issue contents

Journal logoBIOLOGICAL
ISSN: 1399-0047

March 2007 issue

Highlighted illustration

Cover illustration: A sulfanilic acid molecule creates a lattice interface in a crystal of porcine trypsin (p. 310).


Acta Cryst. (2007). D63, 275
doi: 10.1107/S0907444907005185

research papers

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The crystal structure of the human p53 core domain in the absence of DNA has been determined with a new monoclinic space group (P21). Detailed analysis of non-crystallographic symmetry and crystal packing reveals that the p53 molecules are arranged differently from those in other p53 crystals, suggesting the possible existence of different p53 core domain tetramers.

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The structure of a CooA variant from the thermophilic bacterium Carboxydothermus hydrogenoformans (ChCooA) is reported in which one monomer is fully in the on-state.

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The structures of S. cerevisae glutaredoxin 1 and of a fusion protein consisting of yellow fluorescent protein and glutaredoxin were solved by molecular replacement and refined to 2.0 and 2.7 Å, respectively.

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Crystal structures with and without His-tags have been surveyed and the authors show that His-tags are not hazardous to your structure.

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A small but significant reduction of X-ray-induced radiation damage at 15 K compared with 90 K could be found in the systematic study of a total of 54 crystals of insulin and holoferritin.

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Difference Fourier X-ray diffraction analyses of nine crystals are presented, which convincingly demonstrate the validity of the hypothesis that protein crystallization can be driven by the inclusion of small molecules rich in hydrogen-bonding, hydrophobic and electrostatic bonding possibilities.

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Structure of double-stranded right-handed double-helical gramicidin channels (dimers) in the crystal of gramicidin D with KI complex has been determined. High-resolution data enabled quantitative determination of gramicidin components and distributions of the I anions outside and K cations inside the channel, which was confirmed by their anomalous scattering.

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A methodology and an instrument for the temperature-controlled optimization of crystal growth are described. The technique finds application in the growth of large high-quality crystals for neutron crystallography.

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The SAD technique was used to determine the crystal structure of a putative redox-enzyme maturation protein from A. fulgidus at 3.4 Å resolution.

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The crystal structure of glyoxylate reductase from the hyperthermophilic archaeon P. horikoshii OT3 complexed with NADP(H) was solved at 1.7 Å resolution. The present study revealed its cofactor-recognition and thermostability mechanisms.

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The structure of a transition-state analog of muscle creatine kinase reveals significant asymmetry within the functional homodimer. The amino-terminal region is shown to be intimately involved in subunit association and intersubunit communication.

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The crystal structure of the AlaX-M trans-editing enzyme revealed that the conserved glycine-rich loop in the N-terminal domain is located near the catalytic site in the C-terminal domain.

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Gramicidin S, a membrane-active antibiotic, was crystallized from solvent containing water, methanol, trifluoroacetic acid and hydrochloric acid. The structure was refined at 0.95 Å resolution and contains 1.5 molecules of gramicidin S, two trifluoracetic acid molecules and ten water molecules in the crystallographic asymmetric unit. In the crystals, the gramicidin S molecules line up into helical channels that differ from those observed previously.

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The crystal structures of two mutants of the N-terminal half-molecule of human transferrin, K206E and K206E/K296E, have identified important features of the mechanism of iron release.

short communications

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Structures of human galectin-3 carbohydrate-recognition domain determined at various stages of ligand removal provided insight into ligand-diffusion rates and their significance with respect to ligand-exchange protocols.

books received

Acta Cryst. (2007). D63, 420
doi: 10.1107/S0907444906056113

letters to the editor

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