issue contents

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047

December 2008 issue

Highlighted illustration

Cover illustration: Boxing a sulfur SAD experiment: (top) plot of <|[Delta]Fanom|/[sigma]([Delta]anom)> versus resolution (expressed as 1/d in Å-1) at each different wavelength calculated for HEWL data sets of 360° of rotation; (center) electron-density Fourier map (1.5[sigma] cut off) covering residues 49 to 53 and calculated to 1.73 Å resolution for the X-ray data set collected at 2.070 Å wavelength with redundancy of 360 images plus 2[theta] tilt pass; (bottom) a typical lysozyme diffraction pattern; (at the side) the constrains of dose and redundancy (p. 1196).

research papers


link to html
A system for scoring images based on the likelihood of containing crystalline material is described. A simulation carried out on a real set of crystallization images demonstrates the utility of such a system in high-throughput environments by substantially reducing the manual workload necessary to detect crystals for X-ray screening.

link to html
Data collection on lysozyme as a test at six widely spaced wavelengths between 0.979 and 2.290 Å allowed determination of the sulfur substructure and produced good-quality phases, yielding information on the optimum combination of wavelength, data redundancy and X-ray dose as well as instrument geometry.

link to html
Through the combination of robust mechanized experimental hardware and a flexible control system with an intuitive user interface, SSRL researchers have screened over 200 000 biological crystals for diffraction quality in an automated fashion. Three quarters of SSRL researchers are using these data-collection tools from remote locations.

link to html
It is demonstrated that changes in the content of the mother liquor in the microseed matrix-screening method are an important contributing factor in the increased likelihood of crystallization.


link to html
Mapping crystallization results in chemical space helps to correlate seemingly distant relationships between crystallization conditions, points to possible optimization strategies and reveals promising unsampled areas of crystallization space.

link to html
A limited number of conservative mutations between two homologous antibodies does not significantly effect their structures, but does cause subtle changes in crystal packing that results in one stucture displaying pseudo-merohedral twinning while the other structure does not.

link to html
In this study, 12 mutant enzymes were created by mutating the conserved residues Gln70, Asn72, Gln81 and Glu85 proposed to bind to substrate subsites +1 and +2 and the catalytic properties of these mutants were determined.

link to html
Crystal structures of mannosyl-3-phosphoglycerate phosphatase from P. horikoshii in open and closed conformations suggest the importance of the conformational change in substrate binding and catalytic cycle regulation.

link to html
The X-ray structure of a noncovalently modified trans-3-chloroacrylic acid dehalogenase with a substrate-homolog acetate bound in the active site has been determined to 1.7 Å resolution. Elucidation of catalytically important water is reported and multiple conformations of the catalytic residue αGlu52 are observed.

link to html
The hyperthermophile S. solfataricus utilizes an unusual pathway to metabolize both glucose and galactose that can involve a 2-keto-3-deoxygluconate kinase (KDGK) that is promiscuous for KDGlu and KDGal. The crystal structure of KDGK in complex with KDGlu and an ATP analogue that suggests a structural basis for its promiscuity is presented.

short communications


link to html
Molecular replacement using ab initio derived search models produced on typical laboratory hardware with ROSETTA is successful for a number of test cases.

link to html
The 2.30 Å resolution crystal structure of CKS from H. influenza in complex with KDO has been determined. KDO bound to the active site exists as the β-pyranose form in the 5C2 chair conformation.
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds