Acta Cryst. (2010). D66, 1
[ doi:10.1107/S0907444909054481 ]

Black sheep among the flock of protein structures

Z. Dauter and E. N. Baker

Online 21 December 2009

 

Acta Cryst. (2010). D66, 2-11
[ doi:10.1107/S0907444909037731 ]

Feasibility of one-shot-per-crystal structure determination using Laue diffraction

S. Cornaby, D. M. E. Szebenyi, D.-M. Smilgies, D. J. Schuller, R. Gillilan, Q. Hao and D. H. Bilderback

Synopsis: Structure determination was successfully carried out using single Laue exposures from a group of lysozyme crystals. The Laue method may be a viable option for collection of one-shot-per-crystal data from microcrystals.

Online 21 December 2009

 

Acta Cryst. (2010). D66, 12-21
[ doi:10.1107/S0907444909042073 ]

MolProbity: all-atom structure validation for macromolecular crystallography

V. B. Chen, W. B. Arendall, J. J. Headd, D. A. Keedy, R. M. Immormino, G. J. Kapral, L. W. Murray, J. S. Richardson and D. C. Richardson

Synopsis: MolProbity structure validation will diagnose most local errors in macromolecular crystal structures and help to guide their correction.

Online 21 December 2009

 

Acta Cryst. (2010). D66, 22-25
[ doi:10.1107/S0907444909042589 ]

Molecular replacement with MOLREP

A. Vagin and A. Teplyakov

Synopsis: The molecular-replacement program MOLREP is described.

Online 21 December 2009

 

Acta Cryst. (2010). D66, 26-32
[ doi:10.1107/S0907444909042838 ]

Overcoming merohedral twinning in crystals of bacteriorhodopsin grown in lipidic mesophase

V. Borshchevskiy, R. Efremov, E. Moiseeva, G. Büldt and V. Gordeliy

Synopsis: The distribution of the twinning ratio of crystals of bacteriorhodopsin was studied under a variety of in meso crystallization conditions. A condition favouring the growth of untwinned crystals was determined.

Online 21 December 2009

 

Acta Cryst. (2010). D66, 33-43
[ doi:10.1107/S0907444909043030 ]

Structure of a family 3b' carbohydrate-binding module from the Cel9V glycoside hydrolase from Clostridium thermocellum: structural diversity and implications for carbohydrate binding

S. Petkun, S. Jindou, L. J. W. Shimon, S. Rosenheck, E. A. Bayer, R. Lamed and F. Frolow

Synopsis: The first structure of the family 3b' CBM derived from the Cel9V glycoside hydrolase of C. thermocellum, which fails to bind crystalline cellulose, is presented.

PDB references: 2wob, 2wo4 and 2wnx

Online 21 December 2009

 

Acta Cryst. (2010). D66, 44-50
[ doi:10.1107/S0907444909043972 ]

Self-interaction chromatography as a tool for optimizing conditions for membrane protein crystallization

M. Gabrielsen, L. A. Nagy, L. J. DeLucas and R. J. Cogdell

Synopsis: Monitoring changes in B values using self-interaction chromatography combined with crystallization trials resulted in single straight-edged crystals of light-harvesting complex 1-reaction centre from A. vinosum. The method allows the rapid screening of additives and detergents.

Online 21 December 2009

 

Acta Cryst. (2010). D66, 51-60
[ doi:10.1107/S0907444909044175 ]

The X-ray structure of Salmonella typhimurium uridine nucleoside phosphorylase complexed with 2,2'-anhydrouridine, phosphate and potassium ions at 1.86 Å resolution

A. A. Lashkov, N. E. Zhukhlistova, A. H. Gabdoulkhakov, A. A. Shtil, R. G. Efremov, C. Betzel and A. M. Mikhailov

Synopsis: The X-ray structure (at 1.86 Å resolution) of S. typhimurium uridine nucleoside phosphorylase complexed with its inhibitor 2,2'-anhydrouridine and phosphate and potassium ions is reported.

PDB reference: 3fwp

Online 21 December 2009

 

Acta Cryst. (2010). D66, 61-72
[ doi:10.1107/S0907444909043960 ]

The structure of dihydrodipicolinate reductase (DapB) from Mycobacterium tuberculosis in three crystal forms

R. Janowski, G. Kefala and M. S. Weiss

Synopsis: The structure of dihydrodipicolinate reductase from M. tuberculosis has been determined in three different crystal forms as the apo form of the enzyme and as a binary complex with the cofactor NADH. A comparison of the structures reveals a striking domain rotation, which can be rationalized by normal-mode analysis.

PDB references: 1yl5, 1yl6 and 1yl7

Online 21 December 2009

 

Acta Cryst. (2010). D66, 73-79
[ doi:10.1107/S0907444909045715 ]

Adenosine binding to low-molecular-weight purine nucleoside phosphorylase: the structural basis for recognition based on its complex with the enzyme from Schistosoma mansoni

H. M. Pereira, M. M. Rezende, M. S. Castilho, G. Oliva and R. C. Garratt

Synopsis: The low molecular weight PNP from Schistosoma mansoni is shown to bind adenosine in a similar fashion to that observed for the 6-oxo purine substrates. This important difference with respect to the human enzyme, which is unable to bind adenosine, is explained on the basis of minor details to the active site and the corresponding water structure.

PDB references: 3f8w, 3faz, 3fnq and 3e9r

Online 21 December 2009

 

Acta Cryst. (2010). D66, 80-87
[ doi:10.1107/S0907444909046411 ]

Structural analysis of an MK2-inhibitor complex: insight into the regulation of the secondary structure of the Gly-rich loop by TEI-I01800

A. Fujino, K. Fukushima, N. Namiki, T. Kosugi and M. Takimoto-Kamimura

Synopsis: The crystal structure of MK2 bound to TEI-I01800, a pyrazolo[1,5-a]pyrimidine-scaffold inhibitor, showed an -helical Gly-rich loop. The secondary structure of the Gly-rich loop might be regulated by the ligand structure.

PDB reference: 3a2c

Online 21 December 2009

 

Acta Cryst. (2010). D66, 88-96
[ doi:10.1107/S0907444909046642 ]

Two crystal modifications of (Pro-Pro-Gly)₄-Hyp-Hyp-Gly-(Pro-Pro-Gly)₄ reveal the puckering preference of Hyp(X) in the Hyp(X):Hyp(Y) and Hyp(X):Pro(Y) stacking pairs in collagen helices

K. Okuyama, T. Morimoto, H. Narita, T. Kawaguchi, K. Mizuno, H. P. Bächinger, G. Wu and K. Noguchi

Synopsis: Two crystal modifications of a collagen model peptide with a Hyp-Hyp-Gly sequence in the middle of 27 residues were analyzed at high resolution. Differences between the molecular interactions of these modifications revealed clear preferences for the Hyp(X) puckering.

PDB references: 3a08 and 3a19

Online 21 December 2009

 

Acta Cryst. (2010). D66, 97-109
[ doi:10.1107/S0907444909031205 ]

A toolkit for the characterization of CCD cameras for transmission electron microscopy

M. Vulovic, B. Rieger, L. J. van Vliet, A. J. Koster and R. B. G. Ravelli

Synopsis: A toolkit for the characterization of CCD cameras has been developed. Its use has been demonstrated by the characterization of three in-house transmission electron microscopy detectors, yielding statistics such as the number of outliers, the modulation transfer function, the conversion factor, the effective gain and the detective quantum efficiency.

Online 21 December 2009

 

Acta Cryst. (2010). D66, 110-114
[ doi:10.1107/S0907444909047830 ]

Notes for authors 2010

Online 21 December 2009