Acta Cryst. (2011). D67, 997-1008
[ doi:10.1107/S090744491104039X ]

The structure and inhibition of a GGDEF diguanylate cyclase complexed with (c-di-GMP)₂ at the active site

C.-Y. Yang, K.-H. Chin, M.L.-C. Chuah, Z.-X. Liang, A.H.-J. Wang and S.-H. Chou

Synopsis: The crystal structure of the XCC4471^GGDEF-c-di-GMP complex reveals detailed interactions between a c-di-GMP dimer with a significant number of highly conserved residues around the active site. This structure may serve as a good model for designing lead compounds for blocking DGC activities in a cell and for treating biofilm-related chronic diseases.

PDB reference: 3qyy

Online 18 November 2011

 

Acta Cryst. (2011). D67, 1009-1016
[ doi:10.1107/S0907444911041102 ]

Role of the MotB linker in the assembly and activation of the bacterial flagellar motor

J. O'Neill, M. Xie, M. Hijnen and A. Roujeinikova

Synopsis: The crystallographic analysis of a series of N-terminally truncated H. pylori MotB variants is presented. This analysis reveals how the structure responds to linker separation and suggests the mechanism of MotB activation, which entails linker unfolding and rotational displacement of MotB transmembrane helices.

PDB references: 3s0y, 3s02, 3s03, 3s06, 3s0h and 3s0w

Online 5 November 2011

 

Acta Cryst. (2011). D67, 1017-1022
[ doi:10.1107/S0907444911042971 ]

Structural and functional characterization of Mycobacterium tuberculosis triosephosphate isomerase

S. E. Connor, G. C. Capodagli, M. K. Deaton and S. D. Pegan

Synopsis: The structure of M. tuberculosis triosephosphate isomerase (MtTPI) was elucidated in the apo form as well as bound to the reaction-intermediate analog phosphoglycolohydroxamate (PGH). The enzymatic parameters for the conversion of dihydroxyacetone phosphate to D-glyceraldehyde-3-phosphate by MtTPI were also obtained as well as the binding constant for the reaction-intermediate analog PGH.

PDB references: 3ta6 and 3tao

Online 5 November 2011

 

Acta Cryst. (2011). D67, 1023-1027
[ doi:10.1107/S0907444911043435 ]

Structural analysis of interdomain mobility in ribosomal L1 proteins

S. Tishchenko, E. Nikonova, O. Kostareva, A. Gabdulkhakov, W. Piendl, N. Nevskaya, M. Garber and S. Nikonov

Synopsis: It is shown that interdomain mobility in bacterial ribosomal L1 proteins emerges from their amino-acid sequences and consequently the closed conformation should be characteristic of the structures of all bacterial L1 proteins in the isolated state.

PDB reference: 3tg8

Online 5 November 2011

 

Acta Cryst. (2011). D67, 1028-1034
[ doi:10.1107/S090744491104385X ]

Structure of E69Q mutant of human muscle fructose-1,6-bisphosphatase

M. Zarzycki, R. Kolodziejczyk, E. Maciaszczyk-Dziubinska, R. Wysocki, M. Jaskolski and A. Dzugaj

Synopsis: Human muscle fructose-1,6-bisphosphatase has been characterized in complex with its allosteric inhibitor AMP and in addition with the enzymatic reaction product -fructose 6-phosphate bound in the active site.

PDB references: 3ifa and 3ifc

Online 18 November 2011

 

Acta Cryst. (2011). D67, 1035-1044
[ doi:10.1107/S090744491104491X ]

Structural insights into a zinc-dependent pathway leading to Leu55Pro transthyretin amyloid fibrils

A. F. Castro-Rodrigues, L. Gales, M. J. Saraiva and A. M. Damas

Synopsis: The crystal structure of the TTR L55P-Zn²⁺ complex reveals two different tetrahedral Zn²⁺-binding sites that lead to a bidimensional array of monomers with a cross- structure. Biophysical characterization of TTR L55P fibril formation under conditions similar to those in which crystals were obtained confirms the role of Zn²⁺ as an amyloid enhancer.

PDB reference: 3ssg

Online 5 November 2011

 

Acta Cryst. (2011). D67, 1045-1053
[ doi:10.1107/S0907444911045033 ]

Structural basis for the DNA-binding activity of the bacterial -propeller protein YncE

W. Kagawa, T. Sagawa, H. Niki and H. Kurumizaka

Synopsis: Crystal structures of the E. coli YncE protein and its complex with DNA are presented. The structures provide a novel DNA binding mechanism for -propeller-nucleic acid interactions.

PDB references: 3vgz and 3vh0

Online 5 November 2011

 

Acta Cryst. (2011). D67, 1054-1064
[ doi:10.1107/S0907444911045264 ]

Structure of indole-3-glycerol phosphate synthase from Thermus thermophilus HB8: implications for thermal stability

B. Bagautdinov and K. Yutani

Synopsis: The crystal structure of indole-3-glycerol phosphate synthase (IGPS) from the thermophilic bacterium T. thermophilus was determined at 1.8 Å resolution. The study highlights the presence of additional salt bridges and stabilization-centre clusters as major contributing factors to the thermal stability of the thermophilic IGPS and thus provides new insights for protein engineering to design thermostable proteins.

PDB reference: 1vc4

Online 18 November 2011

 

Acta Cryst. (2011). D67, 1065-1072
[ doi:10.1107/S0907444911046300 ]

The structure of Serratia marcescens Lip, a membrane-bound component of the type VI secretion system

V. A. Rao, S. M. Shepherd, G. English, S. J. Coulthurst and W. N. Hunter

Synopsis: The high-resolution crystal structure of S. marcescens Lip reveals a new member of the transthyretin family of proteins. Lip, a core component of the type VI secretion apparatus, is localized to the outer membrane and is positioned to interact with other proteins forming this complex system.

PDB reference: 4a1r

Online 18 November 2011

 

Acta Cryst. (2011). D67, 1073-1075
[ doi:10.1107/S0907444911046105 ]

Deprotonated imidodiphosphate in AMPPNP-containing protein structures

M. Dauter and Z. Dauter

Synopsis: In certain AMPPNP-containing protein structures, the nitrogen bridging the two terminal phosphate groups can be deprotonated.

Online 18 November 2011

 

Acta Cryst. (2011). D67, 1076
[ doi:10.1107/S0907444911045860 ]

Crystallographic analysis of human hemoglobin elucidates the structural basis of the potent and dual antisickling activity of pyridyl derivatives of vanillin. Corrigendum

O. Abdulmalik, M. S. Ghatge, F. N. Musayev, A. Parikh, Q. Chen, J. Yang, I. Nnamani, R. Danso-Danquah, D. N. Eseonu, T. Asakura, D. J. Abraham, J. Venitz and M. K. Safo

Synopsis: A correction to the paper by Abdulmalik et al. [(2011), Acta Cryst. D67, 920-928].

Online 5 November 2011

 

Acta Cryst. (2011). D67, 1077
[ doi:10.1107/S0907444911048633 ]

The unique structure of carbonic anhydrase CA1 from Chlamydomonas reinhardtii. Corrigendum

K. Suzuki, S.-Y. Yang, S. Shimizu, E. C. Morishita, J. Jiang, F. Zhang, M. M. Hoque, Y. Sato, M. Tsunoda, T. Sekiguchi and A. Takénaka

Synopsis: A correction to the paper by Suzuki et al. [(2011). Acta Cryst. D67, 894-901].

Online 18 November 2011