Experimental phasing using zinc anomalous scattering
aMarine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea, bDepartment of Marine Biotechnology, University of Science and Technology, Daejeon 305-333, Republic of Korea, and cDepartment of Biological Sciences, KAIST Institute for the Biocentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea
*Correspondence e-mail: firstname.lastname@example.org
Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.
Experimental phasing is essential to solve protein structures which cannot be determined by molecular replacement. The use of tunable synchrotron radiation has led to the development of multiwavelength anomalous dispersion (MAD) and single-wavelength anomalous dispersion (SAD), powerful phasing methods that are based on the anomalous scattering of certain atoms. Selenium is the most commonly used anomalous scatterer for MAD/SAD because selenomethionine and selenocysteine can be incorporated into recombinant proteins. However, selenium-based MAD or SAD is not applicable to natural proteins and is hardly applicable to recombinant proteins with no methionine or cysteine. In addition, the crystallization of selenium-labelled proteins is sometimes unsuccessful despite the successful crystallization of the corresponding native proteins. In these cases, the introduction of anomalous scatterers such as heavy atoms and halide ions into crystals by soaking is an alternative. Heavy-atom derivatives are usually prepared by soaking multiple crystals in numerous heavy-atom compound solutions containing gold, platinum, mercury or lead for days to weeks, while a quick (less than 1 min) soak of crystals in cryosolutions containing bromides or iodides allows the incorporation of these ions (Dauter & Dauter, 1999; Dauter et al., 2000).
Zinc is the second most abundant metal in cells and serves as a cofactor for diverse enzymes and regulatory proteins or contributes to the structural integrity of proteins. It is estimated that about 5–10% of all proteins predicted from the genomes of all three domains of life are zinc-binding proteins (Andreini et al., 2006). Zinc is an ideal anomalous scatterer for MAD/SAD phasing considering that its strong anomalous signal (f″ = 3.9 electrons at its K edge) is comparable to that of Se and its absorption edge (λ = 1.284 Å) falls within the normal energy range of macromolecular crystallography beamlines. Crystal structures of many proteins with intrinsically bound zinc have been successfully determined using zinc anomalous scattering. Nevertheless, zinc has rarely been used to prepare heavy-atom derivatives and no zinc compound is included in commercially available heavy-atom screening kits, which seems to be a consequence of the general notion that specific metal-binding sites are a prerequisite for zinc binding. Here, we show that the surface of proteins can be charged with zinc ions and that the anomalous signals from these zinc ions can be used for structure determination of proteins.
The gene for CMY-10 was chemically synthesized and subsequent subcloning and purification were performed as described previously (Lee et al., 2004). Crystals of CMY-10 were grown in a precipitant solution consisting of 18% polyethylene glycol 8000, 0.1 M sodium cacodylate pH 6.5, 0.2 M zinc acetate dehydrate using the microbatch crystallization method.
The TON_0340 gene of Thermococcus onnurineus NA1 was cloned into pET22b-CPD 10H, an in-house-modified form of pET22b (Novagen), to express a protein fused to His10-tagged CPD (cysteinyl protease domain) at the C-terminus (Shen et al., 2009). The fusion protein was expressed in Escherichia coli BL21 (DE3) RIPL strain (Novagen) at 310 K. Bacterial lysates were prepared by sonication in buffer A composed of 20 mM Tris–HCl pH 7.5, 100 mM NaCl, 5 mM β-mercaptoethanol. Cleared lysates isolated by centrifugation were loaded onto a column packed with HisPur Cobalt Resin (Thermo) and washed with buffer A containing 10 mM imidazole. On-gel auto-cleavage of His10-tagged CPD was performed by incubating the resin with buffer A containing 100 mM phytate for 2 h at room temperature, which activates the protease activity of CPD. TON_0340 was eluted with buffer A and further purified with a HiTrap Q HP column (GE Healthcare). For crystallization, the final sample was concentrated to 9 mg ml−1 in a buffer solution composed of 20 mM Tris–HCl pH 7.5, 100 mM NaCl, 1 mM dithiothreitol. Crystals were obtained by the hanging-drop vapour-diffusion method using a precipitant solution consisting of 5%(v/v) 2-propanol, 100 mM sodium acetate pH 5.0, 350 mM zinc acetate at 295 K.
Hen egg-white lysozyme was purchased from BIO Basic Inc. and was used without further purification. Lysozyme crystals were grown at 295 K using the microbatch crystallization method. Small drops composed of 1 µl protein solution (20–50 mg ml−1) and an equal volume of a precipitant solution consisting of 1 M NaCl, 0.1 M citric acid pH 4.0 were pipetted under a layer of a 1:1 mixture of silicon oil and paraffin oil in 72-well HLA plates (Nunc).
A 2.1 Å resolution MAD data set for CMY-10 was collected at wavelengths of 1.2825 Å (peak), 1.2828 Å (inflection point) and 1.1700 Å (high-energy remote) using an ADSC Quantum 270 CCD on the microfocus beamline PF-17A at the Photon Factory, Japan. For each data set, 180 diffraction images with a 1° oscillation width were collected with the crystal-to-detector distance set to 180 mm (Table 1).
‡Data from SHELXC (Sheldrick, 2010).
§Data from phasing program.
¶Rwork = . Rfree was calculated using 5% of the reflections.
††R.m.s. deviations in bond length and angles are the deviations from ideal values.
A 2.3 Å resolution SAD data set for TON_0340 was collected at the zinc absorption peak using an ADSC Quantum 315 CCD on beamline 4A of Pohang Light Source, Republic of Korea (Table 1). In order to avoid spot overlaps arising from the long c axis of the unit cell, we used a 90°-bent metal pin to align the c axis along the spindle axis and collected 200 images of 1° oscillation with the crystal-to-detector distance set to 300 mm (covering 200° of oscillation) that are free of interference from the metal pin (Table 1).
A 1.8 Å resolution SAD data set for hen egg-white lysozyme was collected at a wavelength of 1.2829 Å using an ADSC Quantum 270 CCD on the microfocus beamline PF-17A at the Photon Factory, Japan. A total of 180 frames of 1° oscillation were collected with the crystal-to-detector distance set to 176 mm (Table 1).
Diffraction data were processed and scaled using DENZO and SCALEPACK from the HKL-2000 program suite (Otwinowski & Minor, 1997). Experimental phasing of CMY-10 and lysozyme was performed with the AutoSol program (Terwilliger et al., 2009) in the PHENIX suite (Adams et al., 2010), which is an experimental phasing pipeline that combines HySS (Hybrid Substructure Search; Grosse-Kunstleve & Adams, 2003) for finding heavy-atom sites, Phaser (McCoy et al., 2007) or SOLVE (Terwilliger, 2002) for calculating experimental phases and RESOLVE (Terwilliger, 2002) for density modification and model building. Experimental phasing of TON_0340 was performed with autoSHARP (Vonrhein et al., 2007), an automatic structure-solution system that includes the heavy-atom refinement and phasing program SHARP (de La Fortelle & Bricogne, 1997), the density-modification program SOLOMON (Abrahams & Leslie, 1996) and the ARP/wARP package (Perrakis et al., 1999) for automated model building and refinement. The auto-built models from the phasing programs were completed using Coot (Emsley & Cowtan, 2004) and refinement was performed with a maximum-likelihood algorithm implemented in CNS (Brünger et al., 1998).
We encountered a paper describing zinc binding to a protein without an intrinsic zinc-binding site, the crystals of which grew in a precipitant solution containing zinc acetate (Axelrod et al., 2010). According to the crystal structure that was determined based on selenium anomalous scattering (PDB entry 3h50 ), four zinc ions were bound to surface-exposed aspartate, glutamate and histidine residues. The presence of zinc ions on the protein surface was reminiscent of the crystal structure of CMY-10 (PDB entry 1zkj ) determined previously (Kim et al., 2006). The crystals of this protein also grew in the presence of zinc acetate (Lee et al., 2004) and its crystal structure contained zinc ions tightly associated with the imidazole ring of histidine, the carboxyl group of aspartate/glutamate or the amide group of asparagine/glutamine on the protein surface. In the two crystal structures most zinc ions bound to histidine residues, indicating that histidine is the preferred residue for zinc coordination on the protein surface. The zinc ions were coordinated in a tetrahedral geometry, except for one that was bound to asparagine with an octahedral geometry. Interestingly, a single amino-acid residue was sufficient to bind a zinc ion because water or acetate molecules also participated in the coordination.
3.2. Structure determination of CMY-10 using zinc anomalous scattering
We tested whether anomalous signal from the zinc ions bound to the surface of CMY-10 might be sufficient for phase determination. A 2.1 Å resolution MAD data set (Table 1) was collected at three wavelengths from a crystal that was cooled in a cryostream at 100 K after briefly being immersed in a cryoprotectant solution consisting of 15% glucose, 18%(w/v) polyethylene glycol 8000, 0.1 M sodium cacodylate pH 6.5, 0.2 M zinc acetate dehydrate. The CMY-10 crystals, which contained one molecule per asymmetric unit, belonged to the monoclinic space group P21, with unit-cell parameters a = 49.6, b = 59.2, c = 63.5 Å, β = 103.7°. Anomalous signal, which was evaluated using 〈d″/sig〉, a good indicator of the strength of the anomalous signal, was present to 2.1 Å resolution in all three data sets and was strongest for the peak data set, as expected (Fig. 1). The AutoSol program identified 13 zinc sites, with occupancies in the range 0.19–0.99, and produced a phase set with a figure of merit (FOM) of 0.45. The phases were further improved by density modification, with a final FOM of 0.62. The experimental electron-density map was very clear (Fig. 2) and a model with Rwork/Rfree of 0.2501/0.2784 was automatically built. These results indicate that surface-bound zinc ions can successfully be used for phase determination by the MAD method.
3.3. Structure determination of TON_0340 using zinc anomalous scattering
Recently, we obtained crystals of TON_0340 in a precipitant solution containing zinc acetate. Based on our confidence in zinc derivatization and phase determination, we collected a 2.3 Å resolution SAD data set (Table 1) from a crystal that was cooled in a cryostream at 100 K after briefly being immersed in a cryoprotectant solution consisting of 20% glycerol, 5%(v/v) 2-propanol, 100 mM sodium acetate pH 5.0, 350 mM zinc acetate. The TON_0340 crystals, which contained six molecules per asymmetric unit, belonged to the tetragonal space group P43212, with unit-cell parameters a = b = 107.44, c = 355.03 Å. According to the plot of 〈d″/sig〉 versus resolution, zinc anomalous signal was present, although it decreased drastically at high resolution (Fig. 1). A total of 66 zinc ions, with occupancies of 0.08–1.00, were identified in the asymmetric unit and the resulting phasing set, characterized by an FOM of 0.33, yielded a clearly interpretable electron-density map (Fig. 2) leading to an auto-built model with Rwork/Rfree of 0.27/0.31. Just like the other cases described above, zinc ions were associated with aspartates, glutamates and histidines on the protein surface. Additionally, three zinc ions bound to a cluster of six acidic residues were located in a putative active-site cleft.
3.4. Structure determination of lysozyme using zinc anomalous scattering
In the two cases described above, zinc ions were present in the crystallization solutions. We tested whether the surface of a protein can be charged with zinc ions by soaking protein crystals in a solution containing zinc ions. Crystals of hen egg-white lysozyme were first obtained in a precipitant solution composed of 1 M NaCl, 0.1 M citrate pH 4.0. The lysozyme crystals were then transferred into a solution consisting of 50 mM zinc acetate, 1 M NaCl, 0.1 M MES pH 6.5, 25% ethylene glycol. We changed the pH from 4.0 to 6.5 to deprotonate histidine, which appears to be the most preferred residue to associate with zinc ions. After 10 min soaking, a lysozyme crystal was mounted and the crystal was flash-cooled at 100 K using a cryostream cooler. A fluorescence scan was performed to locate the Zn K edge and a 1.8 Å resolution SAD data set was collected at a wavelength of 1.2829 Å (Table 1). The lysozyme crystal, which contained one molecule per asymmetric unit, belonged to the tetragonal space group P43212, with unit-cell parameters a = b = 79.19, c = 36.82 Å. The anomalous signal was high over the entire resolution range (Fig. 1).
The experimental phase information resulting from the SAD phasing was of high quality; the FOMs before and after density modification were 0.42 and 0.64, respectively. The electron-density map calculated from the experimental phases was readily interpretable (Fig. 2) and a nearly complete model with Rwork/Rfree of 0.1856/0.2323 was automatically built. The root-mean-square deviation for all atoms between zinc-bound lysozyme and zinc-free lysozyme (PDB entry 2lyz ; Diamond, 1974) was only 0.85 Å, indicating that zinc binding had no effect on the structure. Although 21 zinc ions were identified by the phasing program, three zinc ions (Zn1, Zn2 and Zn3) were finally modelled (Table 1). Zn1 was coordinated by Asp52 and three water molecules in a tetrahedral geometry, whereas Zn2 was coordinated by His15 and five water molecules in an octahedral geometry (Fig. 3). In the case of Zn3, the zinc ion interacted with four water molecules in a tetrahedral geometry and two of the coordinating water molecules were engaged in weak hydrogen bonds to backbone N atoms (Fig. 3). The weak association of Zn3 was reflected in its B factor (56.86 Å2), which was higher than those of Zn1 (31.92 Å2) and Zn2 (38.63 Å2). The successful zinc SAD phasing suggests that zinc derivatization of protein surfaces by crystal soaking is a method of choice for de novo structure determination of proteins for which crystals grow in the absence of zinc ions.
We observed that zinc ions derived from crystallization solutions bind to surface-exposed residues. To ascertain the generality of this observation, we searched the Protein Data Bank for proteins for which crystals were grown in solutions containing zinc acetate. The search was stopped after the collection of 43 cases (see Supplementary Material1) because all the cases were consistent with our observations. Without exception, zinc ions are associated with surface residues such as histidine, aspartate, glutamate, asparagine or glutamine. Among the collected cases, we found that three structures were solved by Zn MAD phasing [PDB entries 1fd9 (Riboldi-Tunnicliffe et al., 2001), 2ch9 (Schüttelkopf et al., 2006) and 3cjj (Koch et al., 2010)]. The three proteins are not zinc-binding proteins, but the phasing method was tried because zinc ions were essential for crystallization. There are two, four and five zinc ions in the 1fd9 , 3cjj and 2ch9 structures, respectively, and their occupancies range from 0.2 to 1. While other protein structures were determined by molecular replacement or selenium-based MAD/SAD phasing, our experimental demonstrations suggest that their structures could have been determined by exploiting the zinc anomalous signal. A survey of the crystallization conditions of the 43 cases showed that the concentration of zinc acetate ranged from 3 to 300 mM, indicating that zinc-bound crystals can be prepared at diverse zinc concentrations. The buffer pH range of 4.5–8.3 revealed by this survey might indicate the limit of zinc-binding conditions since zinc-binding residues are protonated at low pH and zinc in solution is precipitated at high pH.
In conclusion, we demonstrated that protein crystals grown in the presence of zinc ions or soaked in a zinc-containing solution are easily charged with multiple zinc ions on the protein surface and that they are suitable for zinc SAD/MAD phasing. A phasing effectiveness of 570 amino acids per fully occupied Zn atom was shown experimentally and simulated MAD phasing suggested that one Zn site is able to provide adequate experimental phase information for as many as 1100 amino acids (Meyer et al., 2006). Considering the high phasing effectiveness and multiple zinc-binding sites, we believe that zinc derivatization of the protein surface is a largely unnoticed but promising method for phase determination of protein crystals.
This work was supported by National Research Foundation of Korea Grant 2012005978, the `National Agenda Program' through KRCF, KIST and KORDI, the Marine and Extreme Genome Research Center program and the Development of Biohydrogen Production Technology Using Hyperthermophilic Archaea program of MLTM, and the KORDI in-house NSC program.
Abrahams, J. P. & Leslie, A. G. W. (1996). Acta Cryst. D52, 30–42. CrossRef CAS Web of Science IUCr Journals
Adams, P. D. et al. (2010). Acta Cryst. D66, 213–221. Web of Science CrossRef CAS IUCr Journals
Ahmed, A. H., Ptak, C. P. & Oswald, R. E. (2010). Biochemistry, 49, 2843–2850. Web of Science CrossRef CAS PubMed
Ahmed, A. H., Wang, Q., Sondermann, H. & Oswald, R. E. (2009). Proteins, 75, 628–637. Web of Science CrossRef PubMed CAS
Andreini, C., Banci, L., Bertini, I. & Rosato, A. (2006). J. Proteome Res. 5, 3173–3178. Web of Science CrossRef PubMed CAS
Axelrod, H. L. et al. (2010). Acta Cryst. F66, 1347–1353. Web of Science CrossRef CAS IUCr Journals
Brünger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J.-S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T. & Warren, G. L. (1998). Acta Cryst. D54, 905–921. Web of Science CrossRef IUCr Journals
Cho, Y., Vermeire, J. J., Merkel, J. S., Leng, L., Du, X., Bucala, R., Cappello, M. & Lolis, E. (2011). Chem. Biol. 18, 1089–1101. Web of Science CrossRef CAS PubMed
Dauter, Z. & Dauter, M. (1999). J. Mol. Biol. 289, 93–101. Web of Science CrossRef PubMed CAS
Dauter, Z., Dauter, M. & Rajashankar, K. R. (2000). Acta Cryst. D56, 232–237. Web of Science CrossRef CAS IUCr Journals
Diamond, R. (1974). J. Mol. Biol. 82, 371–391. CrossRef CAS PubMed Web of Science
Doré, A. S., Kilkenny, M. L., Jones, S. A., Oliver, A. W., Roe, S. M., Bell, S. D. & Pearl, L. H. (2006). Nucleic Acids Res. 34, 4515–4526. Web of Science PubMed
Duquerroy, S., Stura, E. A., Bressanelli, S., Fabiane, S. M., Vaney, M. C., Beale, D., Hamon, M., Casali, P., Rey, F. A., Sutton, B. J. & Taussig, M. J. (2007). J. Mol. Biol. 368, 1321–1331. Web of Science CrossRef PubMed CAS
Emsley, P. & Cowtan, K. (2004). Acta Cryst. D60, 2126–2132. Web of Science CrossRef CAS IUCr Journals
Fieulaine, S., Boularot, A., Artaud, I., Desmadril, M., Dardel, F., Meinnel, T. & Giglione, C. (2011). PLoS Biol. 9, e1001066. Web of Science CrossRef PubMed
Grosse-Kunstleve, R. W. & Adams, P. D. (2003). Acta Cryst. D59, 1966–1973. Web of Science CrossRef CAS IUCr Journals
Hald, H., Ahring, P. K., Timmermann, D. B., Liljefors, T., Gajhede, M. & Kastrup, J. S. (2009). J. Mol. Biol. 391, 906–917. Web of Science CrossRef PubMed CAS
Inuzuka, T., Suzuki, H., Kawasaki, M., Shibata, H., Wakatsuki, S. & Maki, M. (2010). BMC Struct. Biol. 10, 25.
Jin, R., Clark, S., Weeks, A. M., Dudman, J. T., Gouaux, E. & Partin, K. M. (2005). J. Neurosci. 25, 9027–9036. Web of Science CrossRef PubMed CAS
Kim, M. W., Chelliah, Y., Kim, S. W., Otwinowski, Z. & Bezprozvanny, I. (2009). Structure, 17, 1205–1212. Web of Science CrossRef PubMed CAS
Kim, J. Y., Jung, H. I., An, Y. J., Lee, J. H., Kim, S. J., Jeong, S. H., Lee, K. J., Suh, P.-G., Lee, H.-S., Lee, S. H. & Cha, S.-S. (2006). Mol. Microbiol. 60, 907–916. Web of Science CrossRef PubMed CAS
Koch, M., Chitayat, S., Dattilo, B. M., Schiefner, A., Diez, J., Chazin, W. J. & Fritz, G. (2010). Structure, 18, 1342–1352. Web of Science CrossRef CAS PubMed
Komander, D., Reyes-Turcu, F., Licchesi, J. D., Odenwaelder, P., Wilkinson, K. D. & Barford, D. (2009). EMBO Rep. 10, 466–473. Web of Science CrossRef PubMed CAS
La Fortelle, E. de & Bricogne, G. (1997). Methods Enzymol. 276, 472–494.
Lee, S.-J., Kim, J. Y., Jung, H. I., Suh, P.-G., Lee, H.-S., Lee, S. H. & Cha, S.-S. (2004). Acta Cryst. D60, 382–384. Web of Science CrossRef CAS IUCr Journals
Lunn, M. L., Hogner, A., Stensbøl, T. B., Gouaux, E., Egebjerg, J. & Kastrup, J. S. (2003). J. Med. Chem. 46, 872–875. Web of Science CrossRef PubMed CAS
Makino, C. L., Riley, C. K., Looney, J., Crouch, R. K. & Okada, T. (2010). Biophys. J. 99, 2366–2373. Web of Science CrossRef CAS PubMed
McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C. & Read, R. J. (2007). J. Appl. Cryst. 40, 658–674. Web of Science CrossRef CAS IUCr Journals
McRobbie, A. M., Carter, L. G., Kerou, M., Liu, H., McMahon, S. A., Johnson, K. A., Oke, M., Naismith, J. H. & White, M. F. (2009). J. Mol. Biol. 389, 661–673. Web of Science CrossRef PubMed CAS
Meyer, P. A., Ye, P., Zhang, M., Suh, M.-H. & Fu, J. (2006). Structure, 14, 973–982. Web of Science CrossRef PubMed CAS
Nakayama, D., Ben Ammar, Y., Miyata, T. & Takeda, S. (2011). FEBS Lett. 585, 3020–3025. Web of Science CrossRef CAS PubMed
O'Neill, J. W., Kim, D. E., Johnsen, K., Baker, D. & Zhang, K. Y. J. (2001). Structure, 9, 1017–1027. Web of Science PubMed CAS
Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307–326. CrossRef CAS Web of Science
Perrakis, A., Morris, R. & Lamzin, V. S. (1999). Nature Struct. Biol. 6, 458–463. Web of Science CrossRef PubMed CAS
Poon, K., Ahmed, A. H., Nowak, L. M. & Oswald, R. E. (2011). Mol. Pharmacol. 80, 49–59. Web of Science CrossRef CAS PubMed
Ren, X. & Hurley, J. H. (2011). EMBO J. 30, 2130–2139. Web of Science CrossRef CAS PubMed
Riboldi-Tunnicliffe, A., König, B., Jessen, S., Weiss, M. S., Rahfeld, J., Hacker, J., Fischer, G. & Hilgenfeld, R. (2001). Nature Struct. Biol. 8, 779–783. Web of Science PubMed CAS
Sanglas, L., Aviles, F. X., Huber, R., Gomis-Rüth, F. X. & Arolas, J. L. (2009). Proc. Natl Acad. Sci. USA, 106, 1743–1747. Web of Science CrossRef PubMed CAS
Schüttelkopf, A. W., Hamilton, G., Watts, C. & van Aalten, D. M. (2006). J. Biol. Chem. 281, 16570–16575. Web of Science PubMed
Sheldrick, G. M. (2010). Acta Cryst. D66, 479–485. Web of Science CrossRef CAS IUCr Journals
Shen, A., Lupardus, P. J., Morell, M., Ponder, E. L., Sadaghiani, A. M., Garcia, K. C. & Bogyo, M. (2009). PLoS One, 4, e8119. Web of Science CrossRef PubMed
Stamp, A. L., Owen, P., El Omari, K., Lockyer, M., Lamb, H. K., Charles, I. G., Hawkins, A. R. & Stammers, D. K. (2011). Proteins, 79, 2352–2357. Web of Science CrossRef CAS PubMed
Stogios, P. J., Cuesta-Seijo, J. A., Chen, L., Pomroy, N. C. & Privé, G. G. (2010). J. Mol. Biol. 400, 983–997. Web of Science CrossRef CAS PubMed
Terwilliger, T. C. (2002). Acta Cryst. D58, 1937–1940. Web of Science CrossRef CAS IUCr Journals
Terwilliger, T. C., Adams, P. D., Read, R. J., McCoy, A. J., Moriarty, N. W., Grosse-Kunstleve, R. W., Afonine, P. V., Zwart, P. H. & Hung, L.-W. (2009). Acta Cryst. D65, 582–601. Web of Science CrossRef CAS IUCr Journals
Touw, D. S., Nordman, C. E., Stuckey, J. A. & Pecoraro, V. L. (2007). Proc. Natl Acad. Sci. USA, 104, 11969–11974. Web of Science CrossRef PubMed CAS
Virdee, S., Ye, Y., Nguyen, D. P., Komander, D. & Chin, J. W. (2010). Nature Chem. Biol. 6, 750–757. Web of Science CrossRef CAS
Vonrhein, C., Blanc, E., Roversi, P. & Bricogne, G. (2007). Methods Mol. Biol. 364, 215–230. PubMed CAS
Weston, M. C., Gertler, C., Mayer, M. L. & Rosenmund, C. (2006). J. Neurosci. 26, 7650–7658. Web of Science CrossRef PubMed CAS
Xie, P. T. & Hurley, T. D. (1999). Protein Sci. 8, 2639–2644. CrossRef PubMed CAS
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