Structures of human deoxycytidine kinase product complexes

The first crystal structures of human dCK in complex with products are reported.

Structure-assisted discovery of an amino­thiazole derivative as a lead molecule for inhibition of bacterial fatty-acid synthesis

β-Ketoacyl-ACP synthase is a key target for the treatment of infectious diseases. A structure-based biophysical screening approach identified for the first time a synthetic small molecule, 2-phenylamino-4-methyl-5-acetylthiazole, that binds to the active site of the enzyme. Implications for the use of this information in drug discovery are discussed.

Structure of the branched-chain keto acid de­carboxylase (KdcA) from Lactococcus lactis provides insights into the structural basis for the chemoselective and enantioselective carboligation reaction

Crystal structures of recombinant branched-chain keto acid decarboxylase (KdcA) from L. lactis were determined as a binary complex with the cofactor thiamin diphosphate and as a complex with an analogue of a reaction intermediate to 1.6 and 1.8 Å resolution, respectively.

Structure of the human Tim44 C-terminal domain in complex with penta­ethylene glycol: ligand-bound form

Familial oncocytic thyroid carcinoma is associated with a missense mutation, P308Q, in the C-terminal domain of Tim44. Here, the crystal structure of the human Tim44 C-terminal domain in complex with pentaethylene glycol at 1.9 Å resolution is reported.

On the interrelationship between atomic displacement parameters (ADPs) and coordinates in protein structures

A simple three-parameter ADP model for macromolecules has been devised and examined with respect to its potential use in macromolecular crystallography.

Comparison of two T-state structures of regulatory-chain mutants of Escherichia coli aspartate transcarbamoylase suggests that His20 and Asp19 modulate the response to heterotropic effectors

Two structures of regulatory chain mutants Asp19Ar and His20Ar of E. coli aspartate transcabomylase are presented. Small structural changes illustrate small, but significant, changes in kinetic behavior of both mutant enzymes and suggest that His20 and Asp19 participate in heterotropic regulatory pathways.

Triclinic lysozyme at 0.65 Å resolution

The crystal structure of triclinic hen egg-white lysozyme refined at 0.65 Å resolution is presented.

Structure of the Bacillus subtilis OhrB hydroperoxide-resistance protein in a fully oxidized state

A 2.1 Å resolution crystal structure of the B. subtilis organic hydroperoxide-resistance protein B reveals details of the stereochemistry of the disulfide bonds in the transient oxidized form of the enzyme.

Experimental determination of optimal root-mean-square deviations of macromolecular bond lengths and angles from their restrained ideal values

Comment on Jaskolski et al. [(2007), Acta Cryst. D63, 611–620].

Numerology versus reality: a voice in a recent dispute

Response to comments by Stec [(2007), Acta Cryst. D63, 1113–1114] and Tickle [(2007), Acta Cryst. D63, 1274–1281] on Jaskolski et al. [(2007), Acta Cryst. D63, 611–620].