Biological Crystallography

 

Acta Cryst. (2010). D66, 1269-1270  [ doi:10.1107/S0907444910041818 ]

Citations in supplementary material

M. S. Weiss, H. Einspahr, E. N. Baker, Z. Dauter, A. R. Kaysser-Pyzalla, G. Kostorz and S. Larsen

Synopsis: The problem of undercounting of citations that are published only in supplementary material is studied for the journals Nature, Science, Cell and the Proceedings of the National Academy of Sciences (USA).

Online 16 November 2010

 

Acta Cryst. (2010). D66, 1271-1277  [ doi:10.1107/S0907444910035808 ]

Preferential selection of isomer binding from chiral mixtures: alternate binding modes observed for the E and Z isomers of a series of 5-substituted 2,4-diaminofuro[2,3-d]pyrimidines as ternary complexes with NADPH and human dihydrofolate reductase

V. Cody, J. Piraino, J. Pace, W. Li and A. Gangjee

Synopsis: The structures of six chirally mixed E/Z-isomers of 5-substituted 2,4-diaminofuro[2,3-d]pyrimidines reveals only one isomer is bound in the active site of human DHFR. The configuration of all but one C9-analogue is observed as the E-isomer.

PDB references: 3nxo, 3nxr, 3nxt, 3nxv, 3nxx and 3nxy

Online 16 November 2010

 

Acta Cryst. (2010). D66, 1278-1286  [ doi:10.1107/S0907444910036681 ]

The 2.7 Å resolution structure of the glycopeptide sulfotransferase Teg14

M. J. Bick, J. J. Banik, S. A. Darst and S. F. Brady

Synopsis: The 2.7 Å resolution crystal structure of Teg14, a glycopeptide sulfotransferase cloned from an uncultured soil bacterium, is described. The relationship of Teg14 to other sulfotransferases is discussed.

PDB reference: 3nib

Online 16 November 2010

 

Acta Cryst. (2010). D66, 1287-1294  [ doi:10.1107/S0907444910036875 ]

Spatial dependence and mitigation of radiation damage by a line-focus mini-beam

Y. Z. Finfrock, E. A. Stern, Y. Yacoby, R. W. Alkire, K. Evans-Lutterodt, A. Stein, A. F. Isakovic, J. J. Kas and A. Joachimiak

Synopsis: Measurements of the penetration depth and distribution of photoelectron damage excited by 18.6 keV photons in a lysozyme crystal are reported for the first time.

Online 16 November 2010

 

Acta Cryst. (2010). D66, 1295-1300  [ doi:10.1107/S0907444910036966 ]

Role of domains within the autotransporter Hbp/Tsh

K. Nishimura, Y.-H. Yoon, A. Kurihara, S. Unzai, J. Luirink, S.-Y. Park and J. R. H. Tame

Synopsis: The crystal structure of the autotransporter Hbp missing domain 2 is presented. The role of domain 2 is unknown, but domain 1 is shown to bind calcium ions.

PDB reference: 3ak5

Online 16 November 2010

 

Acta Cryst. (2010). D66, 1301-1307  [ doi:10.1107/S0907444910037558 ]

Structure of an archaeal homologue of the bacterial Fmu/RsmB/RrmB rRNA cytosine 5-methyltransferase

Y. Hikida, M. Kuratani, Y. Bessho, S. Sekine and S. Yokoyama

Synopsis: The crystal structure of PH0851, an archaeal homologue of the bacterial Fmu/RsmB/RrmB rRNA cytosine 5-methyltransferase, has been determined.

PDB reference: 2yxl

Online 16 November 2010

 

Acta Cryst. (2010). D66, 1308-1315  [ doi:10.1107/S0907444910039375 ]

Structural and biochemical analyses reveal a monomeric state of the bacterial lipocalin Blc

A. Schiefner, L. Chatwell, D. A. Breustedt and A. Skerra

Synopsis: Crystallographic and biochemical analyses of the recombinant bacterial lipocalin, Blc, provide evidence for its native state as a functional monomer, as for most other natural lipocalins. The previously reported dimeric structure of a modified version of Blc (Blc-X) seems to be a result of an N-terminal extension arising from the applied cloning strategy.

PDB reference: 3mbt

Online 16 November 2010

 

Acta Cryst. (2010). D66, 1316-1322  [ doi:10.1107/S0907444910040370 ]

Structure of a crystal form of human methemoglobin indicative of fiber formation

S. B. Larson, J. S. Day, C. Nguyen, R. Cudney and A. McPherson

Synopsis: A novel form of crystalline human methemoglobin has been solved that has 12 molecules of the protein comprising helical turns of about 675 Å pitch. The possible relationship of this helix to sickle cell hemoglobin fibrils is discussed.

PDB reference: 3odq

Online 16 November 2010

 

Acta Cryst. (2010). D66, 1323-1333  [ doi:10.1107/S0907444910040394 ]

Structural analysis of actinidin and a comparison of cadmium and sulfur anomalous signals from actinidin crystals measured using in-house copper- and chromium-anode X-ray sources

M. Yogavel, N. Nithya, A. Suzuki, Y. Sugiyama, T. Yamane, D. Velmurugan and A. Sharma

Synopsis: The crystal structure of actinidin from A. arguta Planch. (sarunashi) was determined by the SAD method using in-house Cu K and Cr K radiation.

PDB references: 3p5u, 3p5v, 3p5w and 3p5x

Online 20 November 2010

 

Acta Cryst. (2010). D66, 1334-1340  [ doi:10.1107/S0907444910040886 ]

High-resolution structures of Trypanosoma brucei pteridine reductase ligand complexes inform on the placement of new molecular entities in the active site of a potential drug target

A. Dawson, L. B. Tulloch, K. L. Barrack and W. N. Hunter

Synopsis: High-resolution crystal structures of T. brucei pteridine reductase in complex with four ligands are described. The ligands include the known antifolates pemetrexed and trimetrexate, a 2,4-diaminopyrimidine derivative and cyromazine, a melamine derivative of veterinary importance. The structures provide accurate information about new molecular entities in the active site of a potential drug target against protozoan parasites.

PDB references: 2x9n, 2x9g, 2x9v and 3mcv

Online 16 November 2010

 

Acta Cryst. (2010). D66, 1341-1350  [ doi:10.1107/S0907444910040928 ]

On the complexity of Engh and Huber refinement restraints: the angle as example

W. G. Touw and G. Vriend

Synopsis: The angle (backbone N-C-C) is the most contested Engh and Huber refinement target parameter. It is shown that this parameter is `correct' as a PDB-wide average, but can be improved by taking into account residue types, secondary structures and many other aspects of our knowledge of the biophysical relations between residue type and protein structure.

Online 16 November 2010

 

Acta Cryst. (2010). D66, 1351-1357  [ doi:10.1107/S0907444910042228 ]

Structure of the human FOXO4-DBD-DNA complex at 1.9 Å resolution reveals new details of FOXO binding to the DNA

E. Boura, L. Rezabkova, J. Brynda, V. Obsilova and T. Obsil

Synopsis: The 1.9 Å resolution structure of FOXO4-DBD-DNA complex is reported and compared with other known structures of FOXO-DBD-DNA complexes.

PDB reference: 3l2c

Online 16 November 2010