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Figure 2
ITC raw heat pulse data for the association of UNG with Ugi at 25°C in 20 mM sodium phosphate, 200 mM NaCl pH 7.5. The heat was measured upon injecting 20 5 µl aliquots of Ugi into a 997 µl reaction cell containing UNG. 667 µM Ugi was titrated into the buffer in (a) and 50 µM hUNG in (b); 267 µM Ugi was titrated into the buffer in (c) and 20 µM cUNG in (d).

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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