Figure 5
A 2mFo - DFc [sigma]A-weighted electron-density map (Winn et al., 2011BB12) was calculated using a model that was constructed before any connections between sfCherry and the GFP(10-11) hairpin had been built. The connections between the green arrows (not included in the phasing model) are between residues 168 and 172 and residues 205 and 211. This unbiased map (contoured at 0.5[sigma] in gray and at 1[sigma] in blue) shows clear connections between sfCherry and the GFP(10-11) hairpin that was inserted into sfCherry at an exposed loop.  [article HTML]