Figure 3
Disulfide-bond formation in the purified human Bach2 POZ domain in solution. (a) Analysis of purified wild-type Bach2 POZ-domain protein by SDS-PAGE. The sample in lane 1 contained 20 mM DTT and the samples in lanes 2-8 were treated with various concentrations of hydrogen peroxide for 1 h at room temperature and electrophoresed under nonreducing conditions. (b) Analysis of purified C20S Bach2 POZ-domain protein by SDS-PAGE. Samples were treated as described in (a). (c) Purified wild-type Bach2 POZ-domain protein, together with the C20S, C125S and C20S/C125S mutants, was analysed by SDS-PAGE. Reduced samples were treated with 5% [beta]-mercaptoethanol ([beta]Me). Oxidized samples were incubated with 1 mM hydrogen peroxide for 1 h at room temperature.  [article HTML]

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