Acta Crystallographica Section D

Biological Crystallography

Volume 69, Part 8 (August 2013)


research papers



Acta Cryst. (2013). D69, 1522-1529    [ doi:10.1107/S0907444913010457 ]

Structural basis for the dephosphorylating activity of PTPRQ towards phosphatidylinositide substrates

K. R. Yu, Y. J. Kim, S.-K. Jung, B. Ku, H. Park, S. Y. Cho, H. Jung, S. J. Chung, K. H. Bae, S. C. Lee, B. Y. Kim, R. L. Erikson, S. E. Ryu and S. J. Kim

Abstract: Unlike other classical protein tyrosine phosphatases (PTPs), PTPRQ (PTP receptor type Q) has dephosphorylating activity towards phosphatidylinositide (PI) substrates. Here, the structure of the catalytic domain of PTPRQ was solved at 1.56 Å resolution. Overall, PTPRQ adopts a tertiary fold typical of other classical PTPs. However, the disordered M6 loop of PTPRQ surrounding the catalytic core and the concomitant absence of interactions of this loop with residues in the PTP loop results in a flat active-site pocket. On the basis of structural and biochemical analyses, it is proposed that this structural feature might facilitate the accommodation of large substrates, making it suitable for the dephosphorylation of PI substrates. Moreover, subsequent kinetic experiments showed that PTPRQ has a strong preferences for PI(3,4,5)P3 over other PI substrates, suggesting that its regulation of cell survival and proliferation reflects downregulation of Akt signalling.

PDB reference: 4ikc

Keywords: PTP receptor type Q; protein tyrosine phosphatases.


pdfdisplay filedownload file

Portable Document Format (PDF) file
[ doi:10.1107/S0907444913010457/dw5043sup1.pdf ]
Supplementary material


Notes:

To open or display or play some files, you may need to set your browser up to use the appropriate software. See the full list of file types for an explanation of the different file types and their related mime types and, where available links to sites from where the appropriate software may be obtained.

The download button will force most browsers to prompt for a file name to store the data on your hard disk.

Where possible, images are represented by thumbnails.

 bibliographic record in  format

  Find reference:   Volume   Page   
  Search:     From   to      Advanced search

Copyright © International Union of Crystallography
IUCr Webmaster