Biological Crystallography

 

Acta Cryst. (2008). D64, 141-148  [ doi:10.1107/S090744490705559X ]

Surface-entropy reduction approaches to manipulate crystal forms of -ketoacyl acyl carrier protein synthase II from Streptococcus pneumoniae

G. Parthasarathy, R. Cummings, J. W. Becker and S. M. Soisson

Synopsis: Surface-entropy reduction (SER) mutants of the FabF protein from S. pneumoniae were rationally designed to avoid the disruption of the presumably strong interfaces involved in the formation of a high-resolution (1.3 Å) crystal form while at the same time potentially destabilizing an undesirable crystal form. One of the mutant proteins yielded a new crystal form that diffracted to high resolution, underscoring the power of the SER approach in protein and crystal engineering.

PDB reference: 2rjt

Online 16 January 2008

 

Acta Cryst. (2008). D64, 149-157  [ doi:10.1107/S0907444907057228 ]

Structures of potent selective peptide mimetics bound to carboxypeptidase B

M. Adler, B. Buckman, J. Bryant, Z. Chang, K. Chu, K. Emayan, P. Hrvatin, I. Islam, J. Morser, D. Sukovich, C. West, S. Yuan and M. Whitlow

Synopsis: A series of structures show the development of novel inhibitors of carboxypeptidase B. These inhibitors were designed to mimic peptide-based inhibitors of the protein.

PDB references: 2piy, 2piz, 2pj0, 2pj1, 2pj2, 2pj3, 2pj4, 2pj5, 2pj6, 2pj7, 2pj8, 2pj9, 2pja, 2pjb and 2pjc

Online 16 January 2008

 

Acta Cryst. (2008). D64, 158-166  [ doi:10.1107/S090744490705812X ]

Protein crystallography with a micrometre-sized synchrotron-radiation beam

R. Moukhametzianov, M. Burghammer, P. C. Edwards, S. Petitdemange, D. Popov, M. Fransen, G. McMullan, G. F. X. Schertler and C. Riekel

Synopsis: For the first time, protein microcrystallography has been performed with a focused synchrotron-radiation beam of 1 µm using a goniometer with a sub-micrometre sphere of confusion. The crystal structure of xylanase II has been determined with a flux density of about 3 × 10¹⁰ photons s^-1 µm^-2 at the sample.

PDB reference: 2jic

Online 16 January 2008

 

Acta Cryst. (2008). D64, 167-171  [ doi:10.1107/S0907444907058416 ]

A novel method for detection of selenomethionine incorporation in protein crystals via Raman microscopy

A. Vergara, A. Merlino, E. Pizzo, G. D'Alessio and L. Mazzarella

Synopsis: A novel methodological development for the analysis, via Raman microscopy, of SeMet-labelled protein crystals is presented.

Online 16 January 2008

 

Acta Cryst. (2008). D64, 172-177  [ doi:10.1107/S0907444907058659 ]

Rapid and automated substructure solution by Shake-and-Bake

H. Xu and C. M. Weeks

Synopsis: The computer program SnB, which implements the direct-methods Shake-and-Bake procedure and is part of the protein structure-determination package BnP, has recently been optimized in several ways for more rapid, automated substructure determination.

Online 16 January 2008

 

Acta Cryst. (2008). D64, 178-188  [ doi:10.1107/S0907444907059021 ]

Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIM

M. Alahuhta, M. G. Casteleijn, P. Neubauer and R. K. Wierenga

Synopsis: Crystal structures of unliganded and liganded complexes of the A178L variant of dimeric and monomeric trypanosomal TIM are reported. The A178L point mutation changes the conserved sequence pattern of the C-terminal hinge of the catalytic loop-6. The point mutation causes differences in the apo structures but not in the liganded structures.

PDB references: 2v0t, 2v2c, 2v2d and 2v2h

Online 16 January 2008

 

Acta Cryst. (2008). D64, 189-197  [ doi:10.1107/S0907444907060064 ]

In situ X-ray analysis of protein crystals in low-birefringent and X-ray transmissive plastic microchannels

J. D. Ng, P. J. Clark, R. C. Stevens and P. Kuhn

Synopsis: Plastic microchannel crystallization template designs made from inexpensive cyclic olefin copolymers have been shown to be low-birefringent, X-ray transmissive and compatible with microfluidic fabrication in restricted geometry. The model proteins thaumatin, lysozyme and bacteriorhodopsin demonstrated the feasibility of conducting counter-diffusion equilibration within the new plastic configuration.

Online 16 January 2008

 

Acta Cryst. (2008). D64, 198-205  [ doi:10.1107/S0907444907059835 ]

Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor

U. Okada, K. Kondo, T. Hayashi, N. Watanabe, M. Yao, T. Tamura and I. Tanaka

Synopsis: The crystal structure of SCO0332 was determined; functional analysis suggests that SCO0332 is a transcriptional repressor of the sco0330 gene.

PDB reference: 2zb9

Online 16 January 2008

 

Acta Cryst. (2008). D64, 206-210  [ doi:10.1107/S090744490706012X ]

Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate

R. D. Busam

Synopsis: The structure of Escherichia coli exonuclease I in complex with a nucleotide product, thymidine 5'-monophosphate, is described in an orthorhombic space group at 1.5 Å resolution.

PDB reference: 2qxf

Online 16 January 2008

 

Acta Cryst. (2008). D64, 211-218  [ doi:10.1107/S0907444907065390 ]

High-resolution structure of methionine -lyase from Citrobacter freundii

A. Nikulin, S. Revtovich, E. Morozova, N. Nevskaya, S. Nikonov, M. Garber and T. Demidkina

Synopsis: The crystal structure of methionine -lyase from Citrobacter freundii was determined at 1.35 Å. The high-resolution structure makes it possible to analyze in detail interactions between monomers, a mode of the cofactor binding and participation of the protein flexible regions in substrate recognition and binding.

PDB reference: 2fng

Online 16 January 2008

 

Acta Cryst. (2008). D64, 219-220  [ doi:10.1107/S0907444907056132 ]

Alternative models for two crystal structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate synthase

I. Le Trong and R. E. Stenkamp

Synopsis: The noncrystallographic symmetry operations reported for two crystal structures of 3,4-dihydroxy-2-butanone 4-phosphate synthase can be reinterpreted as crystallographic operations.

PDB references: 2ris and 2riu

Online 16 January 2008

 

Acta Cryst. (2008). D64, 221-226  [ doi:10.1107/S0907444907053231 ]

Notes for authors 2008

Online 16 January 2008