Acta Crystallographica Section D

Biological Crystallography

Volume 69, Part 10 (October 2013)


research papers



Acta Cryst. (2013). D69, 2027-2038    [ doi:10.1107/S090744491301799X ]

The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase

P. Zhou, Z. Chen, Q. Yan, S. Yang, R. Hilgenfeld and Z. Jiang

Abstract: Endo-[beta]-1,3-glucanases catalyze the hydrolysis of [beta]-1,3-glycosidic linkages in glucans. They are also responsible for rather diverse physiological functions such as carbon utilization, cell-wall organization and pathogen defence. Glycoside hydrolase (GH) family 81 mainly consists of [beta]-1,3-glucanases from fungi, higher plants and bacteria. A novel GH family 81 [beta]-1,3-glucanase gene (RmLam81A) from Rhizomucor miehei was expressed in Escherichia coli. Purified RmLam81A was crystallized and the structure was determined in two crystal forms (form I-free and form II-Se) at 2.3 and 2.0 Å resolution, respectively. Here, the crystal structure of a member of GH family 81 is reported for the first time. The structure of RmLam81A is greatly different from all endo-[beta]-1,3-glucanase structures available in the Protein Data Bank. The overall structure of the RmLam81A monomer consists of an N-terminal [beta]-sandwich domain, a C-terminal ([alpha]/[alpha])6 domain and an additional domain between them. Glu553 and Glu557 are proposed to serve as the proton donor and basic catalyst, respectively, in a single-displacement mechanism. In addition, Tyr386, Tyr482 and Ser554 possibly contribute to both the position or the ionization state of the basic catalyst Glu557. The first crystal structure of a GH family 81 member will be helpful in the study of the GH family 81 proteins and endo-[beta]-1,3-glucanases.

PDB references: 4k3a and 4k35

Keywords: glycoside hydrolases; family 81; endo-[beta]-1,3-glucanases; Rhizomucor miehei; three-dimensional structure.


pdfdisplay filedownload file

Portable Document Format (PDF) file
[ doi:10.1107/S090744491301799X/dz5286sup1.pdf ]
Supplementary material


Notes:

To open or display or play some files, you may need to set your browser up to use the appropriate software. See the full list of file types for an explanation of the different file types and their related mime types and, where available links to sites from where the appropriate software may be obtained.

The download button will force most browsers to prompt for a file name to store the data on your hard disk.

Where possible, images are represented by thumbnails.

 bibliographic record in  format

  Find reference:   Volume   Page   
  Search:     From   to      Advanced search

Copyright © International Union of Crystallography
IUCr Webmaster