Figure 3
Three-dimensional structures of mCherry and sfCherry. (a) Structure of mCherry (left; PDB entry 2h5q ; Shu et al., 2006BB55) and sfCherry (right; this work) showing the locations of sfCherry mutations and the corresponding residues in mCherry. (b) Region of mCherry and sfCherry close to residues 147 and 196 showing the hydrogen bonds formed between Asp196 and Thr147, and between Asp196 and Arg220 in the dimeric sfCherry structure (right) and the lack of corresponding hydrogen bonds between Asn196 and Ser147 or Arg220 in the monomeric mCherry structure (left). The images were created with PyMOL (DeLano, 2002BB15)  [article HTML]