journal menu
![[Figure 1]](mn5005fig1mag.jpg)
|
|
Figure 1
Detection of Lip–Lip self-interaction. The bacterial two-hybrid system was used to detect an in vivo interaction between Lip (minus signal peptide) fused to T25 (pSC080) and Lip (minus signal peptide) fused to T18 (pSC072). The empty vectors pUT18 and pT25 represent negative controls. The graph shows the output from the two-hybrid system detected as β-galactosidase activity expressed relative to the pUT18/pT25 baseline level (the maximal β-galactosidase activity observed for the Lip–Lip interaction corresponded to >5000 Miller units). Bars show mean ± SEM. Inset: colourimetric readout of the two-hybrid assay following growth of E. coli BTH101 carrying the above plasmids on MacConkey–maltose agar (red is a positive result). |
![[Figure 1]](mn5005fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
Open 
access
access
