Structures of an active-site mutant of a plant 1,3-β-glucanase in complex with oligosaccharide products of hydrolysis

Wojtkowiak, A.; Witek, K.; Hennig, J.; Jaskolski, M.

doi:10.1107/S0907444912042175

Figure 3
MALDI-TOF ionization mass-spectrometric analysis of the hydrolysis products of a hexasaccharide (1 [rightwards arrow]

3)-

-glucan catalyzed by the E259A mutant of GLUB20-2. M6, M5, M4 and M3, molecular masses of ionic adducts of Na⁺ and hexasaccharides, pentasaccharides, tetrasaccharides and trisaccharides, respectively; *, matrix ions (DHB, 2,5-dihydroxybenzoic acid; THAP, 2',4',6'-trihydroxyacetophenone).