Acta Crystallographica Section D

Biological Crystallography

Volume 69, Part 5 (May 2013)


integrative structural biology



Acta Cryst. (2013). D69, 920-923    [ doi:10.1107/S0907444913002412 ]

Using high-throughput in situ plate screening to evaluate the effect of dehydration on protein crystals

A. Douangamath, P. Aller, P. Lukacik, J. Sanchez-Weatherby, I. Moraes and J. Brandao-Neto

Abstract: Crystal dehydration is a post-crystallization technique that can potentially improve the diffraction of macromolecular crystals. There are currently several ways of undertaking this process; however, dehydration experiments are often limited in their throughput and require prior manipulation of the samples. In the present study, a novel method is proposed that uses in situ plate screening to assess the effect of dehydration by combining the throughput of 96-well crystallization plates with direct X-ray feedback on crystal diffraction quality.

Keywords: high throughput; in situ plate screening; dehydration.


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[ doi:10.1107/S0907444913002412/tz5020sup1.pdf ]
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