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Figure 4
NO66 recognizes Rpl8 through a consensus motif. (a) A sequence alignment of the fragment in Rpl8 which is recognized by NO66 is shown. The accession numbers of the protein sequences are Homo sapiens, NP_150644 (human); Macaca mulatta, NP_001253030 (rhesus macaque); Bos taurus, NP_001029797 (cattle); Mus musculus, NP_036183 (mouse); Rattus norvegicus, NP_001030088 (rat); Danio rerio, NP_957007 (fish); Drosophila simulans, NP_728756 (fly). The residues that are critical for the binding of Rpl8 to NO66 are highlighted in red. (b) Pull-down of NO66176–C by wild-type Rpl8193–C and mutants of Rpl8193–C fused to GST are shown.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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