Figure 4
(a) Lanes from SDS-PAGE of cysteine mutants of DnaJ[Delta]108-114 without (-) and with (+) cross-linking by BMB after the removal of tetramers by gel filtration (note that the lanes marked * are the same marker lane shown twice for clarity). (b) Luciferase-refolding activity of DnaK assisted by DnaJ. DnaJ variants with mutations in the GF domain show particularly strong modulation of refolding activity. Experiments were conducted in triplicate.  [article HTML]