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Figure 3
CrystalDirect robot control software. The signal from a video camera monitoring the laser working area within a microplate (centre) is used to locate the aperture (green trace) and the cutting shape (red line) and to select the final position of the tip of the pin relative to the crystal (red oval). Another camera monitors the position of the cryocooling jet (top left), allowing inspection of the samples at the end of the harvesting and cryocooling process. Images and coordinates of crystals can be stored and automatically recovered by the harvester software (bottom left). Alternatively, operators can select any position within a 96-well microplate (bottom right). The software provides full control over the harvesting and processing parameters and records the final location of the harvested samples within standard SPINE sample pucks (bottom, centre). The system also monitors a number of processes such as, for example, calibration of the pin position and delivery of a glue droplet (right middle).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 72| Part 4| April 2016| Pages 454-466
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