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Figure 2
Overview of the HAG method. (a) The humidity-control apparatus: 1, design of the apparatus; 2, the humid air nozzle and the sample; 3, the configuration of the two-flow humidity generator. The apparatus was assembled from the following components: i, digital mass-flow controller; ii, temperature-controlled air-bubbling bottle (2 l); iii, air-cooling bottles (2 l and 500 ml); iv, air-mixing bottle (100 ml); v, humid air nozzle; vi, temperature and humidity sensor; vii, PC to control and monitor humidity through i and ii; viii, cryostream nozzle; ix, crystal sample. (b) Crystal-handling procedures: 1, applying the PVA glue to a cryoloop; 2, picking up a crystal; 3, steeping the crystal to be coated by the glue. (c) A mounted crystal: 1, just before the crystal was placed on the apparatus; 2, after exposure to air of 73.9% RH.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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