly constant at cryogenic temperatures.

Automated streak-seeding with micromachined silicon tools

An automated robotic system for protein streak-seeding is described which uses novel silicon microtools in place of the commonly used boar bristles.

The cofactor-induced pre-active conformation in PhoB

Mg²⁺ induces a flexible state in helix α4 at the PhoB receiver domain α4-β5-α5 surface.

Development of an automated large-scale protein-crystallization and monitoring system for high-throughput protein-structure analyses

An automated large-scale protein-crystallization and monitoring system for high-throughput protein structural analyses has been developed. Many protein crystals have been obtained using the system.

Integrated state evaluation for the images of crystallization droplets utilizing linear and nonlinear classifiers

The method presented here evaluates protein crystallization states using classifiers.

The 1.23 Å structure of Pichia pastoris lysyl oxidase reveals a lysine–lysine cross-link

The high-resolution structure of P. pastoris lysyl oxidase reveals a lysine–lysine cross-link presumed to result from autocatalytic oxidation of a lysine residue of the enzyme.

Structure of Methanocaldococcus jannaschii nucleoside kinase: an archaeal member of the ribokinase family

The crystal structures of the apo form and a complex of M. jannaschii nucleoside kinase have been solved at 1.7 and 1.9 Å resolution, respectively. Several structural features typical for proteins from hyperthermophilic organisms, such as increased charged and a decreased hydrophobic accessible surface area and a higher fraction of charged residues, ionic networks and large aromatic clusters, were identified.

NORMA: a tool for flexible fitting of high-resolution protein structures into low-resolution electron-microscopy-derived density maps

This paper describes a freely available software suite that allows the modelling of large conformational changes of protein structures for the interpretation of electron-microscopy reconstructions.

Structural Aspects of Protein Synthesis

Applications of single-wavelength anomalous dispersion at high and atomic resolution. Corrigendum

A corrigendum to the paper by Brodersen et al. (2000), Acta Cryst. D56, 431–441.