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Figure 6
Residual activities of BGLPf-M4a (M4a), BGLPf-M4b (M4b), BGLPf-M4c (M4c), BGLPf-WT (WT) and BGLPf-M3 (M3) after heating. Purified enzymes (1.0 mg ml−1) were incubated for 10 min in 50 mM Tris–HCl buffer pH 7.2 at the given temperatures. The residual activity of each enzyme after heating was assayed under standard conditions with 10 mM cellobiose for 10 min at 40°C. Activity was expressed as the concentration of glucose produced (%).

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 70| Part 3| February 2014| Pages 877-888
doi:10.1107/S1399004713032276
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