High-density grids for efficient data collection from multiple crystals

Baxter, E.L.; Aguila, L.; Alonso-Mori, R.; Barnes, C.O.; Bonagura, C.A.; Brehmer, W.; Brunger, A.T.; Calero, G.; Caradoc-Davies, T.T.; Chatterjee, R.; Degrado, W.F.; Fraser, J.S.; Ibrahim, M.; Kern, J.; Kobilka, B.K.; Kruse, A.C.; Larsson, K.M.; Lemke, H.T.; Lyubimov, A.Y.; Manglik, A.; McPhillips, S.E.; Norgren, E.; Pang, S.S.; Soltis, S.M.; Song, J.; Thomaston, J.; Tsai, Y.; Weis, W.I.; Woldeyes, R.A.; Yachandra, V.; Yano, J.; Zouni, A.; Cohen, A.E.

doi:10.1107/S2059798315020847

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 3
(a) A grid incubating inside a vapor-diffusion chamber. Lysozyme crystals are growing in sitting drops on the grid. (b) An expanded view of the grid vapor-diffusion chamber. The grid (white) containing sample is held within the crystallization chamber, while the magnetic base (silver) sits in an external cutout. An X-seal crystallization support screws into the crystallization plate to close the chamber. Desiccant may be held below the grid in the chamber. Silicone O-rings (red) create a tight seal around the grid. (c) Sitting drops on a grid incubating inside a vapor-diffusion chamber. A thin sheet of polycarbonate was affixed to one face of the grid and a solution of lysozyme in precipitant was dispensed onto the grid backing inline with grid ports. Lysozyme crystals can be seen growing in the sitting drops.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 72| Part 1| January 2016| Pages 2-11

https://doi.org/10.1107/S2059798315020847

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