issue contents

Journal logoBIOLOGICAL
ISSN: 1399-0047

August 2011 issue

Highlighted illustration

Cover illustration: Pictorial one-dimensional helical symmetry description of three helical structures determined by X-ray fibre diffraction (p. 716). The three depicted helical structures are the filamentous bacteriophage ph75 (PDB entry 1hgv), F-actin (PDB entry 2zwh) and the cucumber green mottle mosaic virus (PDB entry 1cgm). Individual asymmetric subunits are each represented by a color isosurface entity and each color represents a helix specified by the helical symmetry.

research papers

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The crystal structure of 5-hydroxyisourate hydrolase from K. pneumoniae and the steady-state kinetic parameters of the native enzyme as well as several mutants provide insights into the catalytic mechanism of this enzyme and the possible roles of the active-site residues.

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The crystal structure of 2-oxo-3-deoxygalactonate kinase from the De Ley–Doudoroff pathway of galactose metabolism has been determined at 2.1 Å resolution.

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This study presents the crystal structure of catalase from the thermotolerant yeast H. polymorpha at 2.9 Å resolution.

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A script was created to allow SHELXL to use the new CDL v.1.2 stereochemical library which defines the target values for main-chain bond lengths and angles as a function of the residue's φ/ψ angles. Test refinements using this script show that the refinement behavior of structures at resolutions even better than 1 Å is substantially enhanced by the use of the new conformation-dependent ideal geometry paradigm.

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Purine biosynthesis is considered to be a promising new antibiotic target; in this article, the structural and biochemical characterization of S. aureus PurK and PurE are described.

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A new representation of helical structure by four parameters, [n1, n2, twist, rise], is able to generate an entire helical construct from asymmetric units, including cases of helical assembly with a seam.

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The performance of molecular-replacement approaches can be enhanced by utilizing anomalous differences and substructure information.

short communications

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Application of a highly detailed model for the bulk solvent obtained from molecular-dynamics simulations shows an improvement of the totally cross-validated free R value of only 0.3%.

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An anomalous dispersion analysis was performed in order to conclusively demonstrate the absence of Cl between the Fe3+ and Cu2+ in resting-oxidized cytochrome c oxidase.

addenda and errata

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