Acta Crystallographica Section D

Biological Crystallography

Volume 69, Part 10 (October 2013)


research papers



Acta Cryst. (2013). D69, 2008-2016    [ doi:10.1107/S0907444913017046 ]

High-resolution structure of an atypical [alpha]-phosphoglucomutase related to eukaryotic phosphomannomutases

P. Nogly, P. M. Matias, M. de Rosa, R. Castro, H. Santos, A. R. Neves and M. Archer

Abstract: The first structure of a bacterial [alpha]-phosphoglucomutase with an overall fold similar to eukaryotic phosphomannomutases is reported. Unlike most [alpha]-phosphoglucomutases within the [alpha]-D-phosphohexomutase superfamily, it belongs to subclass IIb of the haloacid dehalogenase superfamily (HADSF). It catalyzes the reversible conversion of [alpha]-glucose 1-phosphate to glucose 6-phosphate. The crystal structure of [alpha]-phosphoglucomutase from Lactococcus lactis (APGM) was determined at 1.5 Å resolution and contains a sulfate and a glycerol bound at the enzyme active site that partially mimic the substrate. A dimeric form of APGM is present in the crystal and in solution, an arrangement that may be functionally relevant. The catalytic mechanism of APGM and its strict specificity towards [alpha]-glucose 1-phosphate are discussed.

PDB reference: 4bnd

Keywords: [alpha]-phosphoglucomutases; haloacid dehalogenase superfamily; Lactococcus lactis; phosphomannomutases; [alpha]-glucose 1-phosphate; eukaryotic phosphomannomutases; sugar metabolism.


pdfdisplay filedownload file

Portable Document Format (PDF) file
[ doi:10.1107/S0907444913017046/dz5291sup1.pdf ]
Supplementary material


Notes:

To open or display or play some files, you may need to set your browser up to use the appropriate software. See the full list of file types for an explanation of the different file types and their related mime types and, where available links to sites from where the appropriate software may be obtained.

The download button will force most browsers to prompt for a file name to store the data on your hard disk.

Where possible, images are represented by thumbnails.

 bibliographic record in  format

  Find reference:   Volume   Page   
  Search:     From   to      Advanced search

Copyright © International Union of Crystallography
IUCr Webmaster