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![[Figure 5]](ba5246fig5mag.jpg)
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Figure 5
Representative electron density for CSN. A portion of the electron-density map (grey mesh) surrounding the active site of CSN5 (cyan) calculated at 4 Å resolution and contoured at 1 r.m.s. for crystal c337 by cross-crystal averaging with NCS averaging across the three non-isomorphous refinement data sets with RESOLVE. The structure-factor amplitudes were sharpened by −134 Å2 (isotropic B factor 40 Å2) before density modification. Cα traces are shown for CSN5 (cyan) superposed with the high-resolution structure of CSN5 (red; PDB entry 4f7o ) and CSN6 (orange). CSN5 assumes an auto-inhibited conformation in CSN, which was not found for the crystal of the CSN5 fragment on its own. This results in conformational differences at the active site (indicated by a red sphere for the active-site Zn2+ ion) of the two CSN5 models. |
![[Figure 5]](ba5246fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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