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Figure 5
Cleavage assays of CASP6 pro-domain-related variants with the RE mutation. Coomassie Blue-stained SDS–PAGE of (a) N17CASP6(M19A,C163A,RE), (b) N18CASP6(M19A,C163A,RE), (c) N19CASP6(M19F,C163A,RE), (d) N19CASP6(M19A,C163A,RE), (e) N20CASP6(C163A,RE), (f) proCASP6(E17A,C163A,RE), (g) proCASP6(N18A,C163A,RE) and (h) proCASP6(N18L,C163A,RE) incubated with 1% active CASP6 for 30 min. RE, R(64,220)E; FL, full length; p20, large subunit; L, intersubunit linker; p10, small subunit.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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