Figure 6
LC/MS analysis. (a) Schematic representation of the enzymatic reaction of Rv3717 towards N-acetylmuramoyl-L-alanyl-D-isoglutamine (M) resulting in products M1 (muramic acid; molecular mass 293.27 Da) and M2 (L-alanyl-D-isoglutamine (molecular mass 217.26 Da), which were analyzed by LC/MS. (b) The reaction mixture was purified by ultrahigh-pressure liquid chromatography (UPLC) into separate peaks of L-alanyl-D-isoglutamine (2.25 min) and muramic acid (2.72 min). (c) MS analysis of the UPLC-separated peaks corresponding to L-alanyl-D-isoglutamine (2.25 min; top panel) and muramic acid (2.72 min; bottom panel).  [article HTML]