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Figure 7
Overview of the states captured for ClpB NBD2. 2mFo − DFc electron density (contour levels are given below) is shown for relevant parts of each structure, such as the bound nucleotide and conserved Walker or sensor residues. Mg2+ ions are shown in yellow, including their octahedral coordination sphere with d(Mg–O) = 2.0–2.3 Å. Water molecules are shown as red spheres. Distances are given in Å. (a) Wild-type ClpB NBD2, nucleotide-free, PDB entry 4lj4 , contour level 1.1σ. (b) Wild-type ClpB NBD2 in complex with ADP, PDB entry 4lj5 , contour level 1.5σ. (c) Wild-type ClpB NBD2 in complex with AMPPCP, PDB entry 4lj6 , contour level 1.5σ. (d) ClpB NBD2 K601Q in complex with MANT-dADP, PDB entry 4lj7 , contour level 1.1σ. (e) ClpB NBD2 R621Q in complex with AMPPCP, PDB entry 4lj9 , contour level 1.5σ. (f) ClpB NBD2 R621Q in complex with ADP, PDB entry 4lj8 , contour level 1.5σ. (g) ClpB NBD2 R621Q in complex with AMPPCP and GdmCl, PDB entry 4lja , contour level 1.5σ. For detailed structure analyses, compare Figs. 2[link], 3[link], 5[link] and 6[link].

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 70| Part 2| January 2014| Pages 582-595
doi:10.1107/S1399004713030629
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