Acta Crystallographica Section D

Biological Crystallography

Volume 69, Part 3 (March 2013)

research papers

Acta Cryst. (2013). D69, 381-387    [ doi:10.1107/S0907444912048019 ]

REACH: Robotic Equipment for Automated Crystal Harvesting using a six-axis robot arm and a micro-gripper

M. Y. Heidari Khajepour, X. Vernede, D. Cobessi, H. Lebrette, P. Rogues, M. Terrien, C. Berzin and J.-L. Ferrer

Abstract: In protein crystallography experiments, only two critical steps remain manual: the transfer of crystals from their original crystallization drop into the cryoprotection solution followed by flash-cooling. These steps are risky and tedious, requiring a high degree of manual dexterity. These limiting steps are a real bottleneck to high-throughput crystallography and limit the remote use of protein crystallography core facilities. To eliminate this limit, the Robotic Equipment for Automated Crystal Harvesting (REACH) was developed. This robotized system, equipped with a two-finger micro-gripping device, allows crystal harvesting, cryoprotection and flash-cooling. Using this setup, harvesting experiments were performed on several crystals, followed by direct data collection using the same robot arm as a goniometer. Analysis of the diffraction data demonstrates that REACH is highly reliable and efficient and does not alter crystallographic data. This new instrument fills the gap in the high-throughput crystallo­graphic pipeline.

Keywords: REACH; crystal harvesting; cryoprotection; flash-cooling.

pdfdisplay filedownload file

Portable Document Format (PDF) file (558.0 kbytes)
[ doi:10.1107/S0907444912048019/tz5012sup1.pdf ]
Supplementary material


To open or display or play some files, you may need to set your browser up to use the appropriate software. See the full list of file types for an explanation of the different file types and their related mime types and, where available links to sites from where the appropriate software may be obtained.

The download button will force most browsers to prompt for a file name to store the data on your hard disk.

Where possible, images are represented by thumbnails.

 bibliographic record in  format