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Figure 1
The SmVAL4 monomer. (a) A Coomassie-stained SDS gel reveals the purity of the SmVAL4 sample and its monomeric mass of ∼20 kDa. Lane M contains molecular-weight marker (labeled in kDa). (b) SEC-MALS analysis reveals that SmVAL4 is an ∼20 kDa monomer in solution. (c) Fit of N-­glycosylated Asn118 and proximal residues into a 2FoFc electron-density map (gray) calculated from the refined model of SmVAL4 and contoured at 1.2σ. NAG, N-acetyl-D-glucosamine. (d) Topology of the SmVAL4 structure.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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